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Human IL-23(Interleukin 23) ELISA Kit

  • Cat.No.:E-EL-H0107

  • Reactivity: Human

To Purchase E-EL-H0107

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 39.06-2500 pg/mL
Sensitivity 23.44 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-23 in samples. No significant cross-reactivity or interference between Human IL-23 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-23 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-23. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-23. You can calculate the concentration of Human IL-23 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-23 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-23 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 136.57 343.26 1072.26 129.52 356.17 1144.30
Standard deviation 7.20 15.55 35.49 8.48 20.02 54.47
CV (%) 5.27 4.53 3.31 6.55 5.62 4.76


The recovery of Human IL-23 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-100 94
EDTA plasma (n=8) 94-105 99
Cell culture media (n=8) 87-100 93


Samples were spiked with high concentrations of Human IL-23 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q9NPF7  P29460
SynonymsIL-23A, IL23P19, P19, SGRF
Research AreaImmunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


  1. Annals of Medicine and Surgery (2022)
    The roles of Single Nucleotide Polymorphism (SNP) Endoplasmic Reticulum Aminopeptidase 1 (ERAP 1) gene in axial spondyloarthritis Indonesian adults

    DOI: 10.1016/j.amsu.2022.103675

    PMID: 35638067

    Sample: Peripheral Blood Mononuclear Cell (Pbmc)
    New Findings on Autoimmune Etiology of Idiopathic Granulomatous Mastitis: Serum IL-17, IL-22 and IL-23 Levels of Patients

    DOI: 10.1080/08941939.2020.1725190

    Sample: Serum
    A case of asthma with Behcet’s disease: successful treatment with omalizumab and its effects on recurrent aphthous lesions

    DOI: 10.1080/08923973.2020.1789656

    Sample: Serum
    Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Alleviate Psoriasis-like Skin Inflammation

    DOI: 10.1089/jir.2021.0146

    PMID: 35041513

    Sample: Hacat Cell
  5. ANDROLOGIA (2022) IF: 2.775
    Relationship of interleukin-23 with matrix metalloproteinase-9, pentraxin-3, sleep quality and prostate size in benign prostatic hyperplasia

    DOI: 10.1111/and.14387

    PMID: 35092070

    Sample: Serum
  6. Pharmacological Reports (2021) IF: 3.027
    The association of the quality of sleep with proinflammatory cytokine profile in inflammatory bowel disease patients

    DOI: 10.1007/s43440-021-00333-0

    PMID: 34694623

    Sample: Serum
  7. PLoS Neglected Tropical Diseases (2021) IF: 4.411
    Anaphylatoxins orchestrate Th17 response via interactions between CD16+ monocytes and pleural mesothelial cells in tuberculous pleural effusion

    DOI: 10.1371/journal.pntd.0009508

    PMID: 34237073

    Sample: Pleural Fluid
    Arsenic trioxide improves Treg and Th17 balance by modulating STAT3 in treatment-naïve rheumatoid arthritis patients

    DOI: 10.1016/j.intimp.2019.05.001

    Sample: Pbmcs Cells
  9. JOURNAL OF IMMUNOLOGY (2022) IF: 5.422
    Fungal Patterns Induce Cytokine Expression through Fluxes of Metabolic Intermediates That Support Glycolysis and Oxidative Phosphorylation

    DOI: 10.4049/jimmunol.2100666

    PMID: 35688467

    Sample: Human Monocyte-Derived Dendritic Cells (Dcs)
    Cyclin-Dependent Kinase 7 Promotes Th17/Th1 Cell Differentiation in Psoriasis by Modulating Glycolytic Metabolism

    DOI: 10.1016/j.jid.2021.04.018

    PMID: 34004188

    Sample: Serum
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