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Human IL-1α(Interleukin 1 Alpha) ELISA Kit

  • Cat.No.:E-EL-H0088

  • Reactivity: Human

To Purchase E-EL-H0088

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 3.91-250 pg/mL
Sensitivity 2.35 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-1α in samples. No significant cross-reactivity or interference between Human IL-1α and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-1α concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-1α. You can calculate the concentration of Human IL-1α in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-1α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-1α were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 11.90 22.70 113.00 11.60 22.40 105.70
Standard deviation 0.70 1.20 3.60 0.80 1.30 3.70
CV (%) 5.88 5.29 3.19 6.90 5.80 3.50

Recovery

The recovery of Human IL-1α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-102 93
EDTA plasma (n=8) 91-104 96
Cell culture media (n=8) 87-98 93

Linearity

Samples were spiked with high concentrations of Human IL-1α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P01583
SynonymsIL1A, IL1-A, IL1, IL1F1, Preinterleukin 1 Alpha, Hematopoietin-1, Pro-Interleukin-1-Alpha
Research AreaCancer,Immunology,Cell biology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Acs Nano (2017) IF: 13.942
    Overcoming The Heat Endurance Of Tumor Cells By Interfering The Anaerobic Glycolysis Metabolism For Improved Photothermal Therapy.

    DOI: 10.1021/acsnano.6b06658

    PMID: 28107631

    Sample: Cell culture supernatant
  2. Journal of Cellular Physiology (2020) IF: 5.546
    Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

    DOI: 10.1002/jcp.29713

    PMID: 32385929

    Sample: Cell culture supernatant
  3. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2019) IF: 4.328
    Inflammation-dependent ISG15 upregulation mediates MIA-induced dendrite damages and depression by disrupting NEDD4/Rap2A signaling

    DOI: 10.1016/j.bbadis.2019.02.020

    PMID: 30826466

    Sample: Serum
  4. Biomedicine & Pharmacotherapy (2019) IF: 3.457
    Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro

    DOI: 10.1016/j.biopha.2019.108706

    PMID: 30844656

    Sample: Wound drainage fluid
  5. Febs Letters (2015) IF: 3.623
    The lncRNA MALAT1 Protects The Endothelium Against ox-LDL-Induced Dysfunction Via Upregulating The Expression Of The miR-22-3p Target Genes CXCR2 And AKT.

    DOI: 10.1016/j.febslet.2015.08.046

    PMID: 26364720

    Sample: Cell culture supernatant
  6. Cornea (2020) IF: 2.215
    Galectin-3, IL-1A, IL-6, and EGF Levels in Corneal Epithelium of Patients With Recurrent Corneal Erosion Syndrome

    DOI: 10.1097/ICO.0000000000002422

    PMID: 32732704

    Sample: Tissue homogenate
  7. Apmis (2019) IF: 2.026
    Autoantibodies to a Specific Peptide Epitope of Human Hsp60 (ATVLA) With Homology to Helicobacter Pylori HspB in H. Pylori-Infected Patients

    DOI: 10.1111/apm.12925

    PMID: 30746790

    Sample: Cell culture supernatant
  8. International Journal of Molecular Medicine (2017) IF: 2.341
    Particulate Matter 2.5 Regulates Lipid Synthesis And Inflammatory Cytokine Production In Human SZ95 Sebocytes.

    DOI: 10.3892/ijmm.2017.3109

    PMID: 28849137

    Sample: Cell culture supernatant
  9. Modern Research in Inflammation (2019)
    Estimation of Cytokines Involved in Acute-Phase Wound Infection with Reference to Residence Time of Patients in Hospitals

    DOI: 10.4236/mri.2019.81001

    PMID: 99999999

    Sample: Serum
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