Human DS(Dermatan Sulfate) ELISA Kit (E-EL-H1725)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DS. You can calculate the concentration of Human DS in the samples by comparing the OD of the samples to the standard curve.

Dermal sulfate (DS) is a disaccharide polymer, one of the most widely distributed extra-cell matrix (ecm) glycosaminoglycans in animals, and the main component of blood vessel wall proteoglycans.DS, as an important component of connective tissue, has a variety of pharmacological and physiological functions, and can be used as drugs and health food, mainly for osteoarthritis (oa) and coronary atherosclerotic heart disease (coronary heart disease).