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Human IL-1α(Interleukin 1 Alpha) ELISA Kit

  • Cat.No.:E-EL-H0088

  • Reactivity: Human

To Purchase E-EL-H0088

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 3.91-250 pg/mL
Sensitivity 2.35 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-1α in samples. No significant cross-reactivity or interference between Human IL-1α and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-1α concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-1α. You can calculate the concentration of Human IL-1α in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-1α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-1α were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 11.90 22.70 113.00 11.60 22.40 105.70
Standard deviation 0.70 1.20 3.60 0.80 1.30 3.70
CV (%) 5.88 5.29 3.19 6.90 5.80 3.50


The recovery of Human IL-1α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-102 93
EDTA plasma (n=8) 91-104 96
Cell culture media (n=8) 87-98 93


Samples were spiked with high concentrations of Human IL-1α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P01583
SynonymsIL1A, IL1-A, IL1, IL1F1, Preinterleukin 1 Alpha, Hematopoietin-1, Pro-Interleukin-1-Alpha
Research AreaCancer, Metabolism, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. ACS Nano (2017) IF: 13.942
    Overcoming the Heat Endurance of Tumor Cells by Interfering with the Anaerobic Glycolysis Metabolism for Improved Photothermal Therapy

    DOI: 10.1021/acsnano.6b06658

    Sample: Cell culture supernatant
  2. Theranostics (2021) IF: 11.556
    A SCID mouse-human lung xenograft model of SARS-CoV-2 infection

    DOI: 10.7150/thno.58321

    PMID: 33995679

    Sample: Tissue homogenate
    Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

    DOI: 10.1002/jcp.29713

    PMID: 32385929

    Sample: Cell culture supernatant
  4. Frontiers in Pharmacology (2020) IF: 4.225
    Exploring the Anti-Acne Potential of Impepho [Helichrysum odoratissimum (L.) Sweet] to Combat Cutibacterium acnes Virulence

    DOI: 10.3389/fphar.2019.01559

    Sample: keratinocytes
  5. Drug Design Development and Therapy (2022) IF: 4.319
    Study on the Mechanism of Improving HIV/AIDS Immune Function with Jian Aikang Concentrated Pill Based on Network Pharmacology Combined with Experimental Validation

    DOI: 10.2147/DDDT.S369832

    PMID: 36003311

    Sample: MT-4 cell
    Inflammation-dependent ISG15 upregulation mediates MIA-induced dendrite damages and depression by disrupting NEDD4/Rap2A signaling

    DOI: 10.1016/j.bbadis.2019.02.020

    PMID: 30826466

    Sample: Serum
    Ultrasensitive detection of interleukin 1α using 3-phosphonopropionic acid modified FTO surface as an effective platform for disposable biosensor fabrication

    DOI: 10.1016/j.bioelechem.2020.107698

    PMID: 33254051

    Sample: Serum,Saliva
  8. FEBS LETTERS (2015) IF: 3.169
    The lncRNA MALAT1 protects the endothelium against ox‐LDL‐induced dysfunction via upregulating the expression of the miR‐22‐3p target genes CXCR2 and AKT

    DOI: 10.1016/j.febslet.2015.08.046

    Sample: Cell culture supernatant
    Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro

    DOI: 10.1016/j.biopha.2019.108706

    PMID: 30844656

    Sample: Wound drainage fluid
  10. CORNEA (2020) IF: 2.215
    Galectin-3, IL-1A, IL-6, and EGF Levels in Corneal Epithelium of Patients With Recurrent Corneal Erosion Syndrome

    DOI: 10.1097/ICO.0000000000002422

    PMID: 32732704

    Sample: Tissue homogenate
  11. APMIS (2019) IF: 2.225
    Autoantibodies to a specific peptide epitope of human Hsp60 (ATVLA) with homology to Helicobacter pylori HspB in H. pylori‐infected patients

    DOI: 10.1111/apm.12925

    Sample: Cell culture supernatant
    Particulate matter 2.5 regulates lipid synthesis and inflammatory cytokine production in human SZ95 sebocytes

    DOI: 10.3892/ijmm.2017.3109

    Sample: Cell culture supernatant
    CFNAs of RBCs affect the release of inflammatory factors through the expression of CaMKIV in macrophages

    DOI: 10.1016/j.transci.2022.103494

    Sample: macrophages
  14. BMC INFECTIOUS DISEASES (2020) IF: 2.688
    Testing the regulatory framework in South Africa – a single-blind randomized pilot trial of commercial probiotic supplementation to standard therapy in women with bacterial vaginosis

    DOI: 10.1186/s12879-020-05210-4

    Sample: cervicovaginal
  15. Medical Science Monitor Basic Research (2022)
    Production of Inflammatory Mediators in Conditioned Medium of Adipose Tissue-Derived Mesenchymal Stem Cells (ATMSC)-Treated Fresh Frozen Plasma

    DOI: 10.12659/MSMBR.933726

    Sample: Adipose Tissue-Derived Mesenchymal Stem Cells (ATM
  16. Journal of Clinical and Experimental Hepatology (2022)
    Demonstration of gut-barrier dysfunction in early stages of non-alcoholic fatty liver disease: A proof-of-concept study

    DOI: 10.1016/j.jceh.2022.01.006

    Sample: serum
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