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Cat.No.:E-EL-H1450
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 0.16-10 ng/mL |
Sensitivity | 0.10 ng/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human MMP-8 in samples. No significant cross-reactivity or interference between Human MMP-8 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human MMP-8 concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MMP-8 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MMP-8 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 0.50 | 1.20 | 4.40 | 0.50 | 1.20 | 4.10 |
Standard deviation | 0.03 | 0.06 | 0.23 | 0.03 | 0.06 | 0.17 |
CV (%) | 6.00 | 5.00 | 5.23 | 6.00 | 5.00 | 4.15 |
The recovery of Human MMP-8 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 86-96 | 91 |
EDTA plasma (n=8) | 89-102 | 96 |
Cell culture media (n=8) | 95-111 | 101 |
Samples were spiked with high concentrations of Human MMP-8 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P22894
|
Synonyms | MMP8, CLG1, HNC, PMNL-CL, Neutrophil Collagenase |
Research Area | Cancer, Cell Biology, Cardiovascular, Signal transduction |
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1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
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DOI: 10.1111/jcmm.16275 |
|
PMID: 33448094 |
Sample: Plasma |
DOI: 10.3390/jof7100842 |
|
Sample: peripheral blood |
DOI: 10.52586/4933 |
|
Sample: saliva |
PMID: 33567492 |
Sample: Saliva |
Sample: serum |
Sample: Saliva |
DOI: 10.1002/JPER.18-0608 |
|
Sample: Gingival crevicular fluid(GCF) |
PMID: 29270923 |
Sample: Serum,Synovia |
DOI: 10.1002/cre2.624 |
|
PMID: 35769040 |
Sample: Salivary |
M********nSubmitted [ Jun 07 2022 ]
Asked: Hello, Does this kit estimated total MMP-8 or active MMP-8 levels ?
Reply
adminSubmitted [ Jun 07 2022 ]
Answered: This kit detects total MMP-8.
M********nSubmitted [ Feb 11 2022 ]
Asked: Does this required dilution ?
Reply
adminSubmitted [ Feb 11 2022 ]
Answered: Hi Maaz, thanks for your question. Yes, this kit requires dilution, and it's recommended to test normal human serum/plasma samples at about 50-200 fold from our in-house data.