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Human IL-18(Interleukin 18) ELISA Kit

  • Cat.No.:E-EL-H0253

  • Reactivity: Human

To Purchase E-EL-H0253

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-18 in samples. No significant cross-reactivity or interference between Human IL-18 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-18 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-18. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-18, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-18. You can calculate the concentration of Human IL-18 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-18 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-18 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 54.60 160.50 353.50 56.70 176.10 373.50
Standard deviation 3.30 7.50 17.00 3.90 7.70 13.40
CV (%) 6.04 4.67 4.81 6.88 4.37 3.59

Recovery

The recovery of Human IL-18 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-109 100
EDTA plasma (n=8) 89-101 96
Cell culture media (n=8) 85-96 91

Linearity

Samples were spiked with high concentrations of Human IL-18 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: Q14116
SynonymsIL18, IGIF, IL-1g, IL-1F4, Interferon-gamma-inducing factor
Research AreaImmunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Osteoarthritis and cartilage (2018) IF: 5.454
    Associations between knee structural measures, circulating inflammatory factors and MMP13 in patients with knee osteoarthritis.

    DOI: 10.1016/j.joca.2018.05.003

    PMID: 29753949

    Sample: Serum
  2. Neurochemical Research (2020) IF: 3.038
    miR-1202 Exerts Neuroprotective Effects on OGD/R Induced Inflammation in HM Cell by Negatively Regulating Rab1a Involved in TLR4/NF-κB Signaling Pathway

    DOI: 10.1007/s11064-020-02991-7

    PMID: 32124161

    Sample: Cell culture medium
  3. Cellular microbiology (2019) IF: 3.43
    Fatty acylCoA synthetase FadD13 regulates proinflammatory cytokine secretion dependent on the NF-κB signalling pathway by binding to eEF1A1

    DOI: 10.1111/cmi.13090

    PMID: 31364251

    Sample: Cell culture supernatant
  4. Fish & Shellfish Immunology (2019) IF: 3.298
    The first cloned echinoderm tumor necrosis factor receptor from Holothuria leucospilota: Molecular characterization and functional analysis

    DOI: 10.1016/j.fsi.2019.08.008

    PMID: 31394160

    Sample: Cell culture supernatant
  5. BioMed Research International (2020) IF: 2.276
    CD147 Aggravated Inflammatory Bowel Disease by Triggering NF- κ B-Mediated Pyroptosis

    DOI: 10.1155/2020/5341247

    PMID: 32714980

    Sample: Serum
  6. Innate Immunity (2020) IF: 2.298
    Functional characterisation of Holothuria leucospilota Fas-associated death domain in the innate immune-related signalling pathways

    DOI: 10.1177/1753425919877680

    PMID: 31550955

    Sample: Cell culture supernatant
  7. Scandinavian Journal of Clinical and Laboratory Investigation (2019) IF: 1.475
    Endothelial pyroptosis underlies systemic inflammatory response following radiofrequency ablation of hepatic hemangiomas

    DOI: 10.1080/00365513.2019.1689428

    PMID: 31710248

    Sample: Serum,Cell culture supernatant
  8. Egyptian Journal of Dermatology and Venerology (2020)
    Evaluation of interleukin-18 and soluble interleukin-2 receptor serum levels in patients with alopecia areata: an Egyptian study

    DOI: 10.4103/ejdv.ejdv_34_19

    PMID: 99999999

    Sample: Serum
  9. The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy (2018)
    Overview of Serum Interleukin-18 (IL-18) Levels in Liver Cirrhosis Patients and Their Correlation to Hepatic Encephalopathy

    DOI: 10.24871/192201867-73

    PMID: 99999999

    Sample: Serum
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