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Human IFN-γ(Interferon Gamma) ELISA Kit

  • Cat.No.:E-EL-H0108

  • Reactivity: Human

To Purchase E-EL-H0108

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IFN-γ in samples. No significant cross-reactivity or interference between Human IFN-γ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IFN-γ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IFN-γ. You can calculate the concentration of Human IFN-γ in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IFN-γ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IFN-γ were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 51.90 98.00 447.20 55.80 100.10 476.70
Standard deviation 3.20 4.80 16.10 2.80 5.40 24.30
CV (%) 6.17 4.90 3.60 5.02 5.39 5.10

Recovery

The recovery of Human IFN-γ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-107 101
EDTA plasma (n=8) 89-104 96
Cell culture media (n=8) 86-97 91

Linearity

Samples were spiked with high concentrations of Human IFN-γ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P01579
SynonymsIFNG, IFG, IFI, Type II Interferon
Research AreaCancer, Immunology, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Signal Transduction and Targeted Therapy (2021) IF: 18.187
    Differential immune responses in pregnant patients recovered from COVID-19

    DOI: 10.1038/s41392-021-00703-3

    PMID: 34326311

    Sample: blood
  2. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH (2020) IF: 11.161
    MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8 + T lymphocytes in human diffuse large B cell lymphoma

    DOI: 10.1186/s13046-020-01752-2

    PMID: 33168024

    Sample: Cell culture supernatant
  3. Acta Pharmaceutica Sinica B (2021) IF: 11.614
    Abrogation of HnRNP L enhances anti-PD-1 therapy efficacy via diminishing PD-L1 and promoting CD8+ T cell-mediated ferroptosis in castration-resistant prostate cancer

    DOI: 10.1016/j.apsb.2021.07.016

    Sample: PC3 cells,DU145 cells
  4. OncoImmunology (2022) IF: 8.1099
    Glucose metabolism inhibitor PFK-015 combined with immune checkpoint inhibitor is an effective treatment regimen in cancer

    DOI: 10.1080/2162402X.2022.2079182

    PMID: 35707221

    Sample: KYSE-70 cell,TE-11 cell
  5. Nanoscale (2021) IF: 7.79
    Rational design of a prodrug to inhibit self-inflammation for cancer treatment

    DOI: 10.1039/D1NR00132A

    PMID: 33710220

    Sample: Serum,Cell culture supernatant(dendritic cells,4T1
  6. Food & Function (2022) IF: 6.317
    Whole grain germinated brown rice regulates intestinal immune homeostasis and gastrointestinal hormones in type 2 diabetic patients—a randomized control trial

    DOI: 10.1039/D2FO00477A

    Sample: serum
  7. Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
    DNA Methyltransferase 3b Accelerates the Process of Atherosclerosis

    DOI: 10.1155/2022/5249367

    PMID: 35422896

    Sample: Tregs
  8. Nutrients (2022) IF: 6.706
    Thymoquinone Effect on Monocyte-Derived Macrophages, Cell-Surface Molecule Expression, and Phagocytosis

    DOI: 10.3390/nu14245240

    Sample: macrophage
  9. Cell Death Discovery (2021) IF: 5.241
    Glucose metabolism involved in PD-L1-mediated immune escape in the malignant kidney tumour microenvironment

    DOI: 10.1038/s41420-021-00401-7

    PMID: 33462221

    Sample: Cell culture supernatant
  10. Frontiers in Molecular Biosciences (2021) IF: 5.246
    Immunosuppression Induced by Glutamine Deprivation Occurs via Activating PD-L1 Transcription in Bladder Cancer

    DOI: 10.3389/fmolb.2021.687305

    PMID: 34805266

    Sample: T cells
  11. Food & Function (2022) IF: 5.396
    A randomised, double-blind, placebo-controlled trial of Bifidobacterium bifidum CCFM16 for manipulation of gut microbiota and relief from chronic constipation

    DOI: 10.1039/D1FO03896F

    Sample: serum
  12. JOURNAL OF CELLULAR PHYSIOLOGY (2020) IF: 5.546
    Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

    DOI: 10.1002/jcp.29713

    PMID: 32385929

    Sample: Cell culture supernatant
  13. WORLD JOURNAL OF GASTROENTEROLOGY (2021) IF: 5.742
    Serum soluble suppression of tumorigenicity 2 as a novel inflammatory marker predicts the severity of acute pancreatitis

    DOI: 10.3748/wjg.v27.i38.6489

    PMID: 34720537

    Sample: serum
  14. Clinical and Translational Allergy (2022) IF: 5.871
    MiR-29b regulates Th2 cell differentiation in asthma by targeting inducible B7-H3 and STAT3

    DOI: 10.1002/clt2.12114

    PMID: 35079347

    Sample: THP‐1 cell
  15. ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.119
    LncRNA MEG3 ameliorates NiO nanoparticles-induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway

    DOI: 10.1002/tox.23464

    PMID: 35006638

    Sample: A549 cell
  16. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2020) IF: 4.352
    S-adenosyl-L-methionine (SAMe) halts the autoimmune response in patients with primary biliary cholangitis (PBC) via antioxidant and S-glutathionylation processes in cholangiocytes

    DOI: 10.1016/j.bbadis.2020.165895

    PMID: 32681864

    Sample: Serum
  17. Scientific Reports (2021) IF: 4.38
    Diosmectite inhibits the interaction between SARS-CoV-2 and human enterocytes by trapping viral particles, thereby preventing NF-kappaB activation and CXCL10 secretion

    DOI: 10.1038/s41598-021-01217-2

    PMID: 34741071

    Sample: Caco-2 cells
  18. Journal of Oncology (2022) IF: 4.501
    IL-2 Combined with IL-15 Enhanced the Expression of NKG2D Receptor on Patient Autologous NK Cells to Inhibit Wilms’ Tumor via MAPK Signaling Pathway

    DOI: 10.1155/2022/4544773

    Sample: Wilms’ tumor (WT) tissue
  19. INFLAMMATION RESEARCH (2021) IF: 4.575
    Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis

    DOI: 10.1007/s00011-021-01459-w

    PMID: 33852061

    Sample: Serum,Bronchoalveolar Lavage Fluid
  20. MOLECULAR CANCER RESEARCH (2020) IF: 4.63
    Glutamine Deprivation Induces PD-L1 Expression via Activation of EGFR/ERK/c-Jun Signaling in Renal Cancer

    DOI: 10.1158/1541-7786.MCR-19-0517

    PMID: 31672701

    Sample: Cell culture supernatant
  21. INTERNATIONAL JOURNAL OF CARDIOLOGY (2016) IF: 4.638
    Decreased expression of programmed death 1 on peripheral blood lymphocytes disrupts immune homeostasis in peripartum cardiomyopathy

    DOI: 10.1016/j.ijcard.2016.08.232

    Sample: Serum
  22. Journal of Tissue Engineering and Regenerative Medicine (2016) IF: 4.71
    Platelet lysate supports the in vitro expansion of human periodontal ligament stem cells for cytotherapeutic use

    DOI: 10.1002/term.2124

    Sample: Cell culture supernatant
  23. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Bufalin enhances the killing efficacy of NK cells against hepatocellular carcinoma by inhibiting MICA shedding

    DOI: 10.1016/j.intimp.2021.108195

    PMID: 34678691

    Sample: SK-HEP-1 cell,HepG2 cell
  24. Scientific Reports (2022) IF: 4.996
    Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis

    DOI: 10.1038/s41598-022-21816-x

    Sample: serum
  25. PEDIATRIC PULMONOLOGY (2021) IF: 3.039
    Maternal psychiatric status and infant wheezing: The role of maternal hormones and cord blood cytokines

    DOI: 10.1002/ppul.25302

    PMID: 33587823

    Sample: cord blood(脐带血)
  26. KIDNEY & BLOOD PRESSURE RESEARCH (2017) IF: 3.104
    Association Between IFN-γ Gene Polymorphisms and IgA Nephropathy in a Chinese Han Population

    DOI: 10.1159/000473889

    Sample: Serum
  27. Oncology Letters (2022) IF: 3.111
    A role for serum cytokines and cell adhesion molecules in the non‑invasive diagnosis of colorectal cancer

    DOI: 10.3892/ol.2022.13443

    Sample: serum
  28. JOURNAL OF THE NEUROLOGICAL SCIENCES (2020) IF: 3.115
    Cytokine profile during pregnancy predicts relapses during pregnancy and postpartum in multiple sclerosis

    DOI: 10.1016/j.jns.2020.116811

    Sample: serum
  29. Biomed Research International (2022) IF: 3.246
    Regulatory Effects of Fyn on Trophoblast Cell Behaviors and Function

    DOI: 10.1155/2022/6006981

    PMID: 36330453

    Sample: HTR-8/SVneo cells,JEG-3 cells
  30. Bioengineered (2021) IF: 3.269
    Exosomes loaded with programmed death ligand-1 promote tumor growth by immunosuppression in osteosarcoma

    DOI: 10.1080/21655979.2021.1996509

    PMID: 34699324

    Sample: Jurkat cell
  31. NEUROLOGICAL SCIENCES (2021) IF: 3.307
    Evaluation of IL-10, IFN-γ, and thiol–disulfide homeostasis in patients with drug-resistant epilepsy

    DOI: 10.1007/s10072-021-05331-x

    Sample: Serum
  32. BMC Pulmonary Medicine (2021) IF: 3.317
    Systemic and local cytokine profile and risk factors for persistent allergic airway inflammation in patients sensitised to house dust mite allergens

    DOI: 10.1186/s12890-021-01798-8

    PMID: 34930201

    Sample: serum,nasal lavage
  33. OncoTargets and Therapy (2020) IF: 3.337
    MiRNA505/NET1 Axis Acts as a CD8+ T-TIL Regulator in Non-Small Cell Lung Cancer

    DOI: 10.2147/OTT.S265859

    Sample: Cell culture supernatant
  34. DISEASE MARKERS (2021) IF: 3.434
    Genetic Polymorphisms of IFNG, IFNGR1, and Androgen Receptor and Chronic Prostatitis/Chronic Pelvic Pain Syndrome in a Chinese Han Population

    DOI: 10.1155/2021/2898336

    PMID: 34646402

    Sample: serum
  35. CELL BIOLOGY INTERNATIONAL (2022) IF: 3.612
    The positive feedback loop of furin and TGFβ1 enhances the immune responses of Tregs to hepatocellular carcinoma cells and hepatitis B virus in vitro

    DOI: 10.1002/cbin.11806

    PMID: 35349767

    Sample: HepG2 cell
  36. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2022) IF: 3.657
    Can the Prognosis of COVID-19 Disease Be Determined by Fecal Markers and Cytokines?

    DOI: 10.1089/jir.2022.0098

    PMID: 36179036

    Sample: fecal
  37. Infectious Agents and Cancer (2023) IF: 3.698
    Inhibitory effect and mechanism of Lactobacillus crispatus on cervical precancerous cells Ect1/E6E7 and screening of early warning factors

    DOI: 10.1186/s13027-023-00483-1

    Sample: Ect1/E6E7 cell
  38. JOURNAL OF CELLULAR PHYSIOLOGY (2018) IF: 3.923
    Dehydroepiandrosterone stimulates inflammation and impairs ovarian functions of polycystic ovary syndrome

    DOI: 10.1002/jcp.27501

    PMID: 30580448

    Sample: Serum
  39. Annals of Translational Medicine (2022) IF: 3.932
    Possible causes of atherosclerosis: lncRNA COLCA1 induces oxidative stress in human coronary artery endothelial cells and impairs wound healing

    DOI: 10.21037/atm-22-507

    Sample: HCAECs
  40. Annals of Translational Medicine (2021) IF: 3.932
    Combination of oncolytic adenovirus ZD55 harboring TRAIL-IETD-MnSOD and cytokine-induced killer cells against lung cancer

    DOI: 10.21037/atm-21-4479

    PMID: 34790733

    Sample: serum,BEAS-2B cell
  41. PLoS Neglected Tropical Diseases (2016) IF: 3.948
    Clonorchis sinensis Co-infection Could Affect the Disease State and Treatment Response of HBV Patients

    DOI: 10.1371/journal.pntd.0004806

    Sample: Serum,Cell culture supernatant
  42. APOPTOSIS (2018) IF: 3.967
    MiRNA-126 expression inhibits IL-23R mediated TNF-α or IFN-γ production in fibroblast-like synoviocytes in a mice model of collagen-induced rheumatoid arthritis

    DOI: 10.1007/s10495-018-1474-7

    Sample: Serum
  43. IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2019) IF: 2.086
    In vitro impact of bisphenol A on maturation and function of monocyte-derived dendritic cells in patients with primary Sjögren’s syndrome

    DOI: 10.1080/08923973.2019.1706554

    Sample: PBMCs
  44. LUPUS (2020) IF: 2.251
    Cytokines, 25-OH vit D and disease activity in patients with juvenile-onset systemic lupus erythematosus:

    DOI: 10.1177/0961203320973068

    Sample: Plasma
  45. Biomed Research International (2020) IF: 2.276
    Blood Monocyte Phenotype Fingerprint of Stable Coronary Artery Disease: A Cross-Sectional Substudy of SMARTool Clinical Trial

    DOI: 10.1155/2020/8748934

    Sample: serum
  46. CARDIOVASCULAR PATHOLOGY (2017) IF: 2.359
    C reactive protein and enzymatically modified LDL cooperatively promote dendritic cell-mediated T cell activation

    DOI: 10.1016/j.carpath.2017.03.009

    Sample: Cell culture supernatant
  47. BIOLOGICAL TRACE ELEMENT RESEARCH (2019) IF: 2.431
    Study of Metal-Metal Interactions and Their Biomarkers Using an Intestinal Human Cell Line

    DOI: 10.1007/s12011-019-01831-2

    PMID: 31473895

    Sample: Cell lysate
  48. CLINICAL CARDIOLOGY (2016) IF: 2.431
    Expression of programmed cell death‐1 and its ligand B7 homolog 1 in peripheral blood lymphocytes from patients with peripartum cardiomyopathy

    DOI: 10.1002/clc.22661

    Sample: Serum
  49. INTERNATIONAL JOURNAL OF CLINICAL PRACTICE (2020) IF: 2.444
    The role of the kynurenine pathway and quinolinic acid in adolescent major depressive disorder

    DOI: 10.1111/ijcp.13739

    PMID: 32997876

    Sample: Serum
  50. Experimental and Therapeutic Medicine (2020) IF: 2.447
    Increased expression of IFN‑γ in preeclampsia impairs human trophoblast invasion via a SOCS1/JAK/STAT1 feedback loop

    DOI: 10.3892/etm.2020.9544

    Sample: Tissue homogenate
  51. American Journal of Rhinology & Allergy (2021) IF: 2.467
    Immunosuppressive Regulation of Dendritic Cells and T Cells in Allergic Rhinitis by Semaphorin 3A:

    DOI: 10.1177/19458924211005592

    Sample: Cell
  52. ACTA TROPICA (2018) IF: 2.509
    Assessment of chemokine and cytokine signatures in patients with dengue infection: A hospital-based study in Kolkata, India

    DOI: 10.1016/j.actatropica.2018.10.017

    PMID: 30395811

    Sample: Serum
  53. DERMATOLOGIC SURGERY (2020) IF: 2.567
    Efficacy of Intralesional Cryosurgery in the Treatment of Multiple Extragenital Cutaneous Warts: A Randomized Controlled Study

    DOI: 10.1097/DSS.0000000000002217

    Sample: serum
  54. TRANSFUSION AND APHERESIS SCIENCE (2022) IF: 2.596
    CFNAs of RBCs affect the release of inflammatory factors through the expression of CaMKIV in macrophages

    DOI: 10.1016/j.transci.2022.103494

    Sample: macrophages
  55. OncoTargets and Therapy (2018) IF: 2.656
    The elevated glutaminolysis of bladder cancer and T cells in a simulated tumor microenvironment contributes to the up-regulation of PD-L1 expression by interferon-γ

    DOI: 10.2147/OTT.S180505

    PMID: 30425515

    Sample: Cell culture supernatant
  56. LUPUS (2021) IF: 2.911
    Association of microRNA-125a with the clinical features, disease activity and inflammatory cytokines of juvenile-onset lupus patients:

    DOI: 10.1177/09612033211010328

    Sample: Plasma
  57. CYTOKINE (2020) IF: 2.952
    Involvement of the cytokine TWEAK in the pathogenesis of psoriasis vulgaris, pustular psoriasis, and erythrodermic psoriasis

    DOI: 10.1016/j.cyto.2020.155391

    PMID: 33302223

    Sample: serum
  58. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2020) IF: 2.985
    A novel human anti-TIGIT monoclonal antibody with excellent function in eliciting NK cell-mediated antitumor immunity

    DOI: 10.1016/j.bbrc.2020.12.013

    PMID: 33341068

    Sample: Cell culture supernatant
  59. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH (2020) IF: 1.287
    Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells:

    DOI: 10.1177/0300060520930853

    Sample: Cell culture supernatant
  60. Experimental and Therapeutic Medicine (2018) IF: 1.41
    Leukocyte depletion does not affect the in vitro healing ability of platelet rich plasma

    DOI: 10.3892/etm.2018.5887

    Sample: Cell culture supernatant
  61. ORL-Journal for Oto-Rhino-Laryngology Head and Neck Surgery (2021) IF: 1.538
    Clinical Efficacy and Possible Mechanism of Endoscopic Vidian Neurectomy for House Dust Mite-Sensitive Allergic Rhinitis

    DOI: 10.1159/000511711

    Sample: serum
  62. Dermatologic Therapy (2019) IF: 1.74
    Intralesional vitamin D3 versus intralesional purified protein derivative in treatment of multiple warts: A comparative clinical and immunological study

    DOI: 10.1111/dth.13034

    Sample: serum
  63. Journal of Thoracic Disease (2018) IF: 1.804
    Programmed cell death protein-1/programmed death-ligand 1 blockade enhances the antitumor efficacy of adoptive cell therapy against non-small cell lung cancer

    DOI: 10.21037/jtd.2018.10.111

    PMID: 30746216

    Sample: Cell culture supernatant
  64. Molecular Medicine Reports (2018) IF: 1.922
    The shortening telomere length of T lymphocytes maybe associated with hyper‑function in servere aplastic anemia

    DOI: 10.3892/mmr.2017.8014

    Sample: Cell culture supernatant
  65. BIOMARKERS (2018) IF: 1.976
    Influence of IL-6, IL-10, IFN-γ and TNF-α genetic variants on susceptibility to diabetic kidney disease in type 2 diabetes mellitus patients

    DOI: 10.1080/1354750X.2018.1501761

    Sample: plasma
  66. International Journal of Molecular and Cellular Medicine (2020)
    MicroRNAs as Immune Regulators of Inflammation in Children with Epilepsy

    DOI: 10.22088/IJMCM.BUMS.9.3.188

    Sample: plasma
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