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Human IL-2(Interleukin 2) ELISA Kit

  • Cat.No.:E-EL-H0099

  • Reactivity: Human

To Purchase E-EL-H0099

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 7.81-500 pg/mL
Sensitivity 4.69 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-2 in samples. No significant cross-reactivity or interference between Human IL-2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-2. You can calculate the concentration of Human IL-2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 23.64 58.38 217.66 23.12 56.78 226.32
Standard deviation 1.29 2.76 11.30 1.26 2.61 9.30
CV (%) 5.46 4.73 5.19 5.45 4.60 4.11

Recovery

The recovery of Human IL-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-101 93
EDTA plasma (n=8) 93-104 100
Cell culture media (n=8) 93-102 99

Linearity

Samples were spiked with high concentrations of Human IL-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P60568
SynonymsIL2, TCGF, Lymphokine
Research AreaCancer,Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of Cellular Physiology (2020) IF: 5.546
    Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

    DOI: 10.1002/jcp.29713

    PMID: 32385929

    Sample: Cell culture supernatant
  2. The Journal of Biological Chemistry (2016) IF: 4.125
    Down-Regulation Of MicroRNA-451a Facilitates The Activation And Proliferation Of CD4+ T Cells By Targeting Myc In Patients With Dilated Cardiomyopathy.

    DOI: 10.1074/jbc.M116.765107

    PMID: 27974462

    Sample: Cell culture supernatant
  3. Plos Neglected Tropical Diseases (2016) IF: 3.834
    Clonorchis Sinensis Co-Infection Could Affect The Disease State And Treatment Response Of Hbv Patients.

    DOI: 10.1371/journal.pntd.0004806

    PMID: 27348302

    Sample: Serum,Cell culture supernatant
  4. Journal of Zhejiang University. Science. B (2020) IF: 2.082
    Clinical characteristics of 34 COVID-19 patients admitted to intensive care unit in Hangzhou, China

    DOI: 10.1631/jzus.B2000174

    PMID: 32425003

    Sample: Serum,Plasma
  5. Poultry Science (2020) IF: 2.659
    The effect of different dietary ratios of arginine, methionine, and lysine on the performance, carcass traits, and immune status of turkeys

    DOI: 10.1016/j.psj.2019.10.008

    PMID: 32036960

    Sample: Plasma
  6. Apmis (2019) IF: 2.026
    Autoantibodies to a Specific Peptide Epitope of Human Hsp60 (ATVLA) With Homology to Helicobacter Pylori HspB in H. Pylori-Infected Patients

    DOI: 10.1111/apm.12925

    PMID: 30746790

    Sample: Cell culture supernatant
  7. Journal of International Medical Research (2020) IF: 1.287
    Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells

    DOI: 10.1177/0300060520930853

    PMID: 32663414

    Sample: Cell culture supernatant
  8. Medicine (2017) IF: 1.804
    Ketamine, As Adjuvant Analgesics For Patients With Refractory Cancer Pain, Does Affect IL-2/IFN-γ Expression Of T Cells In Vitro? A Prospective, Randomized, Double-Blind Study.

    DOI: 10.1097/MD.9999999900006639

    PMID: 28422864

    Sample: Cell culture supernatant
  9. Meta Gene (2020)
    Interleukin-2 serum level, genetic polymorphism (rs2069763), anti-rubella antibody and risk of multiple sclerosis among Iraqi patients

    DOI: 10.1016/j.mgene.2019.100642

    PMID: 99999999

    Sample: Serum
  10. International Journal of Drug Delivery Technology (2020)
    Evaluation of Synergistic Effect of Cytosine-PhosphodiesterbondGuanosin-Oligodeoxynucleotide 7909, and Protamine on Transfection Process Mediated by Calcium Phosphate Nanoparticles

    PMID: 99999999

    Sample: Cell culture supernatant
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