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Human Cys-C(Cystatin C) ELISA Kit

  • Cat.No.:E-EL-H3643

  • Reactivity: Human

To Purchase E-EL-H3643

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human Cys-C in samples. No significant cross-reactivity or interference between Human Cys-C and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human Cys-C concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Cys-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Cys-C. You can calculate the concentration of Human Cys-C in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human Cys-C were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human Cys-C were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 2.30 6.80 15.60 2.30 7.50 15.60
Standard deviation 0.09 0.12 0.23 0.10 0.16 0.24
CV (%) 4.55 4.22 5.28 6.13 5.73 4.29

Recovery

The recovery of Human Cys-C spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-105 98
EDTA plasma (n=8) 94-110 100
Cell culture media (n=8) 92-101 97

Linearity

Samples were spiked with high concentrations of Human Cys-C and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsCST3, Cystatin 3, ARMD11, Post-Gamma-Globulin
Research AreaCancer, Cell Biology, Cardiovascular, Stem cells, Tags and Cell Markers

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. SAUDI MEDICAL JOURNAL (2020) IF: 1.195
    Evaluation of early biomarkers of renal dysfunction in diabetic patients.

    DOI: 10.15537/smj.2020.7.25168

    Sample: Serum
  2. Genetic Testing and Molecular Biomarkers (2020) IF: 1.378
    Correlation Between Single Nucleotide Polymorphisms at the 3′-UTR of the NFKB1 Gene and Acute Kidney Injury in Sepsis

    DOI: 10.1089/gtmb.2019.0222

    Sample: Cell Sample
  3. MEDICINE (2018) IF: 2.028
    Long-term benefits of high-intensity atorvastatin therapy in Chinese acute coronary syndrome patients undergoing percutaneous coronary intervention: A retrospective study

    DOI: 10.1097/MD.0000000000012687

    PMID: 30334951

    Sample: Serum
  4. PLoS One (2021) IF: 3.24
    Effects of pneumoperitoneum on kidney injury biomarkers: A randomized clinical trial

    DOI: 10.1371/journal.pone.0247088

    PMID: 33606739

    Sample: Plasma
  5. Biomed Research International (2022) IF: 3.246
    Evaluation Value of Serum miR-4299 and miR-16-5p in Risk Stratification of Sepsis-Induced Acute Kidney Injury

    DOI: 10.1155/2022/5165892

    PMID: 35845963

    Sample: Serum
  6. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2016) IF: 3.257
    Label-Free Quantitative Proteomics Reveals Differences in Molecular Mechanism of Atherosclerosis Related and Non-Related to Chronic Kidney Disease

    DOI: 10.3390/ijms17050631

    Sample: Plasma
  7. Nutrients (2018) IF: 4.196
    Tocotrienol-Rich Vitamin E from Palm Oil (Tocovid) and Its Effects in Diabetes and Diabetic Nephropathy: A Pilot Phase II Clinical Trial

    DOI: 10.3390/nu10091315

    PMID: 30227659

    Sample: Serum
  8. JOURNAL OF NEPHROLOGY (2023) IF: 4.393
    Associations of prematurity and low birth weight with blood pressure and kidney function in middle-aged participants of the Brazilian Longitudinal Study of Adult Health: ELSA-Brasil

    DOI: 10.1007/s40620-022-01549-w

    Sample: Serum
  9. Journal of Inflammation Research (2021) IF: 6.922
    Endothelin-1/Endothelin Receptor Type A-Angiopoietins/Tie-2 Pathway in Regulating the Cross Talk Between Glomerular Endothelial Cells and Podocytes in Trichloroethylene-Induced Renal Immune Injury

    DOI: 10.2147/JIR.S301104

    Sample: Serum,Plasma,Urine
  10. Translational Research (2022) IF: 7.012
    Interplay between mineral bone disorder and cardiac damage in acute kidney injury: from Ca2+ mishandling and preventive role of Klotho in mice to its potential mortality prediction in human

    DOI: 10.1016/j.trsl.2022.01.002

    PMID: 35077866

    Sample: Plasma
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