Human NAGase(N-Acetyl Beta-D-Glucosaminidase) ELISA Kit (E-EL-H0898)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NAGase. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NAGase and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NAGase, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NAGase. You can calculate the concentration of Human NAGase in the samples by comparing the OD of the samples to the standard curve.

Podocin (PDCN), encoded by the NPHS2 gene, is a 383-amino-acid membrane protein of the band-7/stomatin family that is expressed almost exclusively in glomerular podocytes. The protein adopts a hairpin-like topology with both termini facing the cytoplasm and inserts into the slit-diaphragm region of podocyte foot processes, where it is enriched in lipid raft microdomains and helps organize the filtration slit apparatus. At the slit diaphragm podocin forms oligomers and associates with nephrin, CD2AP and the actin cytoskeleton, acting as a scaffolding protein that links the membrane receptor complex to cytoskeletal elements and thereby regulates the permselectivity of the glomerular filtration barrier toward plasma proteins. Its lipid-raft localization also places it within a signaling platform that participates in slit-diaphragm signal transduction, and disruption of these interactions leads to foot-process effacement and proteinuria. NPHS2 mutations cause steroid-resistant nephrotic syndrome, which frequently progresses to focal segmental glomerulosclerosis and end-stage kidney disease. Assessment of podocin expression and of NPHS2 variants is therefore important for the diagnosis and classification of nephrotic syndrome and for studies of podocyte injury mechanisms. This ELISA kit enables quantitative determination of podocin in biological samples, and reduced expression is recognized as an early event in podocyte damage.