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Human IGF-1(Insulin-like Growth Factor 1) ELISA Kit

  • Cat.No.:E-EL-H0086

  • Reactivity: Human

To Purchase E-EL-H0086

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 1.56-100 ng/mL
Sensitivity 0.94 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IGF-1 in samples. No significant cross-reactivity or interference between Human IGF-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IGF-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGF-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IGF-1. You can calculate the concentration of Human IGF-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IGF-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IGF-1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 5.30 11.80 48.60 5.60 10.90 47.70
Standard deviation 0.30 0.50 1.90 0.30 0.50 1.80
CV (%) 5.66 4.24 3.91 5.36 4.59 3.77


The recovery of Human IGF-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-106 100
EDTA plasma (n=8) 92-108 99
Cell culture media (n=8) 86-99 92


Samples were spiked with high concentrations of Human IGF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P05019
SynonymsIGF1, IGFI, IGF-I, IGF1A, IGF-IA, IGF-IB, MGF, Somatomedin C
Research AreaCancer, Developmental biology, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. CANCER LETTERS (2021) IF: 8.679
    LncCCLM inhibits lymphatic metastasis of cervical cancer by promoting STAU1-mediated IGF-1 mRNA degradation

    DOI: 10.1016/j.canlet.2021.07.005

    PMID: 34273467

    Sample: CC cells
  2. Stem Cells International (2021) IF: 5.443
    Mechanical Stress-Induced IGF-1 Facilitates col-I and col-III Synthesis via the IGF-1R/AKT/mTORC1 Signaling Pathway

    DOI: 10.1155/2021/5553676

    PMID: 34912459

    Sample: LFC
  3. Cell Death & Disease (2019) IF: 5.959
    Insulin-like growth factor-1 attenuates oxidative stress-induced hepatocyte premature senescence in liver fibrogenesis via regulating nuclear p53–progerin interaction

    DOI: 10.1038/s41419-019-1670-6

    Sample: plasm
    Comparison of Serum VEGF, IGF-1, and HIF-1α Levels in Children with Autism Spectrum Disorder and Healthy Controls

    DOI: 10.1007/s10803-020-04820-w

    PMID: 33389301

    Sample: Serum
  5. Scientific Reports (2021) IF: 4.38
    Obesity-related proteins score as a potential marker of breast cancer risk

    DOI: 10.1038/s41598-021-87583-3

    PMID: 33859244

    Sample: Plasma
  6. Journal of Hepatocellular Carcinoma (2020) IF: 4.655
    Prognostic Significance of Serum Insulin-Like Growth Factor-1 in Hepatocellular Cancer Patients: A Validation Study

    DOI: 10.2147/JHC.S258930

    Sample: serum
  7. Aging-US (2020) IF: 4.831
    MiR-483 induces senescence of human adipose-derived mesenchymal stem cells through IGF1 inhibition

    DOI: 10.18632/aging.103818

    PMID: 32805717

    Sample: Cell sample
    CTCF variant begets to short stature by down-regulation of IGF-1

    DOI: 10.1530/JME-22-0193

    Sample: HEK-293T cell,LO2 cell,immortalized lymphocyte cel
  9. NEUROSCIENCE LETTERS (2022) IF: 3.046
    Role of IGF-1 in neuroinflammation and cognition deficits induced by sleep deprivation

    DOI: 10.1016/j.neulet.2022.136575

    PMID: 35276231

    Sample: Plasma
    Ramadan diurnal intermittent fasting modulates SOD2, TFAM, Nrf2, and sirtuins (SIRT1, SIRT3) gene expressions in subjects with overweight and obesity

    DOI: 10.1016/j.diabres.2019.107801

    Sample: serum
    Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals

    DOI: 10.1016/j.diabres.2019.05.023

    PMID: 31150725

    Sample: Serum
  12. Frontiers in Nutrition (2020) IF: 3.365
    A Novel 7-Days Prolonged Dietary Deprivation Regimen Improves ALT and UA After 3–6 Months Refeeding, Indicating Therapeutic Potential

    DOI: 10.3389/fnut.2020.00050

    Sample: serum
  13. GROWTH HORMONE & IGF RESEARCH (2022) IF: 2.372
    In missed abortion the decrease of IGF-1 down-regulates PI3K/AKT signaling pathway reducing the secretion of progesterone and β-hCG

    DOI: 10.1016/j.ghir.2022.101479

    PMID: 35752133

    Sample: serum
    The rhenium(I)-diselenoether anticancer drug targets ROS, TGF-β1, VEGF-A, and IGF-1 in an in vitro experimental model of triple-negative breast cancers

    DOI: 10.1007/s10637-019-00727-1

    PMID: 30632005

    Sample: Cell culture medium
  15. ACTA DIABETOLOGICA (2019) IF: 2.996
    polymorphisms with diabetic retinopathy in Pakistani population

    DOI: 10.1007/s00592-019-01407-5

    Sample: Serum
  16. AURIS NASUS LARYNX (2020) IF: 1.436
    Association between adenotonsillar hypertrophy and leptin, ghrelin and IGF-1 levels in children

    DOI: 10.1016/j.anl.2020.08.002

    Sample: serum
  17. Endokrynologia Polska (2021) IF: 1.582
    New insights into the metabolic-bone crosstalk in active acromegaly

    DOI: 10.5603/EP.a2021.0028

    Sample: serum
    Effect of KiFAY on Performance, Insulin-like Growth Factor-1, and Thyroid Hormones in Broilers

    DOI: 10.5713/ajas.16.0188

    Sample: Serum
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