Human DAO (Diamine Oxidase) ELISA Kit (E-EL-H6285)

Name: Human DAO (Diamine Oxidase) ELISA Kit
Brand: Elabscience
SKU: E-EL-H6285
Price: 150.0 USD
Availability: InStock

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Human DAO (Diamine Oxidase) ELISA Kit - 1

Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes DAO in samples.No significant cross-reactivity or interference between DAO and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	2.332	2.268	2.300	2.221
1000	1.543	1.583	1.563	1.484
500	0.902	0.878	0.890	0.811
250	0.538	0.546	0.542	0.463
125	0.305	0.287	0.296	0.217
62.5	0.186	0.176	0.181	0.102
31.25	0.130	0.142	0.136	0.057
0	0.081	0.077	0.079	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	101.060	208.830	909.000	91.890	193.870	840.750
Standard deviation	6.280	9.000	45.540	6.180	13.730	73.900
CV（%）	6.210	4.310	5.010	6.720	7.080	8.790

Recovery

The recovery of Human DAO was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Cell culture media （n=8）	88-103	94
Serum （n=8）	89-102	95
EDTA plasma （n=8）	83-95	90

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human DAO and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	84-97	97-113	98-115
1:2	Average	91	105	106
1:4	Range	85-97	96-111	99-116
1:4	Average	91	104	107
1:8	Range	83-97	96-112	101-116
1:8	Average	89	104	108

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	96.74-103.39
Sample 2（n=16）	85.16-112.46

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to DAO . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DAO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DAO , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of DAO . You can calculate the concentration of DAO in the samples by comparing the OD of the samples to the standard curve.

Background

This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia.