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Human FSH(Follicle Stimulating Hormone) ELISA Kit

Citations(7)Uniprot : P01225

Cat.No.:E-EL-H1143
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	1.56-100 mIU/mL
Sensitivity	0.94 mIU/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human FSH in samples. No significant cross-reactivity or interference between Human FSH and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human FSH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FSH. You can calculate the concentration of Human FSH in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human FSH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human FSH were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (mIU/mL)	4.90	12.20	42.60	4.90	11.10	46.20
Standard deviation	0.30	0.60	1.60	0.30	0.60	1.50
CV (%)	6.12	4.92	3.76	6.12	5.41	3.25

Recovery

The recovery of Human FSH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	85-96	91
EDTA plasma (n=8)	90-101	95
Cell culture media (n=8)	92-103	97

Linearity

Samples were spiked with high concentrations of Human FSH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P01225
Research Area	Cancer, Metabolism, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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Acta Neuropathologica Communications (2023) IF: 7.578

Identification and gene expression profiling of human gonadotrophic pituitary adenoma stem cells

DOI: 10.1186/s40478-023-01517-w
	Sample: human gonadotrophic pituitary adenoma stem cells (

Cells (2022) IF: 6.6

Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway

DOI: 10.3390/cells11101690
PMID: 35626727	Sample: serum

Cell Death & Disease (2017) IF: 5.965

ANP promotes proliferation and inhibits apoptosis of ovarian granulosa cells by NPRA/PGRMC1/EGFR complex and improves ovary functions of PCOS rats

DOI: 10.1038/cddis.2017.494
	Sample: Serum

JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36

Evaluation of possible effects of Persea americana seeds on female reproductive hormonal and toxicity profile

DOI: 10.1016/j.jep.2021.113870
PMID: 33484907	Sample: Serum

TOXICOLOGY AND APPLIED PHARMACOLOGY (2019) IF: 3.585

High copper levels in follicular fluid affect follicle development in polycystic ovary syndrome patients: Population-based and in vitro studies

DOI: 10.1016/j.taap.2019.01.008
PMID: 30641075	Sample: Serum,Cell culture medium

REPRODUCTION (2021) IF: 3.906

Melatonin ameliorates ovarian dysfunction by regulating autophagy in PCOS via the PI3K-Akt pathway

DOI: 10.1530/REP-20-0643
PMID: 33989172	Sample: serum

ANDROLOGIA (2022) IF: 2.532

The impact of dolutegravir-based combination antiretroviral therapy on the spermatozoa and fertility parameters of men living with human immunodeficiency virus

DOI: 10.1111/and.14621
PMID: 36261884	Sample: serum

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Reviews/Q&A

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Q&A

A***********SSubmitted [ Mar 11 2023 ]

Asked: Can this kit be used to test salivary FSH? And what considerations should be taken (should saliva be centrifuged first? Saliva storage before using it on this kit?)

adminSubmitted [ Mar 11 2023 ]

Answered: Hi Abdulrahman, We have verified human saliva samples with E-EL-H1143, and the detection value are very low, and most of the OD values are no signal. It is not recommended to use this kit to detect saliva samples. Here are the recommendations for collection and preservation of saliva samples: Patients should fast and gargle one hour before sampling to keep the mouth clean. Saliva samples should be centrifuged at 2-8℃, 12000r/min for 10 minutes to remove impurities, and take the supernatant for detection. It is recommended to use fresh saliva samples for testing. Samples could be stored for one month at - 20 ℃ and three months at - 80 ℃. If any further questions, pls email to customer00@elabscience.com Best regards

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