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Human IL-12(Interleukin 12) ELISA Kit

  • Cat.No.:E-EL-H0150

  • Reactivity: Human

To Purchase E-EL-H0150

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-12 in samples. No significant cross-reactivity or interference between Human IL-12 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-12 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-12. You can calculate the concentration of Human IL-12 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-12 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-12 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 48.00 105.40 420.80 43.40 112.70 443.80
Standard deviation 3.20 4.70 17.30 2.80 5.20 23.50
CV (%) 6.67 4.46 4.11 6.45 4.61 5.30


The recovery of Human IL-12 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-99 92
EDTA plasma (n=8) 87-100 92
Cell culture media (n=8) 85-100 92


Samples were spiked with high concentrations of Human IL-12 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P29459  P29460
SynonymsIL12, IL-12/p70, IL12 p70
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Oxidative Medicine and Cellular Longevity (2017) IF: 4.593
    Ros-Dependent Activation Of Autophagy Through The PI3K/Akt/mTOR Pathway Is Induced By Hydroxysafflor Yellow A-Sonodynamic Therapy In THP-1 Macrophages.

    DOI: 10.1155/2017/8519169

    PMID: 28191279

    Sample: Cell culture supernatant
  2. Molecular immunology (2019) IF: 3.188
    IL-16 regulates macrophage polarization as a target gene of miR-145-3p

    DOI: 10.1016/j.molimm.2018.12.027

    PMID: 30634164

    Sample: Cell lysate
  3. Acta tropica (2019) IF: 2.509
    Assessment of chemokine and cytokine signatures in patients with dengue infection: A hospital-based study in Kolkata, India

    DOI: 10.1016/j.actatropica.2018.10.017

    PMID: 30395811

    Sample: Serum
  4. Apmis (2019) IF: 2.026
    Autoantibodies to a Specific Peptide Epitope of Human Hsp60 (ATVLA) With Homology to Helicobacter Pylori HspB in H. Pylori-Infected Patients

    DOI: 10.1111/apm.12925

    PMID: 30746790

    Sample: Cell culture supernatant
  5. Cardiovascular Pathology (2017) IF: 2.359
    C Reactive Protein And Enzymatically Modified LDL Cooperatively Promote Dendritic Cell-Mediated T Cell Activation.

    DOI: 10.1016/j.carpath.2017.03.009

    PMID: 28433861

    Sample: Cell culture supernatant
  6. Viral Immunology (2016) IF: 1.432
    Α-Mannosidase I Protein Expression In Peripheral Blood Mononuclear Cells Is Upregulated During Hepatitis B Virus Infection.

    DOI: 10.1089/vim.2015.0079

    PMID: 26569026

    Sample: Serum
  7. International Journal of Research in Science and Technology (2020)

    DOI: 10.37648/ijrst.v10i02.003

    Sample: Serum
  8. Journal of THI-QAR SCIENCE (2017)
    The Relationship between Cytokines and Toxoplasma Infection in Heart Disease Patients.
    Sample: Serum
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