Human HMGB-1(High Mobility Group Protein B1) ELISA Kit

    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-H1554

      Synonyms:HMGB1, HMG1, HMG3, SBP-1, Amphoterin

      • 96T
      • 24T
      - +
      Price: $580

      Reactivity: Human

      Detection Range: 31.25~2000 pg/mL

      Sensitivity: 18.75 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Human HMGB-1 concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HMGB-1. You can calculate the concentration of Human HMGB-1 in the samples by comparing the OD of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Human
      Detection Method Colormetric
      Detection Range 31.25—2000 pg/mL
      Sensitivity 18.75 pg/mL
      Sample Volume Required Per Well 100μL
      Sample Type Serum, plasma and other biological fluids


      This kit recognizes Human HMGB-1 in samples. No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      O.D Average Corrected
      2000 2.443
      2.445 2.374
      1000 1.718
      1.741 1.67
      500 0.988
      0.986 0.915
      250 0.466
      0.482 0.411
      125 0.265
      0.256 0.185
      62.5 0.187
      0.174 0.103
      31.25 0.118
      0.124 0.053
      0 0.069
      0.071 --


      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human HMGB-1 were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human HMGB-1 were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (pg/mL) 99.32 202.75 693.26 108.96 208.03 746.59
      Standard deviation 5.84 8.62 35.56 5.86 9.76 26.06
      C V (%) 5.88 4.25 5.13 5.38 4.69 3.49


      The recovery of Human HMGB-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 94-108 100
      EDTA plasma (n=5) 94-107 100
      Cell culture media (n=5) 93-105 99


      Samples were spiked with high concentrations of Human HMGB-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 98-111 92-105 85-100
      Average (%) 105 98 92
      1:4 Range (%) 90-103 84-100 88-102
      Average (%) 98 91 94
      1:8 Range (%) 87-99 83-93 85-96
      Average (%) 94 88 90
      1:16 Range (%) 88-103 85-96 86-99
      Average (%) 94 90 91

      Kit components & Storage

      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 4℃(shading light)
      Stop Solution 1 vial, 10 mL 4℃
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37℃
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1.Add 100 μL standard or sample to each well. Incubate for 90 min at 37℃.

      elisa assay procedure 2

      2.Remove the liquid.

      elisa assay procedure 3

      3.Add 100 μL Biotinylated Detection Ab. Incubate for 1 hour at 37℃. Aspirate and wash 3 times.

      elisa assay procedure 4

      4.Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

      elisa assay procedure 5

      5.Add 90 μL of Substrate Reagent. Incubate for 15 min at 37℃.

      elisa assay procedure 6

      6.Add 50 μL Stop Solution.

      elisa assay procedure 7

      7.Read at 450 nm immediately. Calculation of results.


      1. Publication: Wang X, Chu G, Yang Z, et al. Ethanol Directly Induced HMGB1 Release Through NOX2/NLRP1 Inflammasome In Neuronal Cells[J]. Toxicology, 2015, 334: 104-110.
        Sample Type: culture supernatant
      2. Publication: Zheng H, Chen J N, Yu X, et al. Hmgb1 Enhances Drug Resistance And Promotes In Vivo Tumor Growth Of Lung Cancer Cells[J]. DNA and Cell Biology, 2016, 35(10): 622-627.
        Sample Type: Cell supernatant and Serum
      3. Publication: Guan X, Wang P, Chi J, et al. Relationships Of BRAF Mutation And HMGB1To Papillary Thyroid Carcinoma[J]. Biochemical And Biophysical Research Communications, 2017, 486(4): 898-903.
        Sample Type: Serum
      4. Publication: Pfalzgraff A, Heinbockel L, Su Q, et al. Synthetic Anti-Endotoxin Peptides Inhibit Cytoplasmic LPS-Mediated Responses[J]. Biochemical Pharmacology, 2017.
        Sample Type: culture supernatant
      5. Publication: Yang J Y, Ma Z Q, Wang Y L, et al. Necrosis Of Osteosarcoma Cells Induces The Production And Release Of High‑Mobility Group Box 1 Protein[J]. Experimental and Therapeutic Medicine, 2018, 15: 461-466.
        Sample Type: culture supernatant
      6. Publication: Orio L, Antón M, Rodríguez-Rojo I C, et al. Young Alcohol Binge Drinkers Have Elevated Blood Endotoxin, Peripheral Inflammation And Low Cortisol Levels: Neuropsychological Correlations In Women[J]. Addiction Biology, 2017.
        Sample Type: Plasma
      7. Publication: Yu H, Dong R, Lu Y Y, et al. Short-Term Postoperative Cognitive Dysfunction And Inflammatory Response In Patients Undergoing Cytoreductive Surgery And Hyperthermic Intraperitoneal Chemotherapy: A Pilot Study[J]. Mediators Of Inflammation, 2017.
        Sample Type: Serum
      8. Publication: Li T F, Li K, Wang C, et al. Harnessing The Cross-Talk Between Tumor Cells And Tumor-Associated Macrophages With A Nano-Drug For Modulation Of Glioblastoma Immune Microenvironment[J]. Journal of Controlled Release, 2017, 268: 128-146.
        Sample Type: culture supernatant
      9. Publication: Zheng S X, Weng Q Y, Wu W W, et al. Blood Purification Treatment Initiated At The Time Of Sepsis Diagnosis Effectively Attenuates Serum HMGB1 Upregulation And Improves Patient Prognosis[J]. Experimental and Therapeutic Medicine, 2017, 14: 3029-3035.
        Sample Type: Serum and urine
      10. Publication: Li T F, Li K, Zhang Q, et al. Dendritic cell-mediated delivery of doxorubicin-polyglycerol-nanodiamond composites elicits enhanced anti-cancer immune response in glioblastoma[J]. Biomaterials, 2018.
        Sample Type: Cell culture medium
      11. Publication: Wang F, Li X, Huang L, et al. High-Mobility Group Protein Box 1 is Upregulated in Children with Henoch-Schonlein Purpura[J]. Pediatric Allergy, Immunology, and Pulmonology, 2018, 31(2): 66-72.
        Sample Type: Human
      12. Publication: D’Angelo C, Reale M, Costantini E, et al. Profiling of Canonical and Non-Traditional Cytokine Levels in Interferon-β-Treated Relapsing–Remitting-Multiple Sclerosis Patients[J]. Frontiers in Immunology, 2018, 9.
        Sample Type: Human
      Show AllShorten
      • Show all (0)
      • Reviews (0)
      • Q&A (0)
      ... Show All Show Less
      MSDS for ELISA

      Browsing History

        People Also Bought