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Human IL-18(Interleukin 18) ELISA Kit

Citations(38)Uniprot : Q14116

Cat.No.:E-EL-H0253
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human IL-18 in samples. No significant cross-reactivity or interference between Human IL-18 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human IL-18 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-18. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-18, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-18. You can calculate the concentration of Human IL-18 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-18 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-18 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	54.60	160.50	353.50	56.70	176.10	373.50
Standard deviation	3.30	7.50	17.00	3.90	7.70	13.40
CV (%)	6.04	4.67	4.81	6.88	4.37	3.59

Recovery

The recovery of Human IL-18 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	94-109	100
EDTA plasma (n=8)	89-101	96
Cell culture media (n=8)	85-96	91

Linearity

Samples were spiked with high concentrations of Human IL-18 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q14116
Synonyms	IL18, IGIF, IL-1g, IL-1F4, Interferon-gamma-inducing factor
Research Area	Cancer, Metabolism, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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Cell Death & Disease (2021) IF: 8.4689

Histone deacetylase 2 regulates ULK1 mediated pyroptosis during acute liver failure by the K68 acetylation site

DOI: 10.1038/s41419-020-03317-9
PMID: 33431796	Sample: Serum,Cell culture supernatant

Cellular and Molecular Gastroenterology and Hepatology (2022) IF: 8.7970

Klebsiella pneumoniae induces inflammatory bowel disease through caspase-11-mediated IL-18 in the gut epithelial cells

DOI: 10.1016/j.jcmgh.2022.11.005
	Sample: human colon organoid

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 7.129

Autophagy promotes GSDME-mediated pyroptosis via intrinsic and extrinsic apoptotic pathways in cobalt chloride-induced hypoxia reoxygenation-acute kidney injury

DOI: 10.1016/j.ecoenv.2022.113881
PMID: 35863214	Sample: HK-2 cell

FOOD RESEARCH INTERNATIONAL (2022) IF: 7.425

N-3 polyunsaturated fatty acids protect esophageal epithelial cells from acid exposure

DOI: 10.1016/j.foodres.2022.111943
PMID: 36461199	Sample: Het-1A cell

Cells (2022) IF: 7.666

Hypoxia-Induced HIF-1α Expression Promotes Neurogenic Bladder Fibrosis via EMT and Pyroptosis

DOI: 10.3390/cells11233836

Frontiers in Cell and Developmental Biology (2021) IF: 6.684

Autophagy-Based Unconventional Secretory for AIM2 Inflammasome Drives DNA Damage Resistance During Intervertebral Disc Degeneration

DOI: 10.3389/fcell.2021.672847
PMID: 34239872	Sample: NP cells

Nutrients (2022) IF: 6.706

CCN1/Integrin α5β1 Instigates Free Fatty Acid-Induced Hepatocyte Lipid Accumulation and Pyroptosis through NLRP3 Inflammasome Activation

DOI: 10.3390/nu14183871
	Sample: LO2 hepatocytes

Journal of Inflammation Research (2022) IF: 6.922

Long Noncoding RNA MIAT Regulates Hyperosmotic Stress-Induced Corneal Epithelial Cell Injury via Inhibiting the Caspase-1-Dependent Pyroptosis and Apoptosis in Dry Eye Disease

DOI: 10.2147/JIR.S361541
PMID: 35676970	Sample: Human corneal epithelial cell (HCECs)

OSTEOARTHRITIS AND CARTILAGE (2018) IF: 5.454

Associations between knee structural measures, circulating inflammatory factors and MMP13 in patients with knee osteoarthritis

DOI: 10.1016/j.joca.2018.05.003
	Sample: Serum

AIDS (2015) IF: 5.554

Switch to maraviroc/raltegravir dual therapy leads to an unfavorable immune profile with low-level HIV viremia

DOI: 10.1097/QAD.0000000000000626
	Sample: plasma

MOLECULAR NEUROBIOLOGY (2022) IF: 5.682

Acrylamide Induces Neurotoxicity in SH-SY5Y Cells via NLRP3-mediated Pyroptosis

DOI: 10.1007/s12035-022-03098-6

American Journal of Translational Research (2019) IF: 4.06

HMGB1-induced endothelial cell pyroptosis is involved in systemic inflammatory response syndrome following radiofrequency ablation of hepatic hemangiomas


PMID: 31934300	Sample: serum,HUVECs

JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY (2021) IF: 4.158

Autophagy blockage promotes the pyroptosis of ox-LDL-treated macrophages by modulating the p62/Nrf2/ARE axis

DOI: 10.1007/s13105-021-00811-2
PMID: 33886081	Sample: Cell culture supernatant

CELLULAR MICROBIOLOGY (2019) IF: 4.288

Fatty acylCoA synthetase FadD13 regulates proinflammatory cytokine secretion dependent on the NF‐κB signalling pathway by binding to eEF1A1

DOI: 10.1111/cmi.13090
	Sample: Cell culture supernatant

JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36

Preservation of mitochondrial homeostasis is responsible for the ameliorative effects of Suhuang antitussive capsule on non-resolving inflammation via inhibition of NF-κB signaling and NLRP3 inflammasome activation

DOI: 10.1016/j.jep.2021.113827
PMID: 33460751	Sample: Cell culture supernatant(THP-1 cells)

CELL BIOLOGY INTERNATIONAL (2023) IF: 4.473

TFEB attenuates hyperglycemia-induced retinal capillary endothelial cells injury via autophagy regulation

DOI: 10.1002/cbin.12002
	Sample: human retinal capillary endothelial cells (HRCECs)

Toxins (2021) IF: 4.546

Biomarkers of Uremic Cardiotoxicity

DOI: 10.3390/toxins13090639
PMID: 34564643	Sample: serum

Frontiers in Immunology (2019) IF: 4.716

Mechanistic Insight Into the Activation of the NLRP3 Inflammasome by Neisseria gonorrhoeae in Macrophages

DOI: 10.3389/fimmu.2019.01815
	Sample: THP-1 cells

Journal of Immunology Research (2021) IF: 4.818

Moesin Is a Novel Biomarker of Endothelial Injury in Sepsis

DOI: 10.1155/2021/6695679
PMID: 33628853	Sample: Cell culture supernatant(HMECs)

Aging-US (2020) IF: 4.831

Electrical stimulation inhibits Val-boroPro-induced pyroptosis in THP-1 macrophages via sirtuin3 activation to promote autophagy and inhibit ROS generation

DOI: 10.18632/aging.103038
	Sample: THP-1 cell

INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932

ESAT-6 impedes IL-18 mediated phagosome lysosome fusion via microRNA-30a upon Calcimycin treatment in mycobacteria infected macrophages

DOI: 10.1016/j.intimp.2021.108319
PMID: 34740079	Sample: THP-1 cells

INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932

Chlorogenic acid attenuates inflammation in LPS-induced Human gingival fibroblasts via CysLT1R/Nrf2/NLRP3 signaling

DOI: 10.1016/j.intimp.2022.108706
PMID: 35313270	Sample: Human gingival fibroblasts (HGFs)

NEUROCHEMICAL RESEARCH (2020) IF: 3.038

MiR-1202 Exerts Neuroprotective Effects on OGD/R Induced Inflammation in HM Cell by Negatively Regulating Rab1a Involved in TLR4/NF-κB Signaling Pathway

DOI: 10.1007/s11064-020-02991-7
PMID: 32124161	Sample: Cell culture medium

INFLAMMATION RESEARCH (2019) IF: 3.061

TNF-α/calreticulin dual signaling induced NLRP3 inflammasome activation associated with HuR nucleocytoplasmic shuttling in rheumatoid arthritis

DOI: 10.1007/s00011-019-01244-w
	Sample: rheumatoid arthritis (RA) fibroblast-like synovioc

Bioengineered (2021) IF: 3.269

Knock-down of microRNA miR-556-5p increases cisplatin-sensitivity in non-small cell lung cancer (NSCLC) via activating NLR family pyrin domain containing 3 (NLRP3)-mediated pyroptotic cell death

DOI: 10.1080/21655979.2021.1971502
PMID: 34488537	Sample: A549 cell,H1299 cell

FISH & SHELLFISH IMMUNOLOGY (2019) IF: 3.298

The first cloned echinoderm tumor necrosis factor receptor from Holothuria leucospilota: Molecular characterization and functional analysis

DOI: 10.1016/j.fsi.2019.08.008
PMID: 31394160	Sample: Cell culture supernatant

Biomed Research International (2020) IF: 3.411

CD147 Aggravated Inflammatory Bowel Disease by Triggering NF-κB-Mediated Pyroptosis

DOI: 10.1155/2020/5341247
	Sample: Serum

EXPERIMENTAL EYE RESEARCH (2021) IF: 3.467

Wedelolactone ameliorates Pseudomonas aeruginosa-induced inflammation and corneal injury by suppressing caspase-4/5/11/GSDMD-mediated non-canonical pyroptosis

DOI: 10.1016/j.exer.2021.108750
PMID: 34481822	Sample: piHCKs

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2021) IF: 3.652

Proanthocyanidins attenuate the high glucose-induced damage of retinal pigment epithelial cells by attenuating oxidative stress and inhibiting activation of the NLRP3 inflammasome

DOI: 10.1002/jbt.22845
PMID: 34338401	Sample: ARPE-19 cell

Annals of Translational Medicine (2021) IF: 3.932

Effect and mechanism of phospholipid scramblase 4 (PLSCR4) on lipopolysaccharide (LPS)-induced injury to human pulmonary microvascular endothelial cells

DOI: 10.21037/atm-20-7983
PMID: 33569461	Sample: HPMECs

JOURNAL OF MEDICAL VIROLOGY (2020) IF: 2.021

Evaluation of alpha defensin, IL‐1 receptor antagonist, and IL‐18 levels in COVID‐19 patients with macrophage activation syndrome and acute respiratory distress syndrome

DOI: 10.1002/jmv.26589
	Sample: Plasma

JOURNAL OF ORAL PATHOLOGY & MEDICINE (2019) IF: 2.03

Assessment of Interleukin‐18 gene polymorphism and serum levels in oral lichen planus in an Indian population

DOI: 10.1111/jop.12830
	Sample: serum

Innate Immunity (2019) IF: 2.173

Functional characterisation of Holothuria leucospilota Fas-associated death domain in the innate immune-related signalling pathways:

DOI: 10.1177/1753425919877680
	Sample: Cell culture supernatant

PeerJ (2019) IF: 2.353

The role of serum and urinary biomarkers in the diagnosis of early diabetic nephropathy in patients with type 2 diabetes

DOI: 10.7717/peerj.7079
	Sample: urinary,serum

BMC Molecular and Cell Biology (2022) IF: 2.813

Ceramide induces pyroptosis through TXNIP/NLRP3/GSDMD pathway in HUVECs

DOI: 10.1186/s12860-022-00459-w
	Sample: Human Umbilical Vein Endothelial Cells (HUVECs)

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2020) IF: 2.985

Metformin alleviates high glucose-induced ER stress and inflammation by inhibiting the interaction between caveolin1 and AMPKα in rat astrocytes

DOI: 10.1016/j.bbrc.2020.10.075
PMID: 33143871	Sample: Cell culture supernatant

SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION (2019) IF: 1.38

Endothelial pyroptosis underlies systemic inflammatory response following radiofrequency ablation of hepatic hemangiomas

DOI: 10.1080/00365513.2019.1689428
PMID: 31710248	Sample: Serum,Cell culture supernatant

International Journal of Ophthalmology (2021) IF: 1.779

Effects of quercetin on diabetic retinopathy and its association with NLRP3 inflammasome and autophagy

DOI: 10.18240/ijo.2021.01.06
PMID: 33469482	Sample: Cell culture supernatant

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