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Human MMP-3(Matrix Metalloproteinase 3) ELISA Kit

  • Cat.No.:E-EL-H1446

  • Reactivity: Human

To Purchase E-EL-H1446

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human MMP-3 in samples. No significant cross-reactivity or interference between Human MMP-3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human MMP-3 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-3. You can calculate the concentration of Human MMP-3 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MMP-3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MMP-3 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 1.30 4.50 0.50 1.40 4.80
Standard deviation 0.03 0.07 0.24 0.03 0.07 0.14
CV (%) 6.00 5.38 5.33 6.00 5.00 2.92

Recovery

The recovery of Human MMP-3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-103 96
EDTA plasma (n=8) 94-106 101
Cell culture media (n=8) 88-103 95

Linearity

Samples were spiked with high concentrations of Human MMP-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P08254
SynonymsMMP3, CHDS6, SL-1, STMY, STMY1, STR1, Stromelysin-1
Research AreaCancer, Cell Biology, Cardiovascular, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Nature Communications (2021) IF: 14.919
    Geometrically encoded SERS nanobarcodes for the logical detection of nasopharyngeal carcinoma-related progression biomarkers

    DOI: 10.1038/s41467-021-23789-3

    PMID: 34078895

    Sample: plasma
  2. Science Signaling (2016) IF: 7.359
    Quantitative phosphoproteomic analysis identifies the critical role of JNK1 in neuroinflammation induced by Japanese encephalitis virus

    DOI: 10.1126/scisignal.aaf5132

    Sample: Human glioma cell line U251
  3. Antioxidants (2022) IF: 7.675
    Anti-Aging Effect and Mechanism of Proanthocyanidins Extracted from Sea buckthorn on Hydrogen Peroxide-Induced Aging Human Skin Fibroblasts

    DOI: 10.3390/antiox11101900

    PMID: 36290623

    Sample: HSFs
  4. Marine Drugs (2021) IF: 5.118
    Heptapeptide Isolated from Isochrysis zhanjiangensis Exhibited Anti-Photoaging Potential via MAPK/AP-1/MMP Pathway and Anti-Apoptosis in UVB-Irradiated HaCaT Cells

    DOI: 10.3390/md19110626

    PMID: 34822497

    Sample: HaCaT cells
  5. CHEMICO-BIOLOGICAL INTERACTIONS (2021) IF: 5.194
    A novel glyceroglycolipid from brown algae Ishige okamurae improve photoaging and counteract inflammation in UVB-induced HaCaT cells

    DOI: 10.1016/j.cbi.2021.109737

    PMID: 34740599

    Sample: HaCaT cells
  6. Journal of Functional Foods (2022) IF: 5.223
    High fiber multigrain supplementation improved disease activity score, circulating inflammatory and oxidative stress biomarkers in rheumatoid arthritis (RA) patients: A randomized human clinical trial

    DOI: 10.1016/j.jff.2022.105392

    Sample: blood
  7. OSTEOARTHRITIS AND CARTILAGE (2018) IF: 5.454
    Associations between serum S100A8/S100A9 and knee symptoms, joint structures and cartilage enzymes in patients with knee osteoarthritis

    DOI: 10.1016/j.joca.2018.08.020

    PMID: 30240939

    Sample: Serum
  8. Frontiers in Pharmacology (2022) IF: 5.988
    Aconiti lateralis radix praeparata total alkaloids exert anti-RA effects by regulating NF-κB and JAK/STAT signaling pathways and promoting apoptosis.

    DOI: 10.3389/fphar.2022.980229

    Sample: MH7A cell
  9. Spine Journal (2022) IF: 4.166
    Extracellular vesicle and soluble fractions of adipose tissue-derived mesenchymal stem cells secretome induce inflammatory cytokines modulation in an in vitro model of discogenic pain

    DOI: 10.1016/j.spinee.2022.01.012

    PMID: 35121152

    Sample: human adipose-derived mesenchymal stem cells (HAdM
  10. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Anti-proliferation and anti-inflammation effects of corilagin in rheumatoid arthritis by downregulating NF-κB and MAPK signaling pathways

    DOI: 10.1016/j.jep.2021.114791

    PMID: 34737112

    Sample: MH7A cell
  11. OSTEOARTHRITIS AND CARTILAGE (2017) IF: 4.742
    Associations between serum ghrelin and knee symptoms, joint structures and cartilage or bone biomarkers in patients with knee osteoarthritis

    DOI: 10.1016/j.joca.2017.05.015

    Sample: Serum
  12. PPAR Research (2019) IF: 4.964
    Arsenic Trioxide in Synergy with Vitamin D Rescues the Defective VDR-PPAR- Functional Module of Autophagy in Rheumatoid Arthritis

    DOI: 10.1155/2019/6403504

    Sample: rheumatoid arthritis (RA) fibroblast-like synovioc
  13. Journal of Functional Foods (2020) IF: 3.701
    Trehalose against UVB-induced skin photoaging by suppressing MMP expression and enhancing procollagen I synthesis in HaCaT cells

    DOI: 10.1016/j.jff.2020.104198

    Sample: HaCaT cell
  14. CLINICAL RHEUMATOLOGY (2019) IF: 2.293
    Associations between serum IL-8 and knee symptoms, joint structures, and cartilage or bone biomarkers in patients with knee osteoarthritis

    DOI: 10.1007/s10067-019-04718-8

    PMID: 31377918

    Sample: Serum
  15. INTERNATIONAL DENTAL JOURNAL (2022) IF: 2.607
    Efficacy of Proanthocyanidins in Nonsurgical Periodontal Therapy

    DOI: 10.1016/j.identj.2022.08.006

    PMID: 36167610

    Sample: saliva samples
  16. RHEUMATOLOGY INTERNATIONAL (2022) IF: 2.631
    Assessment of serum and synovial fluid MMP-3 and MPO as biomarkers for psoriatic arthritis and their relation to disease activity indices

    DOI: 10.1007/s00296-022-05159-4

    PMID: 35708757

    Sample: plasma
  17. Applied Sciences-Basel (2022) IF: 2.838
    Effectiveness of a Cucumber Extract Supplement on Articular Pain in Patients with Knee Osteoarthritis: A Randomized Double-Blind Controlled Clinical Trial

    DOI: 10.3390/app13010485

    Sample: plasma
  18. Iranian Journal of Basic Medical Sciences (2018) IF: 1.514
    Effects of insulin-like growth factor-induced Wharton jelly mesenchymal stem cells toward chondrogenesis in an osteoarthritis model

    DOI: 10.22038/IJBMS.2018.28205.6840

    Sample: Serum
  19. EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES (2017) IF: 1.685
    Expression and Significance of MMPs in Synovial Fluid, Serum and PBMC Culture Supernatant Stimulated by LPS in Osteoarthritis Patients With or Without Diabetes

    DOI: 10.1055/s-0043-122223

    PMID: 29270923

    Sample: Serum,Synovia
  20. International Journal of Rheumatic Diseases (2020) IF: 1.98
    Associations of serum citrate levels with knee structural changes and cartilage enzymes in patients with knee osteoarthritis

    DOI: 10.1111/1756-185X.13787

    Sample: serum
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