Human IL-8(Interleukin 8) ELISA Kit

    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
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    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-H6008

      Synonyms:IL8, CXCL8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, 3-10C, AMCF-I, K60

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $495

      Reactivity: Human

      Detection Range: 7.81~500 pg/mL

      Sensitivity: 4.69 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Human IL-8 concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-8. You can calculate the concentration of Human IL-8 in the samples by comparing the OD of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Human
      Detection Method Colormetric
      Detection Range 7.81—500 pg/mL
      Sensitivity 4.69 pg/mL
      Sample Volume Required Per Well 100μL
      Sample Type Serum, plasma and other biological fluids

      Specificity

      This kit recognizes Human IL-8 in samples. No significant cross-reactivity or interference between Human IL-8 and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration
      (pg/mL)
      O.D Average Corrected
      500 2.429
      2.571
      2.5 2.439
      250 1.601
      1.637
      1.619 1.558
      125 0.911
      0.965
      0.9379999999999999 0.877
      62.5 0.46
      0.488
      0.474 0.413
      31.25 0.272
      0.273
      0.273 0.212
      15.63 0.173
      0.188
      0.181 0.12
      7.81 0.119
      0.124
      0.122 0.061
      0 0.063
      0.058
      0.061 --

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-8 were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-8 were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (pg/mL) 20.34 60.67 245.62 24.62 56.15 233.52
      Standard deviation 1.21 2.80 12.26 1.39 2.80 9.69
      C V (%) 5.95 4.62 4.99 5.65 4.99 4.15

      Recovery

      The recovery of Human IL-8 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 87-99 94
      EDTA plasma (n=5) 95-102 98
      Cell culture media (n=5) 98-106 102

      Linearity

      Samples were spiked with high concentrations of Human IL-8 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 97-110 93-105 102-113
      Average (%) 102 101 105
      1:4 Range (%) 93-107 92-101 99-111
      Average (%) 99 95 105
      1:8 Range (%) 87-97 90-103 93-103
      Average (%) 92 95 97
      1:16 Range (%) 93-104 94-110 91-102
      Average (%) 97 102 94

      Kit components & Storage

      An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20°C, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20°C(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 2-8°C(shading light)
      Stop Solution 1 vial, 10 mL 2-8°C
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37°C
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

      elisa assay procedure 2

      2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

      elisa assay procedure 3

      3. Aspirate and wash the plate for 3 times

      elisa assay procedure 4

      4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

      elisa assay procedure 5

      5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

      elisa assay procedure 6

      6. Add 50μL Stop Solution

      elisa assay procedure 7

      7. Read the plate at 450nm immediately. Calculation of the results

      Citations

      1. Antidiabetic (Type 2) Effects Of Lactobacillus G15 And Q14 In Rats Through Regulation Of Intestinal Permeability And Microbiota.
        IF: 3.247
        Journal: Food & Function(2016)
        DOI: 10.1039/c6fo00831c
        PMID: 27713957
        Products: E-EL-H0102 E-EL-H6008 E-EL-H0149 
        Sample type: Plasma,Serum
        Reactivity: Human
      2. The lncRNA MALAT1 Protects The Endothelium Against ox-LDL-Induced Dysfunction Via Upregulating The Expression Of The miR-22-3p Target Genes CXCR2 And AKT.
        IF: 3.623
        Journal: Febs Letters(2015)
        DOI: 10.1016/j.febslet.2015.08.046
        PMID: 26364720
        Products: E-EL-H0088 E-EL-H6008 
        Sample type: Cell culture supernatant
        Reactivity: Human
      3. Zinc Oxide Nanoparticle-Induced Atherosclerotic Alterations In Vitro And In Vivo.
        IF: 4.3
        Journal: International Journal of Nanomedicine(2017)
        DOI: 10.2147/IJN.S134897
        PMID: 28652743
        Products: E-EL-H6008 E-EL-H0109 E-EL-H2172 E-EL-H1640 E-EL-R0580 E-EL-R0504 E-EL-R0488 E-EL-R0902 
        Sample type: Cell culture supernatant,Serum
        Reactivity: Human,Rat
      4. MicroRNA-218 Acts By Repressing TNFR1-Mediated Activation Of NF-κB, Which Is Involved In MUC5AC Hyper-Production And Inflammation In Smoking-Induced Bronchiolitis Of COPD.
        IF: 3.858
        Journal: Toxicology Letters(2017)
        DOI: 10.1016/j.toxlet.2017.08.079
        PMID: 28864214
        Products: E-EL-H2279 E-EL-H0102 E-EL-H6008 
        Sample type: Serum,Cell culture supernatant
        Reactivity: Human
      5. Particulate Matter 2.5 Regulates Lipid Synthesis And Inflammatory Cytokine Production In Human SZ95 Sebocytes.
        IF: 2.341
        Journal: International Journal of Molecular Medicine(2017)
        DOI: 10.3892/ijmm.2017.3109
        PMID: 28849137
        Products: E-EL-H0088 E-EL-H0102 E-EL-H6008 
        Sample type: Cell culture supernatant
        Reactivity: Human
      6. TLR3-/4-Priming Differentially Promotes Ca2+Signaling And Cytokine Expression And Ca2+-Dependently Augments Cytokine Release In hMSCs.
        IF: 4.259
        Journal: Scientific Reports(2016)
        DOI: 10.1038/srep23103
        PMID: 26980664
        Products: E-EL-H0102 E-EL-H6008 E-EL-H6006 
        Sample type: Cell culture supernatant
        Reactivity: Human
      7. High-Mobility Group Protein Box 1 is Upregulated in Children with Henoch-Schonlein Purpura.
        IF: 0.533
        Journal: Pediatric Allergy(2018)
        DOI: 10.1089/ped.2018.0872
        Products: E-EL-H1554 E-EL-H0102 E-EL-H6008 E-EL-H0109 
        Sample type: Serum
        Reactivity: Human
      8. Associations between knee structural measures, circulating inflammatory factors and MMP13 in patients with knee osteoarthritis.
        IF: 5.454
        Journal: Osteoarthritis and cartilage(2018)
        DOI: 10.1016/j.joca.2018.05.003
        PMID: 29753949
        Products: E-EL-H0109 E-EL-H0102 E-EL-H6008 E-EL-H5812 E-EL-H0253 
        Sample type: Serum
        Reactivity: Human
      9. Activation of NF-κB pathways mediating the inflammation and pulmonary diseases associated with atmospheric methylamine exposure
        IF: 5.714
        Journal: Environmental Pollution(2019)
        DOI: 10.1016/j.envpol.2019.06.059
        PMID: 31252119
        Products: E-EL-H0102 E-EL-H6008 
        Sample type: Cell culture supernatant
        Reactivity: Human
      10. Activation of NF-κB pathways mediating the inflammation and pulmonary diseases associated with atmospheric methylamine exposure
        IF: 5.714
        Products: E-EL-H0102 E-EL-H6008 
        Sample type: Cell supernatant
        Reactivity: Human
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