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Human MUC2(Mucin 2) ELISA Kit

Citations(9)Uniprot : Q02817

Cat.No.:E-EL-H0632
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.31-20 ng/mL
Sensitivity	0.19 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human MUC2 in samples. No significant cross-reactivity or interference between Human MUC2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human MUC2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MUC2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MUC2. You can calculate the concentration of Human MUC2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MUC2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MUC2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.97	2.92	7.41	1.00	3.20	7.38
Standard deviation	0.06	0.13	0.23	0.06	0.16	0.30
CV (%)	6.19	4.45	3.10	6.00	5.00	4.07

Recovery

The recovery of Human MUC2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	87-101	93
EDTA plasma (n=8)	93-106	100
Cell culture media (n=8)	92-104	97

Linearity

Samples were spiked with high concentrations of Human MUC2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q02817
Synonyms	MLP, MUC-2, SMUC
Research Area	Cancer, Immunology, Signal transduction, Tags and Cell Markers

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

ACS Applied Materials & Interfaces (2022) IF: 9.2289
Hybrid Lipid/Polymer Nanoparticles to Tackle the Cystic Fibrosis Mucus Barrier in siRNA Delivery to the Lungs: Does PEGylation Make the Difference?

DOI: 10.1021/acsami.1c14975

PMID: 35107987
Sample: sputum
BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
Specific activation of hypoxia-inducible factor-2α by propionate metabolism via a β-oxidation-like pathway stimulates MUC2 production in intestinal goblet cells

DOI: 10.1016/j.biopha.2022.113672

PMID: 36095963
Sample: LS 174 T cell
JOURNAL OF ETHNOPHARMACOLOGY (2023) IF: 5.195
Shaoyao decoction restores the mucus layer in mice with DSS-induced colitis by regulating Notch signaling pathway

DOI: 10.1016/j.jep.2023.116258

Sample: LS174T cell
Frontiers in Cellular and Infection Microbiology (2021) IF: 5.293
Antimicrobial Peptides Human Beta-Defensin-2 and -3 Protect the Gut During Candida albicans Infections Enhancing the Intestinal Barrier Integrity: In Vitro Study

DOI: 10.3389/fcimb.2021.666900

Sample: Caco-2 cells
Nutrients (2022) IF: 5.719
Xenogeneic-Free Human Intestinal Organoids for Assessing Intestinal Nutrient Absorption

DOI: 10.3390/nu14030438

PMID: 35276796
Sample: human intestinal organoids (HIOs)
JOURNAL OF BIOMEDICAL SCIENCE (2020) IF: 5.762
Melatonin restores Muc2 depletion induced by V. vulnificus VvpM via melatonin receptor 2 coupling with Gαq

DOI: 10.1186/s12929-019-0606-x

Sample: HT29-MTX cell
Biomolecules (2020) IF: 4.082
Exogenous NAD+ Stimulates MUC2 Expression in LS 174T Goblet Cells via the PLC-Delta/PTGES/PKC-Delta/ERK/CREB Signaling Pathway

DOI: 10.3390/biom10040580

Sample: Cell culture supernatant
Journal of Asthma and Allergy (2017) IF: 4.258
Asthma with bronchial hypersecretion: expression of mucins and toll-like receptors in sputum and blood

DOI: 10.2147/JAA.S142200

Sample: induced sputum (IS)
Scientific Reports (2020) IF: 3.998
Dairy Propionibacterium freudenreichii ameliorates acute colitis by stimulating MUC2 expression in intestinal goblet cell in a DSS-induced colitis rat model

DOI: 10.1038/s41598-020-62497-8

Sample: LS 174 cell

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