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Human ET-1(Endothelin 1) ELISA Kit

  • Cat.No.:E-EL-H0064

  • Reactivity: Human

To Purchase E-EL-H0064

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 1.25-80 pg/mL
Sensitivity 0.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human ET-1 in samples. No significant cross-reactivity or interference between Human ET-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human ET-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ET-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ET-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ET-1. You can calculate the concentration of Human ET-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ET-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ET-1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 4.00 7.80 33.60 4.00 7.70 36.30
Standard deviation 0.30 0.40 1.40 0.20 0.30 1.40
CV (%) 7.50 5.13 4.17 5.00 3.90 3.86

Recovery

The recovery of Human ET-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-103 97
EDTA plasma (n=8) 89-104 96
Cell culture media (n=8) 86-100 92

Linearity

Samples were spiked with high concentrations of Human ET-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P05305
SynonymsEDN1, ET1, HDLCQ7, PPET1, Preproendothelin-1
Research AreaCancer, Cardiovascular, Metabolism, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Frontiers in Pharmacology (2021) IF: 5.811
    Sinapine Thiocyanate Ameliorates Vascular Endothelial Dysfunction in Hypertension by Inhibiting Activation of the NLRP3 Inflammasome

    DOI: 10.3389/fphar.2020.620159

    PMID: 33633569

    Sample: Cell Culture Supernatant
  2. Frontiers in Pharmacology (2021) IF: 5.811
    Identification of the Antithrombotic Mechanism of Leonurine in Adrenalin Hydrochloride-Induced Thrombosis in Zebrafish via Regulating Oxidative Stress and Coagulation Cascade.

    DOI: 10.3389/fphar.2021.742954

  3. Brazilian Journal of Cardiovascular Surgery (2020) IF: 1.053
    Comparison between the Effects of Bretschneider’s HTK Solution and Cold Blood Cardioplegia on Systemic Endothelial Functions in Patients who Undergo Coronary Artery Bypass Surgery: a Prospective Randomized and Controlled Trial

    DOI: 10.21470/1678-9741-2019-0327

    Sample: Serum
  4. Journal of Maternal-Fetal & Neonatal Medicine (2017) IF: 1.826
    Serum endothelin-1 and placental alkaline phosphatase levels in placenta percreta and normal pregnancies

    DOI: 10.1080/14767058.2017.1297412

    Sample: Serum
  5. MEDICAL SCIENCE MONITOR (2019) IF: 1.98
    Protective Effects of Dexmedetomidine and Oxycodone in Patients Undergoing Limb Ischemia-Reperfusion

    DOI: 10.12659/MSM.918261

    PMID: 31782408

    Sample: Serum,Plasma
  6. PHARMACOGENOMICS (2019) IF: 2.265
    Association of endothelin receptor type A rs5333 gene polymorphism with steroid response in Egyptian children with idiopathic nephrotic syndrome

    DOI: 10.2217/pgs-2018-0175

    PMID: 30672385

    Sample: Plasma
  7. International Journal of Medical Sciences (2020) IF: 2.523
    Shorter Leukocyte Telomere Length coupled with lower expression of Telomerase Genes in patients with Essential Hypertension

    DOI: 10.7150/ijms.48456

    Sample: Plasma
  8. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
    Isoflavones from Semen Sojae Preparatum Improve Atherosclerosis and Oxidative Stress by Modulating Nrf2 Signaling Pathway through Estrogen-Like Effects

    DOI: 10.1155/2022/4242099

    PMID: 35432565

    Sample: Serum,Ea Hy926 Cell
  9. Polish Archives of Internal Medicine-Polskie Archiwum Medycyny Wewnetrznej (2018) IF: 2.658
    High‑density lipoprotein cholesterol levels and pulmonary artery vasoreactivity in patients with idiopathic pulmonary arterial hypertension

    DOI: 10.20452/pamw.4306

    Sample: Plasma
  10. JOURNAL OF NEPHROLOGY (2018) IF: 2.724
    The association of endothelin-1 levels with renal survival in polycystic kidney disease patients

    DOI: 10.1007/s40620-018-0514-2

    Sample: Serum
  11. Medicina-Lithuania (2022) IF: 2.948
    Changes in Left Heart Geometry, Function, and Blood Serum Biomarkers in Patients with Obstructive Sleep Apnea after Treatment with Continuous Positive Airway Pressure

    DOI: 10.3390/medicina58111511

    PMID: 36363468

    Sample: Serum
  12. American Journal of Translational Research (2021) IF: 4.06
    Minimally-invasive stone removal in urinary calculi patients during pregnancy: clinical therapeutic effects and low complications

    PMID: 34540086

    Sample: Serum
  13. Nanoscale (2019) IF: 6.97
    Atmospheric nanoparticles affect vascular function using a 3D human vascularized organotypic chip

    DOI: 10.1039/C9NR03622A

    Sample: Microvessels Cells
  14. Antioxidants (2022) IF: 7.675
    Natural Activators of Autophagy Increase Maximal Walking Distance and Reduce Oxidative Stress in Patients with Peripheral Artery Disease: A Pilot Study

    DOI: 10.3390/antiox11091836

    PMID: 36139910

    Sample: Serum
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