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Cat.No.:E-EL-H0088
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 3.91-250 pg/mL |
Sensitivity | 2.35 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human IL-1α in samples. No significant cross-reactivity or interference between Human IL-1α and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human IL-1α concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-1α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-1α were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 11.90 | 22.70 | 113.00 | 11.60 | 22.40 | 105.70 |
Standard deviation | 0.70 | 1.20 | 3.60 | 0.80 | 1.30 | 3.70 |
CV (%) | 5.88 | 5.29 | 3.19 | 6.90 | 5.80 | 3.50 |
The recovery of Human IL-1α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 88-102 | 93 |
EDTA plasma (n=8) | 91-104 | 96 |
Cell culture media (n=8) | 87-98 | 93 |
Samples were spiked with high concentrations of Human IL-1α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P01583
|
Synonyms | IL1A, IL1-A, IL1, IL1F1, Preinterleukin 1 Alpha, Hematopoietin-1, Pro-Interleukin-1-Alpha |
Research Area | Cancer, Metabolism, Immunology |
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1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
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Sample: Cell culture supernatant |
DOI: 10.7150/thno.58321 |
|
PMID: 33995679 |
Sample: Tissue homogenate |
DOI: 10.1002/jcp.29713 |
|
PMID: 32385929 |
Sample: Cell culture supernatant |
Sample: keratinocytes |
DOI: 10.2147/DDDT.S369832 |
|
PMID: 36003311 |
Sample: MT-4 cell |
PMID: 30826466 |
Sample: Serum |
PMID: 33254051 |
Sample: Serum,Saliva |
Sample: Cell culture supernatant |
PMID: 30844656 |
Sample: Wound drainage fluid |
PMID: 32732704 |
Sample: Tissue homogenate |
DOI: 10.1111/apm.12925 |
|
Sample: Cell culture supernatant |
Sample: Cell culture supernatant |
Sample: macrophages |
Sample: cervicovaginal |
Sample: Adipose Tissue-Derived Mesenchymal Stem Cells (ATM |
Sample: serum |