Human GC(Glucagon) ELISA Kit (E-EL-H2237)
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For research use only.
Product Summary
| Sensitivity | 18.75 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Sample Volume | 50 μL |
| Total Assay Time | 2 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes Human GC in samples.No significant cross-reactivity or interference between Human GC and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Competitive |
| Assay Type | Competitive-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Test Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human GC. During the reaction, Human GC in the sample or standard competes with a fixed amount of Human GC on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human GC. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human GC in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Background
| Gene ID | 2641 |
| Uniport ID | P01275 |
| Research Area | Cancer , Metabolism , Developmental Biology , Signal Transduction , Stem Cells |
| Cat.No. | Product Name | Clone No. |
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