Human MIP-5 (Macrophage Inflammatory Protein 5) ELISA Kit (E-EL-H6314)

Name: Human MIP-5 (Macrophage Inflammatory Protein 5) ELISA Kit
Brand: Elabscience
SKU: E-EL-H6314
Price: 150.0 USD
Availability: InStock

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Human MIP-5 (Macrophage Inflammatory Protein 5) ELISA Kit - 1

Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human MIP-5 in samples.No significant cross-reactivity or interference between and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T*5
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	2.280	2.302	2.291	2.204
500	1.462	1.480	1.471	1.384
250	0.806	0.836	0.821	0.734
125	0.476	0.478	0.477	0.390
62.5	0.277	0.297	0.287	0.200
31.25	0.174	0.186	0.180	0.093
15.63	0.134	0.140	0.137	0.050
0	0.085	0.089	0.087	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	50.650	158.250	477.250	55.270	172.300	470.430
Standard deviation	2.070	7.520	21.000	4.500	12.230	35.940
CV（%）	4.090	4.750	4.400	8.140	7.100	7.640

Recovery

The recovery of Human MIP-5 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-97	92
EDTA plasma （n=8）	89-104	95
Cell culture media （n=8）	86-96	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human MIP-5 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-112	90-107	87-99
1:2	Average	106	98	94
1:4	Range	100-117	94-108	86-97
1:4	Average	107	100	92
1:8	Range	98-114	95-108	87-100
1:8	Average	105	100	94

For the recovery experiment, the samples were spiked with standard solutions of a specific concentration and then serially diluted to 2-fold, 4-fold, 8-fold, and 16-fold dilutions, respectively. The average recovery rate was subsequently calculated.

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	100.25-109.69
Sample 2（n=16）	96.86-98.08

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MIP-5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIP-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIP-5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 3 nm. The OD value is proportional to the concentration of Human MIP-5. You can calculate the concentration of Human MIP-5 in the samples by comparing the OD of the samples to the standard curve.

Background

Macrophage Inflammatory Protein 5 (MIP-5), also designated as C-C motif chemokine ligand 15 (CCL15), is a member of the CC chemokine family, initially discovered by analyzing the upstream region of the CCL14 gene. Located on human chromosome 17, it is arranged in tandem with CCL14 and belongs to the NC6 beta-chemokines, featuring an extended N-terminal domain (16-20 residues) encoded by a distinct exon upstream of the first two conserved cysteines, shared with CCL6, CCL9, and CCL23. The precursor CCL15 (1-92) undergoes proteolytic processing by cathepsin G, elastase, and MT6-MMP/MMP25 to generate more active truncated forms, such as CCL15 (22-92), (24-92), (27-92), and (29-92). As a chemotactic factor, MIP-5/CCL15 primarily attracts T-cells and monocytes (not neutrophils, eosinophils, or B-cells) via binding to CC chemokine receptor 1 (CCR1), with additional binding to CCR3, and its truncated forms exhibit stronger chemoattractant activity. Clinically, it is closely associated with various diseases: elevated in renal cell carcinoma bone metastasis, pulmonary sarcoidosis bronchoalveolar lavage, severe asthma serum (reduced by omalizumab), and hepatocellular carcinoma (HCC) serum, where it promotes tumor progression via the CCL15-CCR1 axis. It is also linked to colorectal cancer and esophageal squamous cell carcinoma, highlighting its potential as a diagnostic and therapeutic target.