Current Position:Home>>Kits>>ELISA Kits>> Human IL-18(Interleukin 18) ELISA Kit
Cat.No.:E-EL-H0253
Welcome to order from local distributors.
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 15.63-1000 pg/mL |
Sensitivity | 9.38 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human IL-18 in samples. No significant cross-reactivity or interference between Human IL-18 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human IL-18 concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-18 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-18 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 54.60 | 160.50 | 353.50 | 56.70 | 176.10 | 373.50 |
Standard deviation | 3.30 | 7.50 | 17.00 | 3.90 | 7.70 | 13.40 |
CV (%) | 6.04 | 4.67 | 4.81 | 6.88 | 4.37 | 3.59 |
The recovery of Human IL-18 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 94-109 | 100 |
EDTA plasma (n=8) | 89-101 | 96 |
Cell culture media (n=8) | 85-96 | 91 |
Samples were spiked with high concentrations of Human IL-18 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
Q14116
|
Synonyms | IL18, IGIF, IL-1g, IL-1F4, Interferon-gamma-inducing factor |
Research Area | Cancer, Metabolism, Immunology |
![]() |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
![]() |
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
![]() |
3. Aspirate and wash the plate for 3 times |
![]() |
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
![]() |
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
![]() |
6. Add 50μL Stop Solution |
![]() |
7. Read the plate at 450nm immediately. Calculation of the results |
From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!
PMID: 33431796 |
Sample: Serum,Cell culture supernatant |
Sample: human colon organoid |
PMID: 35863214 |
Sample: HK-2 cell |
PMID: 36461199 |
Sample: Het-1A cell |
PMID: 34239872 |
Sample: NP cells |
DOI: 10.3390/nu14183871 |
|
Sample: LO2 hepatocytes |
DOI: 10.2147/JIR.S361541 |
|
PMID: 35676970 |
Sample: Human corneal epithelial cell (HCECs) |
Sample: Serum |
Sample: plasma |
PMID: 31934300 |
Sample: serum,HUVECs |
PMID: 33886081 |
Sample: Cell culture supernatant |
DOI: 10.1111/cmi.13090 |
|
Sample: Cell culture supernatant |
PMID: 33460751 |
Sample: Cell culture supernatant(THP-1 cells) |
DOI: 10.1002/cbin.12002 |
|
Sample: human retinal capillary endothelial cells (HRCECs) |
PMID: 34564643 |
Sample: serum |
Sample: THP-1 cells |
DOI: 10.1155/2021/6695679 |
|
PMID: 33628853 |
Sample: Cell culture supernatant(HMECs) |
Sample: THP-1 cell |
PMID: 34740079 |
Sample: THP-1 cells |
PMID: 35313270 |
Sample: Human gingival fibroblasts (HGFs) |
PMID: 32124161 |
Sample: Cell culture medium |
Sample: rheumatoid arthritis (RA) fibroblast-like synovioc |
PMID: 34488537 |
Sample: A549 cell,H1299 cell |
PMID: 31394160 |
Sample: Cell culture supernatant |
DOI: 10.1155/2020/5341247 |
|
Sample: Serum |
PMID: 34481822 |
Sample: piHCKs |
DOI: 10.1002/jbt.22845 |
|
PMID: 34338401 |
Sample: ARPE-19 cell |
DOI: 10.21037/atm-20-7983 |
|
PMID: 33569461 |
Sample: HPMECs |
DOI: 10.1002/jmv.26589 |
|
Sample: Plasma |
DOI: 10.1111/jop.12830 |
|
Sample: serum |
Sample: Cell culture supernatant |
DOI: 10.7717/peerj.7079 |
|
Sample: urinary,serum |
Sample: Human Umbilical Vein Endothelial Cells (HUVECs) |
PMID: 33143871 |
Sample: Cell culture supernatant |
PMID: 31710248 |
Sample: Serum,Cell culture supernatant |
PMID: 33469482 |
Sample: Cell culture supernatant |