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Human GAL3(Galectin 3) ELISA Kit

  • Cat.No.:E-EL-H1470

  • Reactivity: Human

To Purchase E-EL-H1470

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human GAL3 in samples. No significant cross-reactivity or interference between Human GAL3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human GAL3 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GAL3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GAL3. You can calculate the concentration of Human GAL3 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human GAL3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human GAL3 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.55 1.03 3.99 0.54 0.96 3.64
Standard deviation 0.04 0.05 0.18 0.03 0.05 0.20
CV (%) 7.27 4.85 4.51 5.56 5.21 5.49

Recovery

The recovery of Human GAL3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-104 99
EDTA plasma (n=8) 89-104 96
Cell culture media (n=8) 94-107 100

Linearity

Samples were spiked with high concentrations of Human GAL3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P17931
SynonymsLGALS3, CBP35, GALBP, GALIG, L31, LGALS2, MAC2, Lectin,Galactoside-Binding Soluble 3
Research AreaCell biology,Neuroscience,Epigenetics and Nuclear Signaling

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of Cardiovascular Magnetic Resonance (2020) IF: 5.361
    Imaging, biomarker and invasive assessment of diffuse left ventricular myocardial fibrosis in atrial fibrillation

    DOI: 10.1186/s12968-020-0603-y

    PMID: 32036784

    Sample: Whole Blood
  2. Frontiers in Physiology (2020) IF: 3.201
    Elevated b1-Adrenergic Receptor Autoantibody Levels Increase Atrial Fibrillation Susceptibility by Promoting Atrial Fibrosis

    DOI: 10.3389/fphys.2020.00076

    PMID: 32116783

    Sample: Serum
  3. Clinical Biochemistry (2016) IF: 2.434
    Circulating Biomarkers Of Fibrosis And Cardioversion Of Atrial Fibrillation: A Prospective, Controlled Cohort Study.

    DOI: 10.1016/j.clinbiochem.2016.09.008

    PMID: 27622867

    Sample: Serum
  4. Brazilian Journal of Psychiatry (2020) IF: 1.904
    Serum galectin-3 levels are decreased in schizophrenia

    DOI: 10.1590/1516-4446-2019-0699

    PMID: 32159713

    Sample: Serum
  5. Psychiatry Investigation (2020) IF: 1.688
    Serum Galectin-3 Levels in Children with Attention-Deficit/Hyperactivity Disorder

    DOI: 10.30773/pi.2019.0247

    PMID: 32151128

    Sample: Serum
  6. Medical ultrasonography (2018) IF: 1.533
    The value of global longitudinal strain and galectin-3 for predicting cardiovascular events in patients with severe aortic stenosis

    DOI: 10.11152/mu-1456

    PMID: 29730688

    Sample: Serum,Plasma
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