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Human LL-37(Antibacterial Protein LL-37) ELISA Kit

  • Cat.No.:E-EL-H2438

  • Reactivity: Human

To Purchase E-EL-H2438

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 1.56-100 ng/mL
Sensitivity 0.94 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human LL-37 in samples. No significant cross-reactivity or interference between Human LL-37 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human LL-37 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LL-37. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LL-37 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LL-37, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LL-37. You can calculate the concentration of Human LL-37 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human LL-37 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human LL-37 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 4.72 12.01 44.23 4.99 11.72 45.29
Standard deviation 0.25 0.69 2.43 0.31 0.48 1.39
CV (%) 5.30 5.75 5.49 6.21 4.10 3.07


The recovery of Human LL-37 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-108 101
EDTA plasma (n=8) 94-109 101
Cell culture media (n=8) 92-107 98


Samples were spiked with high concentrations of Human LL-37 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P49913
Research AreaImmunology, Microbiology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Nutrients (2021) IF: 5.719
    Rapid and Effective Vitamin D Supplementation May Present Better Clinical Outcomes in COVID-19 (SARS-CoV-2) Patients by Altering Serum INOS1, IL1B, IFNg, Cathelicidin-LL37, and ICAM1

    DOI: 10.3390/nu13114047

    PMID: 34836309

    Sample: serum
  2. Cells (2020) IF: 4.366
    Candidalysin Is a Potent Trigger of Alarmin and Antimicrobial Peptide Release in Epithelial Cells

    DOI: 10.3390/cells9030699

    PMID: 32178483

    Sample: Cell culture supernatant
  3. PLoS One (2021) IF: 3.24
    Serum and cerebrospinal fluid host proteins indicate stroke in children with tuberculous meningitis

    DOI: 10.1371/journal.pone.0250944

    PMID: 33930055

    Sample: Serum,Cerebrospinal Fluid
  4. Italian Journal of Pediatrics (2022) IF: 3.288
    Cord blood antimicrobial peptide LL37 levels in preterm neonates and association with preterm complications

    DOI: 10.1186/s13052-022-01295-6

    PMID: 35804392

    Sample: serum
  5. Pathogens (2021) IF: 3.492
    Prediction of Treatment Outcome with Inflammatory Biomarkers after 2 Months of Therapy in Pulmonary Tuberculosis Patients: Preliminary Results

    DOI: 10.3390/pathogens10070789

    PMID: 34206598

    Sample: serum
    Application of Cerebrospinal Fluid Host Protein Biosignatures in the Diagnosis of Tuberculous Meningitis in Children from a High Burden Setting

    DOI: 10.1155/2019/7582948

    Sample: cerebrospinal fluid (CSF)
  7. International Journal of Paediatric Dentistry (2019) IF: 2.057
    Salivary LL‐37 and periodontal health in children exposed to passive smoking

    DOI: 10.1111/ipd.12459

    Sample: Saliva
  8. Frontiers in Pediatrics (2019) IF: 2.349
    Potential of Host Serum Protein Biomarkers in the Diagnosis of Tuberculous Meningitis in Children

    DOI: 10.3389/fped.2019.00376

    PMID: 31612118

    Sample: Serum
  9. International Journal of Rheumatic Diseases (2020) IF: 1.98
    Salivary and serum cathelicidin LL‐37 levels in subjects with rheumatoid arthritis and chronic periodontitis

    DOI: 10.1111/1756-185X.13919

    Sample: Serum,Saliva
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  • Q&A

M*************aSubmitted [ Oct 22 2022 ]

Asked: Is this kit compatible with Luminex?



adminSubmitted [ Oct 22 2022 ]

Answered: This kit is not compatible with Luminex, and Luminex is generally used for multifactor detection, and we do not have a relevant kit.

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