Human CRP(C-Reactive Protein) ELISA Kit

Alternative product: E-TSEL-H0007
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-H0043

      Synonyms:PTX1, Pentraxin 1

      • 96T
      • 24T
      - +
      Price: $495

      Reactivity: Human

      Detection Range: 0.39~25 ng/mL

      Sensitivity: 0.23 ng/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

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      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Human CRP concentrations in Serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CRP. You can calculate the concentration of Human CRP in the samples by comparing the OD of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Human
      Detection Method Colormetric
      Detection Range 0.39—25 ng/mL
      Sensitivity 0.23 ng/mL
      Sample Volume Required Per Well 100μL
      Sample Type Serum, plasma and other biological fluids


      This kit recognizes Human CRP in samples. No significant cross-reactivity or interference between Human CRP and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      O.D Average Corrected
      25 2.33
      2.346 2.284
      12.5 1.522
      1.552 1.49
      6.25 0.933
      0.921 0.859
      3.12 0.438
      0.438 0.376
      1.56 0.266
      0.259 0.197
      0.78 0.164
      0.159 0.097
      0.39 0.11
      0.112 0.05
      0 0.055
      0.062 --


      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CRP were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CRP were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (ng/mL) 2.50 4.90 16.80 2.50 4.70 18.20
      Standard deviation 0.10 0.20 0.60 0.20 0.30 0.70
      C V (%) 4.00 4.08 3.57 8.00 6.38 3.85


      The recovery of Human CRP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 90-102 95
      EDTA plasma (n=5) 85-97 90
      Cell culture media (n=5) 88-103 94


      Samples were spiked with high concentrations of Human CRP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 95-111 92-106 98-109
      Average (%) 103 98 103
      1:4 Range (%) 97-112 85-96 90-105
      Average (%) 105 91 97
      1:8 Range (%) 98-111 86-100 93-111
      Average (%) 105 93 101
      1:16 Range (%) 99-113 87-101 98-114
      Average (%) 105 92 104

      Kit components & Storage

      An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20°C, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20°C(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 2-8°C(shading light)
      Stop Solution 1 vial, 10 mL 2-8°C
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37°C
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

      elisa assay procedure 2

      2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

      elisa assay procedure 3

      3. Aspirate and wash the plate for 3 times

      elisa assay procedure 4

      4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

      elisa assay procedure 5

      5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

      elisa assay procedure 6

      6. Add 50μL Stop Solution

      elisa assay procedure 7

      7. Read the plate at 450nm immediately. Calculation of the results


      1. High Anti-Human Cytomegalovirus Antibody Levels Are Associated With The Progression Of Essential Hypertension And Target Organ Damage In Han Chinese Population.
        IF: 2.806
        Journal: Plos One(2017)
        DOI: 10.1371/journal.pone.0181440
        PMID: 28837559
        Products: E-EL-H0043 E-EL-H0109 E-EL-H0102 
        Sample type: Serum
        Reactivity: Human
      2. TSG Attenuates LPC-Induced Endothelial Cells Inflammatory Damage Through Notch Signaling Inhibition.
        IF: 3.141
        Journal: IUBMB Life(2016)
        DOI: 10.1002/iub.1458
        PMID: 26662286
        Products: E-EL-H0102 E-EL-H0043 
        Sample type: Cell culture supernatant
        Reactivity: Human
      3. Prognostic Significance of Serum NGAL and Troponin I against Acute Kidney Injury in Egyptian ICU Patients after Open Heart Surgery: A Pilot Study.
        IF: /
        Journal: Kidney Diseases(2018)
        DOI: 10.1159/000490246
        PMID: 30574501
        Products: E-EL-H0043 
        Sample type: Serum
        Reactivity: Human
      4. Biochemical investigation of gender-specific association between insulin resistance and inflammatory biomarkers in types 2 diabetic patients.
        IF: 3.457
        Journal: Biomedicine & Pharmacotherapy(2018)
        DOI: 10.1016/j.biopha.2018.06.044
        PMID: 29966972
        Products: E-EL-H0102 E-EL-H0109 E-EL-H0043 E-EL-H5439 
        Sample type: Serum
        Reactivity: Human
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