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Human IL-13(Interleukin 13) ELISA Kit

  • Cat.No.:E-EL-H0104

  • Reactivity: Human

To Purchase E-EL-H0104

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-13 in samples. No significant cross-reactivity or interference between Human IL-13 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-13 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-13. You can calculate the concentration of Human IL-13 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-13 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-13 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 51.30 137.59 357.48 50.92 126.69 348.07
Standard deviation 2.99 8.02 18.16 3.08 7.20 11.14
CV (%) 5.83 5.83 5.08 6.05 5.68 3.20

Recovery

The recovery of Human IL-13 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 83-99 90
EDTA plasma (n=8) 86-102 94
Cell culture media (n=8) 88-100 93

Linearity

Samples were spiked with high concentrations of Human IL-13 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P35225
SynonymsIL13, ALRH, BHR1, P600
Research AreaCancer, Cardiovascular, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Nature Communications (2021) IF: 14.919
    Specific gut microbiome signatures and the associated pro-inflamatory functions are linked to pediatric allergy and acquisition of immune tolerance

    DOI: 10.1038/s41467-021-26266-z

    PMID: 34645820

    Sample: CD4+ cell
  2. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2021) IF: 5.279
    Reducing the Allergenicity of α-Lactalbumin after Lipid Peroxidation

    DOI: 10.1021/acs.jafc.1c00559

    PMID: 33974424

    Sample: Cell culture supernatant
  3. WORLD JOURNAL OF GASTROENTEROLOGY (2021) IF: 5.742
    Serum soluble suppression of tumorigenicity 2 as a novel inflammatory marker predicts the severity of acute pancreatitis

    DOI: 10.3748/wjg.v27.i38.6489

    PMID: 34720537

    Sample: serum
  4. INFLAMMATION RESEARCH (2021) IF: 4.575
    Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis

    DOI: 10.1007/s00011-021-01459-w

    PMID: 33852061

    Sample: Serum,Bronchoalveolar Lavage Fluid
  5. PEDIATRIC PULMONOLOGY (2021) IF: 3.039
    Maternal psychiatric status and infant wheezing: The role of maternal hormones and cord blood cytokines

    DOI: 10.1002/ppul.25302

    PMID: 33587823

    Sample: cord blood(脐带血)
  6. BMC Pulmonary Medicine (2021) IF: 3.317
    Systemic and local cytokine profile and risk factors for persistent allergic airway inflammation in patients sensitised to house dust mite allergens

    DOI: 10.1186/s12890-021-01798-8

    PMID: 34930201

    Sample: serum,nasal lavage
  7. BMC Pulmonary Medicine (2021) IF: 3.317
    Role of IL-22 in persistent allergic airway diseases caused by house dust mite: a pilot study

    DOI: 10.1186/s12890-021-01410-z

    PMID: 33478443

    Sample: Serum,Nasal Lavage Fluid
  8. HUMAN IMMUNOLOGY (2022) IF: 2.85
    The function of IL-33/ST2 signaling axis in treg cells activating fibrosis in IgG4-related disease

    DOI: 10.1016/j.humimm.2022.01.009

    PMID: 35067387

    Sample: Serum
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