Current Position:Home>>Kits>>ELISA Kits>> Human KL(Klotho) ELISA Kit

Human KL(Klotho) ELISA Kit

Citations(19)Uniprot : Q9UEF7

Cat.No.:E-EL-H5451
Reactivity: Human

Filter:

ELISA

To Purchase E-EL-H5451

Size:

96T
48T
24T
96T*5
96T*10

Price: $609

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.31-20 ng/mL
Sensitivity	0.19 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human KL in samples. No significant cross-reactivity or interference between Human KL and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human KL concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human KL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human KL. You can calculate the concentration of Human KL in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human KL were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human KL were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.04	2.05	9.74	0.97	2.04	9.08
Standard deviation	0.06	0.11	0.34	0.05	0.10	0.48
CV (%)	5.77	5.37	3.49	5.15	4.90	5.29

Recovery

The recovery of Human KL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-99	91
EDTA plasma (n=8)	89-102	96
Cell culture media (n=8)	85-98	90

Linearity

Samples were spiked with high concentrations of Human KL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q9UEF7
Research Area	Cancer, Metabolism, Developmental biology, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

ANNALS OF THE RHEUMATIC DISEASES (2022) IF: 19.103
AB0240 CORRELATION BETWEEN KLOTHO LEVELS AND ANKLE-BRACHIAL INDEX IN FEMALE RHEUMATOID ARTHRITIS PATIENTS

DOI: 10.1136/annrheumdis-2022-eular.446

Sample: serum
Frontiers in Psychiatry (2022) IF: 5.435
Efficacy of a novel proprietary dietary supplement (TRI 360TM) on psychological symptoms and stress-related quality of life in adult subjects: A randomized controlled clinical trial

DOI: 10.3389/fpsyt.2022.919284

PMID: 36032243
Sample: serum
Frontiers in Physiology (2022) IF: 4.566
CCL5 Suppresses Klotho Expression via p-STAT3/DNA Methyltransferase1-Mediated Promoter Hypermethylation

DOI: 10.3389/fphys.2022.856088

PMID: 35299661
Sample: Serum
Frontiers in Neuroscience (2021) IF: 4.677
Kernelized k-Local Hyperplane Distance Nearest-Neighbor Model for Predicting Cerebrovascular Disease in Patients With End-Stage Renal Disease

DOI: 10.3389/fnins.2021.773208

PMID: 34759797
Sample: serum
CLINICAL AND EXPERIMENTAL RHEUMATOLOGY (2023) IF: 4.862
Alpha-Klotho protein in systemic lupus erythematosus

DOI: 10.55563/clinexprheumatol/salqon

Sample: Serum
KIDNEY & BLOOD PRESSURE RESEARCH (2018) IF: 3
The Association of Fibroblast Growth Factor 23 with Arterial Stiffness and Atherosclerosis in Patients with Autosomal Dominant Polycystic Kidney Disease

DOI: 10.1159/000492244

Sample: serum
MEDICAL ONCOLOGY (2021) IF: 3.064
Enhancement of Apo2L/TRAIL signaling pathway receptors by the activation of Klotho gene with CRISPR/Cas9 in Caco-2 colon cancer cells

DOI: 10.1007/s12032-021-01595-7

Sample: Caco-2 cell
Journal of Medical Biochemistry (2020) IF: 3.402
Serum Klotho as a marker for early diagnosis of acute kidney injury after cardiac surgery

DOI: 10.2478/jomb-2019-0024

Sample: Serum
ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS (2023) IF: 3.831
A Comprehensive Study on the Anti-cancer Effects of Quercetin and Its Epigenetic Modifications in Arresting Progression of Colon Cancer Cell Proliferation

DOI: 10.1007/s00005-023-00669-w

Sample: L132 cell,HCT 116 cell,COLO 320 cell,COLO 205 cell
JOURNAL OF NEPHROLOGY (2021) IF: 3.902
Body composition, adipokines, FGF23-Klotho and bone in kidney transplantation: Is there a link?

DOI: 10.1007/s40620-021-00972-9

PMID: 33560479
Sample: Serum
INTERNATIONAL OPHTHALMOLOGY (2021) IF: 2.031
Evaluation of serum and aqueous humor klotho levels in pseudoexfoliation syndrome, pseudoexfoliation and primary open-angle glaucoma

DOI: 10.1007/s10792-021-01790-5

PMID: 33738657
Sample: serum
NORDIC JOURNAL OF PSYCHIATRY (2019) IF: 2.061
Klotho, vitamin D and homocysteine levels during acute episode and remission periods in schizophrenia patients

DOI: 10.1080/08039488.2019.1582697

PMID: 30896269
Sample: Serum
Computational and Mathematical Methods in Medicine (2021) IF: 2.238
A Self-Representation-Based Fuzzy SVM Model for Predicting Vascular Calcification of Hemodialysis Patients

DOI: 10.1155/2021/2464821

PMID: 34367315
Sample: serum
BMC Nephrology (2022) IF: 2.388
Association of plasma neutrophil gelatinase-associated lipocalin and thoracic aorta calcification in maintenance hemodialysis patients with and without diabetes

DOI: 10.1186/s12882-022-02773-z

PMID: 35459121
Sample: plasma
JOURNAL OF BONE AND MINERAL METABOLISM (2021) IF: 2.626
Association of plasma neutrophil gelatinase-associated lipocalin with parameters of CKD–MBD in maintenance hemodialysis patients

DOI: 10.1007/s00774-021-01248-9

Sample: plasma
PeerJ (2021) IF: 2.984
The correlation between fibroblast growth factor-23 and ESRD patients with hearing impairment

DOI: 10.7717/peerj.12295

PMID: 34721981
Sample: serum
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION (2020) IF: 1.475
Association of serum FGF-23, klotho, fetuin-A, osteopontin, osteoprotegerin and hs-CRP levels with coronary artery disease

DOI: 10.1080/00365513.2020.1728786

Sample: serum
Taiwanese Journal of Obstetrics & Gynecology (2021) IF: 1.705
Comparison of serum human Klotho levels and thiol/disulfide homeostasis in women with polycystic ovary syndrome and in healthy women

DOI: 10.1016/j.tjog.2021.03.017

PMID: 33966733
Sample: Serum
Journal of General and Family Medicine (2023)
The role of biofield energy treatment on psychological symptoms, mental health disorders, and stress-related quality of life in adult subjects: A randomized controlled clinical trial

DOI: 10.1002/jgf2.606

Sample: serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

C******************dSubmitted [ Sep 26 2018 ]

Asked: The antibody anti-Klotho is against what nucleotides sequence? I need to check if it could be useful in dogs samples...

adminSubmitted [ Sep 26 2018 ]

Answered: Thanks for your question. The immunogen sequence for the detection antibody is human KL 35~75aa and for the detection antibody is human KL 116~174aa. This kit is specific for human samples, not suitable for dog samples.

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha