Human Fcγ(Fc Fragment of IgG) ELISA Kit (E-EL-H1745)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Fcγ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Fcγ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Fcγ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Fcγ. You can calculate the concentration of Human Fcγ in the samples by comparing the OD of the samples to the standard curve.

Immunoglobulin (Ig) is a protein secreted by effector B cells under antigen stimulation of the immune system that can bind specifically to the corresponding antigen. Immunoglobulins are composed of two distinct functional units Fab and Fc fragments. The Fab fragment contains three variable complementary determinant regions for binding specific epitopes; Fc fragments are involved in the regulation of the immune system in a variety of ways.