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Human ECF/CCL11(Eosinophil Chemotactic Factor) ELISA Kit

Citations(10)Uniprot : P51671

Cat.No.:E-EL-H0025
Reactivity: Human

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96T
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96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human ECF/CCL11 in samples. No significant cross-reactivity or interference between Human ECF/CCL11 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human ECF/CCL11 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ECF/CCL11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ECF/CCL11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ECF/CCL11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ECF/CCL11. You can calculate the concentration of Human ECF/CCL11 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ECF/CCL11 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ECF/CCL11 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	51.30	124.70	336.30	46.80	127.70	341.40
Standard deviation	2.60	5.10	10.10	2.90	6.40	16.40
CV (%)	5.07	4.09	3.00	6.20	5.01	4.80

Recovery

The recovery of Human ECF/CCL11 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	96-108	101
EDTA plasma (n=8)	85-97	91
Cell culture media (n=8)	91-106	98

Linearity

Samples were spiked with high concentrations of Human ECF/CCL11 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P51671
Synonyms	Eotaxin 1, SCYA11
Research Area	Cancer, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

SCHIZOPHRENIA BULLETIN (2021) IF: 9.3059
High Mobility Group Protein 1 and Dickkopf-Related Protein 1 in Schizophrenia and Treatment-Resistant Schizophrenia: Associations With Interleukin-6, Symptom Domains, and Neurocognitive Impairments

DOI: 10.1093/schbul/sbaa136

Sample: serum
JOURNAL OF AFFECTIVE DISORDERS (2021) IF: 4.839
In schizophrenia, immune-inflammatory pathways are strongly associated with depressive and anxiety symptoms, which are part of a latent trait which comprises neurocognitive impairments and schizophrenia symptoms.

DOI: 10.1016/j.jad.2021.03.062

Sample: Serum
Bioengineered (2021) IF: 3.269
Apremilast mitigates interleukin (IL)-13-induced inflammatory response and mucin production in human nasal epithelial cells (hNECs)

DOI: 10.1080/21655979.2021.1987818

PMID: 34607526
Sample: Human nasal epithelial cells (hNECs)
METABOLIC BRAIN DISEASE (2021) IF: 3.584
In schizophrenia, non-remitters and partial remitters to treatment with antipsychotics are qualitatively distinct classes with respect to neurocognitive deficits and neuro-immune biomarkers: results of soft independent modeling of class analogy

DOI: 10.1007/s11011-021-00685-9

PMID: 33580860
Sample: Plasma
SCHIZOPHRENIA RESEARCH (2020) IF: 3.759
Chronic fatigue and fibromyalgia symptoms are key components of deficit schizophrenia and are strongly associated with activated immune-inflammatory pathways

DOI: 10.1016/j.schres.2020.05.003

PMID: 32467068
Sample: Serum
JOURNAL OF AFFECTIVE DISORDERS (2020) IF: 3.892
Construction of an exposure-pathway-phenotype in children with depression due to transfusion-dependent thalassemia: Results of (un)supervised machine learning.

DOI: 10.1016/j.jad.2020.12.089

Sample: Serum
METABOLIC BRAIN DISEASE (2020) IF: 2.726
Chronic fatigue syndrome and fibromyalgia-like symptoms are an integral component of the phenome of schizophrenia: neuro-immune and opioid system correlates

DOI: 10.1007/s11011-020-00619-x

PMID: 32965599
Sample: Serum
NEUROTOXICITY RESEARCH (2020) IF: 2.992
The Neuroimmune and Neurotoxic Fingerprint of Major Neurocognitive Psychosis or Deficit Schizophrenia: a Supervised Machine Learning Study

DOI: 10.1007/s12640-019-00112-z

Sample: serum
NEUROTOXICITY RESEARCH (2020) IF: 2.992
Major Depression in Children with Transfusion-Dependent Thalassemia Is Strongly Associated with the Combined Effects of Blood Transfusion Rate, Iron Overload, and Increased Pro-inflammatory Cytokines

DOI: 10.1007/s12640-020-00193-1

PMID: 32335809
Sample: Serum
Brazilian Journal of Psychiatry (2020) IF: 1.904
Lowered serum cesium levels in schizophrenia: association with immune-inflammatory biomarkers and cognitive impairments

DOI: 10.1590/1516-4446-2020-0908

PMID: 32556004
Sample: Serum

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