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Human MBP(Myelin Basic Protein) ELISA Kit

  • Cat.No.:E-EL-H0161

  • Reactivity: Human

To Purchase E-EL-H0161

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human MBP in samples. No significant cross-reactivity or interference between Human MBP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human MBP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MBP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MBP. You can calculate the concentration of Human MBP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MBP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MBP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 53.45 148.17 412.60 55.17 140.51 436.13
Standard deviation 2.89 8.48 17.29 3.40 7.52 17.01
CV (%) 5.41 5.72 4.19 6.16 5.35 3.90


The recovery of Human MBP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-101 93
EDTA plasma (n=8) 90-103 95
Cell culture media (n=8) 88-99 94


Samples were spiked with high concentrations of Human MBP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P02686
Research AreaCancer, Neuroscience, Tags and Cell Markers

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. FASEB Journal (2017) IF: 5.498
    Competitive Apnea And Its Effect On The Human Brain: Focus On The Redox-Regulation Of Blood-Brain Barrier Permeability And Neuronal-Parenchymal Integrity.

    DOI: 10.1096/fj.201701031R

    PMID: 29191963

    Sample: Serum
  2. International Journal of Developmental Neuroscience (2017) IF: 2.046
    Evaluation Of Serum Neuron-Specific Enolase, S100B, Myelin Basic Protein And Glial Fibrilliary Acidic Protein As Brain Specific Proteins In Children With Autism Spectrum Disorder.

    DOI: 10.1016/j.ijdevneu.2017.06.011

    PMID: 28711670

    Sample: Serum


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  • Q&A

T**********eSubmitted [ Jun 09 2018 ]

Asked: If I received the MBP kit, where should I store it, and how long? What does the expiration mean? When I open the kit, Can I use the Standard and reagent one more time?



adminSubmitted [ Jun 09 2018 ]

Answered: Thank you for your message. Generally, an unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, pls store the below items separately according to the following conditions once the kit is received. 1. Micro ELISA Plate(Dismountable), Reference Standard, Concentrated Biotinylated Detection Ab (100×) ,pls store at -20℃; 2. Concentrated HRP Conjugate (100×), pls store at -20℃(shading light); 3. For the below reagents, pls store at 4℃; Reference Standard & Sample Diluent; Biotinylated Detection Ab Diluent; HRP Conjugate Diluent; Concentrated Wash Buffer (25×); Substrate Reagent; Stop Solution; The expiration means the shelf life, which is 6 months. For the Standard and reagent, the diluted reagents are not recommended repetitive use. You'd better prepare before use. Any other questions, pls feel free to send it to us ( All the best.

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