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Human ESM1(Endothelial Cell Specific Molecule 1) ELISA Kit

  • Cat.No.:E-EL-H1557

  • Reactivity: Human

To Purchase E-EL-H1557

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human ESM1 in samples. No significant cross-reactivity or interference between Human ESM1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human ESM1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ESM1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ESM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ESM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ESM1. You can calculate the concentration of Human ESM1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ESM1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ESM1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 54.84 154.68 423.76 52.25 168.48 438.78
Standard deviation 3.49 8.80 13.01 3.18 10.08 22.20
CV (%) 6.36 5.69 3.07 6.09 5.98 5.06


The recovery of Human ESM1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-108 100
EDTA plasma (n=8) 86-101 92
Cell culture media (n=8) 92-105 98


Samples were spiked with high concentrations of Human ESM1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q9NQ30
Synonymsendocan,Endothelial cell-specific molecule 1
Research AreaTags and Cell Markers

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. European Journal of Rheumatology (2018) IF: 3.15
    The Relationship of Serum Endocan Levels and Anti-TNF-alpha Therapy in Patients with Ankylosing Spondylitis.

    DOI: 10.5152/eurjrheum.2017.17287

    PMID: 29657867

  2. The Journal of Maternal-Fetal & Neonatal Medicine (2018) IF: 1.569
    Serum endocan concentration and its correlation with severity of hypertensive disorders in pregnancy

    DOI: 10.1080/14767058.2018.1548597

    PMID: 30501553

    Sample: Serum
  3. Journal of perinatal medicine (2019) IF: 1.558
    Maternal Serum endocan concentrations are elevated in patients with preterm premature rupture of membranes

    DOI: 10.1515/jpm-2018-0401

    PMID: 30875331

    Sample: Serum
  4. Journal of Obstetrics and Gynaecology (2020) IF: 0.8070
    Endothelial cell-specific-molecule-1 (endocan) levels in women with premature ovarian insufficiency: a prospective comparative study

    DOI: 10.1080/01443615.2020.1789952

    PMID: 32808833

    Sample: Serum
  5. Pakistan journal of medical sciences (2019) IF: 0.719
    The role of Endocan as a Prognostic Biomarker in community-acquired pneumonia

    DOI: 10.12669/pjms.35.1.280

    PMID: 30881408

    Sample: Serum
  6. The journal of international advanced otology (2018)
    Evaluation of Serum Endocan Levels in Sensorineural Hearing Loss

    DOI: 10.5152/iao.2018.5051

    PMID: 30325334

    Sample: Serum
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