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Human IL-1α(Interleukin 1 Alpha) ELISA Kit

Citations(16)Uniprot : P01583

Cat.No.:E-EL-H0088
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	3.91-250 pg/mL
Sensitivity	2.35 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human IL-1α in samples. No significant cross-reactivity or interference between Human IL-1α and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human IL-1α concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-1α. You can calculate the concentration of Human IL-1α in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-1α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-1α were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	11.90	22.70	113.00	11.60	22.40	105.70
Standard deviation	0.70	1.20	3.60	0.80	1.30	3.70
CV (%)	5.88	5.29	3.19	6.90	5.80	3.50

Recovery

The recovery of Human IL-1α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-102	93
EDTA plasma (n=8)	91-104	96
Cell culture media (n=8)	87-98	93

Linearity

Samples were spiked with high concentrations of Human IL-1α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P01583
Synonyms	IL1A, IL1-A, IL1, IL1F1, Preinterleukin 1 Alpha, Hematopoietin-1, Pro-Interleukin-1-Alpha
Research Area	Cancer, Metabolism, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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ACS Nano (2017) IF: 13.942

Overcoming the Heat Endurance of Tumor Cells by Interfering with the Anaerobic Glycolysis Metabolism for Improved Photothermal Therapy

DOI: 10.1021/acsnano.6b06658
	Sample: Cell culture supernatant

Theranostics (2021) IF: 11.556

A SCID mouse-human lung xenograft model of SARS-CoV-2 infection

DOI: 10.7150/thno.58321
PMID: 33995679	Sample: Tissue homogenate

JOURNAL OF CELLULAR PHYSIOLOGY (2020) IF: 5.546

Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

DOI: 10.1002/jcp.29713
PMID: 32385929	Sample: Cell culture supernatant

Frontiers in Pharmacology (2020) IF: 4.225

Exploring the Anti-Acne Potential of Impepho [Helichrysum odoratissimum (L.) Sweet] to Combat Cutibacterium acnes Virulence

DOI: 10.3389/fphar.2019.01559
	Sample: keratinocytes

Drug Design Development and Therapy (2022) IF: 4.319

Study on the Mechanism of Improving HIV/AIDS Immune Function with Jian Aikang Concentrated Pill Based on Network Pharmacology Combined with Experimental Validation

DOI: 10.2147/DDDT.S369832
PMID: 36003311	Sample: MT-4 cell

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2019) IF: 4.328

Inflammation-dependent ISG15 upregulation mediates MIA-induced dendrite damages and depression by disrupting NEDD4/Rap2A signaling

DOI: 10.1016/j.bbadis.2019.02.020
PMID: 30826466	Sample: Serum

BIOELECTROCHEMISTRY (2020) IF: 4.722

Ultrasensitive detection of interleukin 1α using 3-phosphonopropionic acid modified FTO surface as an effective platform for disposable biosensor fabrication

DOI: 10.1016/j.bioelechem.2020.107698
PMID: 33254051	Sample: Serum,Saliva

FEBS LETTERS (2015) IF: 3.169

The lncRNA MALAT1 protects the endothelium against ox‐LDL‐induced dysfunction via upregulating the expression of the miR‐22‐3p target genes CXCR2 and AKT

DOI: 10.1016/j.febslet.2015.08.046
	Sample: Cell culture supernatant

BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 3.743

Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro

DOI: 10.1016/j.biopha.2019.108706
PMID: 30844656	Sample: Wound drainage fluid

CORNEA (2020) IF: 2.215

Galectin-3, IL-1A, IL-6, and EGF Levels in Corneal Epithelium of Patients With Recurrent Corneal Erosion Syndrome

DOI: 10.1097/ICO.0000000000002422
PMID: 32732704	Sample: Tissue homogenate

APMIS (2019) IF: 2.225

Autoantibodies to a specific peptide epitope of human Hsp60 (ATVLA) with homology to Helicobacter pylori HspB in H. pylori‐infected patients

DOI: 10.1111/apm.12925
	Sample: Cell culture supernatant

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2017) IF: 2.341

Particulate matter 2.5 regulates lipid synthesis and inflammatory cytokine production in human SZ95 sebocytes

DOI: 10.3892/ijmm.2017.3109
	Sample: Cell culture supernatant

TRANSFUSION AND APHERESIS SCIENCE (2022) IF: 2.596

CFNAs of RBCs affect the release of inflammatory factors through the expression of CaMKIV in macrophages

DOI: 10.1016/j.transci.2022.103494
	Sample: macrophages

BMC INFECTIOUS DISEASES (2020) IF: 2.688

Testing the regulatory framework in South Africa – a single-blind randomized pilot trial of commercial probiotic supplementation to standard therapy in women with bacterial vaginosis

DOI: 10.1186/s12879-020-05210-4
	Sample: cervicovaginal

Medical Science Monitor Basic Research (2022)

Production of Inflammatory Mediators in Conditioned Medium of Adipose Tissue-Derived Mesenchymal Stem Cells (ATMSC)-Treated Fresh Frozen Plasma

DOI: 10.12659/MSMBR.933726
	Sample: Adipose Tissue-Derived Mesenchymal Stem Cells (ATM

Journal of Clinical and Experimental Hepatology (2022)

Demonstration of gut-barrier dysfunction in early stages of non-alcoholic fatty liver disease: A proof-of-concept study

DOI: 10.1016/j.jceh.2022.01.006
	Sample: serum

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