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Cat.No.:E-EL-H0161
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 15.63-1000 pg/mL |
Sensitivity | 9.38 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human MBP in samples. No significant cross-reactivity or interference between Human MBP and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human MBP concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MBP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MBP were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 53.45 | 148.17 | 412.60 | 55.17 | 140.51 | 436.13 |
Standard deviation | 2.89 | 8.48 | 17.29 | 3.40 | 7.52 | 17.01 |
CV (%) | 5.41 | 5.72 | 4.19 | 6.16 | 5.35 | 3.90 |
The recovery of Human MBP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 87-101 | 93 |
EDTA plasma (n=8) | 90-103 | 95 |
Cell culture media (n=8) | 88-99 | 94 |
Samples were spiked with high concentrations of Human MBP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P02686
|
Synonyms | Myelin_MBP |
Research Area | Cancer, Neuroscience, Tags and Cell Markers |
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1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
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T**********eSubmitted [ Jun 09 2018 ]
Asked: If I received the MBP kit, where should I store it, and how long? What does the expiration mean? When I open the kit, Can I use the Standard and reagent one more time?
Reply
adminSubmitted [ Jun 09 2018 ]
Answered: Thank you for your message. Generally, an unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, pls store the below items separately according to the following conditions once the kit is received. 1. Micro ELISA Plate(Dismountable), Reference Standard, Concentrated Biotinylated Detection Ab (100×) ,pls store at -20℃; 2. Concentrated HRP Conjugate (100×), pls store at -20℃(shading light); 3. For the below reagents, pls store at 4℃; Reference Standard & Sample Diluent; Biotinylated Detection Ab Diluent; HRP Conjugate Diluent; Concentrated Wash Buffer (25×); Substrate Reagent; Stop Solution; The expiration means the shelf life, which is 6 months. For the Standard and reagent, the diluted reagents are not recommended repetitive use. You'd better prepare before use. Any other questions, pls feel free to send it to us (techsupport@elabscience.com). All the best.