Current Position:Home>>Kits>>ELISA Kits>> Human LL-37(Antibacterial Protein LL-37) ELISA Kit

Human LL-37(Antibacterial Protein LL-37) ELISA Kit

Citations(9)Uniprot : P49913

Cat.No.:E-EL-H2438
Reactivity: Human

Filter:

ELISA

To Purchase E-EL-H2438

Size:

96T
48T
24T
96T*5
96T*10

Price: $609

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	1.56-100 ng/mL
Sensitivity	0.94 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human LL-37 in samples. No significant cross-reactivity or interference between Human LL-37 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human LL-37 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LL-37. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LL-37 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LL-37, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LL-37. You can calculate the concentration of Human LL-37 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human LL-37 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human LL-37 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	4.72	12.01	44.23	4.99	11.72	45.29
Standard deviation	0.25	0.69	2.43	0.31	0.48	1.39
CV (%)	5.30	5.75	5.49	6.21	4.10	3.07

Recovery

The recovery of Human LL-37 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	95-108	101
EDTA plasma (n=8)	94-109	101
Cell culture media (n=8)	92-107	98

Linearity

Samples were spiked with high concentrations of Human LL-37 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P49913
Research Area	Immunology, Microbiology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Nutrients (2021) IF: 5.719
Rapid and Effective Vitamin D Supplementation May Present Better Clinical Outcomes in COVID-19 (SARS-CoV-2) Patients by Altering Serum INOS1, IL1B, IFNg, Cathelicidin-LL37, and ICAM1

DOI: 10.3390/nu13114047

PMID: 34836309
Sample: serum
Cells (2020) IF: 4.366
Candidalysin Is a Potent Trigger of Alarmin and Antimicrobial Peptide Release in Epithelial Cells

DOI: 10.3390/cells9030699

PMID: 32178483
Sample: Cell culture supernatant
PLoS One (2021) IF: 3.24
Serum and cerebrospinal fluid host proteins indicate stroke in children with tuberculous meningitis

DOI: 10.1371/journal.pone.0250944

PMID: 33930055
Sample: Serum,Cerebrospinal Fluid
Italian Journal of Pediatrics (2022) IF: 3.288
Cord blood antimicrobial peptide LL37 levels in preterm neonates and association with preterm complications

DOI: 10.1186/s13052-022-01295-6

PMID: 35804392
Sample: serum
Pathogens (2021) IF: 3.492
Prediction of Treatment Outcome with Inflammatory Biomarkers after 2 Months of Therapy in Pulmonary Tuberculosis Patients: Preliminary Results

DOI: 10.3390/pathogens10070789

PMID: 34206598
Sample: serum
MEDIATORS OF INFLAMMATION (2019) IF: 3.545
Application of Cerebrospinal Fluid Host Protein Biosignatures in the Diagnosis of Tuberculous Meningitis in Children from a High Burden Setting

DOI: 10.1155/2019/7582948

Sample: cerebrospinal fluid (CSF)
International Journal of Paediatric Dentistry (2019) IF: 2.057
Salivary LL‐37 and periodontal health in children exposed to passive smoking

DOI: 10.1111/ipd.12459

Sample: Saliva
Frontiers in Pediatrics (2019) IF: 2.349
Potential of Host Serum Protein Biomarkers in the Diagnosis of Tuberculous Meningitis in Children

DOI: 10.3389/fped.2019.00376

PMID: 31612118
Sample: Serum
International Journal of Rheumatic Diseases (2020) IF: 1.98
Salivary and serum cathelicidin LL‐37 levels in subjects with rheumatoid arthritis and chronic periodontitis

DOI: 10.1111/1756-185X.13919

Sample: Serum,Saliva

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

M*************aSubmitted [ Oct 22 2022 ]

Asked: Is this kit compatible with Luminex?

adminSubmitted [ Oct 22 2022 ]

Answered: This kit is not compatible with Luminex, and Luminex is generally used for multifactor detection, and we do not have a relevant kit.

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha