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Human IFN-γ(Interferon Gamma) ELISA Kit

  • Cat.No.:E-EL-H0108

  • Reactivity: Human

To Purchase E-EL-H0108

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IFN-γ in samples. No significant cross-reactivity or interference between Human IFN-γ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IFN-γ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IFN-γ. You can calculate the concentration of Human IFN-γ in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IFN-γ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IFN-γ were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 51.90 98.00 447.20 55.80 100.10 476.70
Standard deviation 3.20 4.80 16.10 2.80 5.40 24.30
CV (%) 6.17 4.90 3.60 5.02 5.39 5.10

Recovery

The recovery of Human IFN-γ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-107 101
EDTA plasma (n=8) 89-104 96
Cell culture media (n=8) 86-97 91

Linearity

Samples were spiked with high concentrations of Human IFN-γ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P01579
SynonymsIFNG, IFG, IFI, Type II Interferon
Research AreaCancer,Immunology,Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. International Journal of Cardiology (2016) IF: 6.189
    Decreased Expression Of Programmed Death 1 On Peripheral Blood Lymphocytes Disrupts Immune Homeostasis In Peripartum Cardiomyopathy.

    DOI: 10.1016/j.ijcard.2016.08.232

    PMID: 27580218

    Sample: Serum
  2. Journal of Cellular Physiology (2020) IF: 5.546
    Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

    DOI: 10.1002/jcp.29713

    PMID: 32385929

    Sample: Cell culture supernatant
  3. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2020) IF: 4.352
    S-adenosyl-L-methionine (SAMe) halts the autoimmune response in patients with primary biliary cholangitis (PBC) via antioxidant and S-glutathionylation processes in cholangiocytes

    DOI: 10.1016/j.bbadis.2020.165895

    PMID: 32681864

    Sample: Serum
  4. Molecular Cancer Research (2020) IF: 4.63
    Glutamine Deprivation Induces PD-L1 Expression via Activation of EGFR/ERK/c-Jun Signaling in Renal Cancer

    DOI: 10.1158/1541-7786.MCR-19-0517

    PMID: 31672701

    Sample: Cell culture supernatant
  5. Clinical & Experimental Allergy (2020) IF: 4.217
    Relationship between immune parameters during a severe exacerbation in allergic asthmatic children and asthma outcomes in the following year

    DOI: 10.1111/cea.13570

    PMID: 31955476

    Sample: Plasma
  6. Apoptosis (2018) IF: 3.967
    miR-A-126 expression inhibits IL-23R mediated TNF-α or IFN-γ production in fibroblast-like synoviocytes in a mice model of collagen-induced rheumatoid arthritis

    DOI: 10.1007/s10495-018-1474-7

    PMID: 30167920

    Sample: Serum
  7. Journal of cellular physiology (2019) IF: 3.923
    Dehydroepiandrosterone stimulates inflammation and impairs ovarian functions of polycystic ovary syndrome

    DOI: 10.1002/jcp.27501

    PMID: 30580448

    Sample: Serum
  8. Kidney & Blood Pressure Research (2017) IF: 3.104
    Association Between IFN-γ Gene Polymorphisms And IgA Nephropathy In A Chinese Han Population.

    DOI: 10.1159/000473889

    PMID: 28391282

    Sample: Serum
  9. Journal of Tissue Engineering And Regenerative Medicine (2017) IF: 3.989
    Platelet Lysate Supports The In Vitro Expansion Of Human Periodontal Ligament Stem Cells For Cytotherapeutic Use.

    DOI: 10.1002/term.2124

    PMID: 26833905

    Sample: Cell culture supernatant
  10. Journal of Tissue Engineering And Regenerative Medicine (2016) IF: 3.989
    Platelet Lysate Supports The In Vitro Expansion Of Human Periodontal Ligament Stem Cells For Cytotherapeutic Use.

    DOI: 10.1002/term.2124

    PMID: 26833905

    Sample: Cell culture supernatant
  11. Plos Neglected Tropical Diseases (2016) IF: 3.834
    Clonorchis Sinensis Co-Infection Could Affect The Disease State And Treatment Response Of Hbv Patients.

    DOI: 10.1371/journal.pntd.0004806

    PMID: 27348302

    Sample: Serum,Cell culture supernatant
  12. International Journal of Clinical Practice (2020) IF: 2.444
    The role of the kynurenine pathway and quinolinic acid in adolescent major depressive disorder

    DOI: 10.1111/ijcp.13739

    PMID: 32997876

    Sample: Serum
  13. Journal of Zhejiang University. Science. B (2020) IF: 2.082
    Clinical characteristics of 34 COVID-19 patients admitted to intensive care unit in Hangzhou, China

    DOI: 10.1631/jzus.B2000174

    PMID: 32425003

    Sample: Serum,Plasma
  14. Biological Trace Element Research (2019) IF: 2.639
    Study of Metal-Metal Interactions and Their Biomarkers Using an Intestinal Human Cell Line

    DOI: 10.1007/s12011-019-01831-2

    PMID: 31473895

    Sample: Cell lysate
  15. Acta tropica (2019) IF: 2.509
    Assessment of chemokine and cytokine signatures in patients with dengue infection: A hospital-based study in Kolkata, India

    DOI: 10.1016/j.actatropica.2018.10.017

    PMID: 30395811

    Sample: Serum
  16. OncoTargets and Therapy (2018) IF: 2.66
    The elevated glutaminolysis of bladder cancer and T cells in a simulated tumor microenvironment contributes to the up-regulation of PD-l1 expression by interferon-γ

    DOI: 10.2147/OTT.S180505

    PMID: 30425515

    Sample: Cell culture supernatant
  17. Cardiovascular Pathology (2017) IF: 2.359
    C Reactive Protein And Enzymatically Modified LDL Cooperatively Promote Dendritic Cell-Mediated T Cell Activation.

    DOI: 10.1016/j.carpath.2017.03.009

    PMID: 28433861

    Sample: Cell culture supernatant
  18. Clinical Cardiology (2016) IF: 2.757
    Expression Of Programmed Cell Death-1 And Its Ligand B7 Homolog 1 In Peripheral Blood Lymphocytes From Patients With Peripartum Cardiomyopathy.

    DOI: 10.1002/clc.22661

    PMID: 28026044

    Sample: Serum
  19. Journal of International Medical Research (2020) IF: 1.287
    Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells

    DOI: 10.1177/0300060520930853

    PMID: 32663414

    Sample: Cell culture supernatant
  20. Journal of thoracic disease (2018) IF: 1.804
    Programmed cell death protein-1/programmed death-ligand 1 blockade enhances the antitumor efficacy of adoptive cell therapy against non-small cell lung cancer

    DOI: 10.21037/jtd.2018.10.111

    PMID: 30746216

    Sample: Cell culture supernatant
  21. Molecular Medicine Reports (2018) IF: 1.692
    The Shortening Telomere Length Of T Lymphocytes Maybe Associated With HyperFunction In Servere Aplastic Anemia.

    DOI: 10.3892/mmr.2017.8014

    PMID: 29115638

  22. International Journal of Clinical and Experimental Pathology (2017) IF: 1.706
    Inflammatory Characteristics In Different Types Of Nonallergic Rhinitis.
    Sample: Cell culture supernatant
  23. International Electronic Journal of Medicine (2020)
    Hematological Changes, Serum Interferon Gamma and Interleukin-4 Alterations in Normal Pregnancy and Preeclampsia

    DOI: 10.34172/iejm.2020.08

    PMID: 99999999

    Sample: Serum
  24. Medico Legal Update (2020)
    High Levels of Tumor Necrosis Factor-Alpha and Interferon-Gamma in Patients with Gingival Recession

    DOI: 10.37506/v20/i1/2020/mlu/194424

    PMID: 99999999

    Sample: Serum
  25. Journal of Physics: Conference Series (2019)
    Correlation between the percentage of memory T cells and IFN-γ level in patients with systemic lupus erythematous

    DOI: 10.1088/1742-6596/1246/1/012015/meta

    PMID: 99999999

    Sample: Serum
  26. Experimental and Therapeutic Medicine (2018)
    Leukocyte Depletion Does Not Affect The In Vitro Healing Ability Of Platelet Rich Plasma.

    DOI: 10.3892/etm.2018.5887

    PMID: 29556269

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