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Human IL-37(Interleukin 37) ELISA Kit

  • Cat.No.:E-EL-H2571

  • Reactivity: Human

To Purchase E-EL-H2571

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-37 in samples. No significant cross-reactivity or interference between Human IL-37 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-37 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-37. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-37 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-37, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-37. You can calculate the concentration of Human IL-37 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-37 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-37 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 52.90 83.90 344.58 56.16 87.97 362.59
Standard deviation 3.18 4.04 10.75 3.32 3.66 11.13
CV (%) 6.01 4.82 3.12 5.91 4.16 3.07


The recovery of Human IL-37 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-106 99
EDTA plasma (n=8) 96-108 102
Cell culture media (n=8) 85-98 92


Samples were spiked with high concentrations of Human IL-37 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q9NZH6
SynonymsIL37 , FIL1, FIL1(ZETA) , FIL1Z, IL-1F7, IL-1H, IL-1H4, IL-1RP1, IL-37, IL1F7, IL1H4, IL1RP1
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

    A supramolecular hydrogel based on the combination of YIGSR and RGD enhances mesenchymal stem cells paracrine function via integrin α2β1 and PI3K/AKT signaling pathway for acute kidney injury therapy

    DOI: 10.1016/j.cej.2022.135088

    Sample: mesenchymal stem cells (MSCs)
  2. Frontiers in Immunology (2021) IF: 7.561
    Circulating Interleukin-37 Levels in Healthy Adult Humans – Establishing a Reference Range

    DOI: 10.3389/fimmu.2021.708425

    Sample: serum
  3. Journal of Clinical Medicine (2021) IF: 4.242
    Different Features of Interleukin-37 and Interleukin-18 as Disease Activity Markers of Adult-Onset Still’s Disease

    DOI: 10.3390/jcm10050910

    Sample: Serum
  4. Clinical Interventions in Aging (2020) IF: 3.023
    Plasma Interleukin-37 is Elevated in Acute Ischemic Stroke Patients and Probably Associated With 3-month Functional Prognosis

    DOI: 10.2147/CIA.S230186

    PMID: 32801675

    Sample: Plasma
  5. CYTOKINE (2018) IF: 3.514
    Elevated levels of IL-37 correlate with T cell activation status in rheumatoid arthritis patients

    DOI: 10.1016/j.cyto.2018.07.027

    PMID: 30077546

    Sample: Serum
  6. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Crosstalk between vitamin D axis, inflammation and host immunity mechanisms: A prospective study

    DOI: 10.3892/etm.2021.10040

    PMID: 33936265

    Sample: Serum
    The role of serum interleukin-37 levels, inflammation and blood pressure in patients with preeclampsia

    DOI: 10.1080/10641963.2020.1772813

    Sample: blood
  8. Egyptian Journal of Medical Human Genetics (2022)
    Increased expression of IL-37 correlates with TNF-α levels and disease stage in endometriosis patients

    DOI: 10.1186/s43042-022-00285-x

    Sample: serum
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