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Human NGF(Nerve Growth Factor) ELISA Kit

Citations(12)Uniprot : P01138

Cat.No.:E-EL-H1205
Reactivity: Human

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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human NGF in samples. No significant cross-reactivity or interference between Human NGF and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human NGF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NGF. You can calculate the concentration of Human NGF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human NGF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human NGF were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	48.95	103.15	378.79	50.87	111.32	379.86
Standard deviation	2.77	4.32	14.39	2.93	6.37	15.92
CV (%)	5.66	4.19	3.80	5.76	5.72	4.19

Recovery

The recovery of Human NGF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-99	93
EDTA plasma (n=8)	92-104	99
Cell culture media (n=8)	87-103	94

Linearity

Samples were spiked with high concentrations of Human NGF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P01138
Synonyms	Beta-NGF, HSAN5, NGFB, NGF-B
Research Area	Cancer, Neuroscience

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Cell Metabolism (2022) IF: 31.373
Cancer cells co-opt nociceptive nerves to thrive in nutrient-poor environments and upon nutrient-starvation therapies

DOI: 10.1016/j.cmet.2022.10.012

PMID: 36395769
Sample: plasma
Journal of Functional Foods (2022) IF: 5.223
Effects of erinacine A-enriched Hericium erinaceus on elderly hearing-impaired patients: A double-blind, randomized, placebo-controlled clinical trial

DOI: 10.1016/j.jff.2022.105220

Sample: serum
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2018) IF: 5.5
Dual Delivery of bFGF- and NGF-Binding Coacervate Confers Neuroprotection by Promoting Neuronal Proliferation

DOI: 10.1159/000490139

Sample: Serum
American Journal of Translational Research (2021) IF: 4.06
Effect of levetiracetam in combination with topiramate on immune function, cognitive function, and neuronal nutritional status of children with intractable epilepsy

PMID: 34650715
Sample: serum
Scientific Reports (2018) IF: 4.122
Human Wharton’s jelly mesenchymal stem cells protect axotomized rat retinal ganglion cells via secretion of anti-inflammatory and neurotrophic factors

DOI: 10.1038/s41598-018-34527-z

Sample: Tissue homogenate,Cell lysate
Stem Cell Research & Therapy (2017) IF: 4.211
Adult human periodontal ligament-derived stem cells delay retinal degeneration and maintain retinal function in RCS rats

DOI: 10.1186/s13287-017-0731-y

Sample: human periodontal ligament-derived stem cells (hPD
Nutrients (2020) IF: 4.546
The Effects of Tocotrienol-Rich Vitamin E (Tocovid) on Diabetic Neuropathy: A Phase II Randomized Controlled Trial

DOI: 10.3390/nu12051522

PMID: 32456230
Sample: Serum
EUROPEAN NEUROPSYCHOPHARMACOLOGY (2022) IF: 4.6
The role of BDNF and NGF plasma levels in first-episode schizophrenia: A longitudinal study

DOI: 10.1016/j.euroneuro.2022.02.003

PMID: 35219096
Sample: plasma
EXPERIMENTAL NEUROLOGY (2016) IF: 4.657
Single injection of a novel nerve growth factor coacervate improves structural and functional regeneration after sciatic nerve injury in adult rats

DOI: 10.1016/j.expneurol.2016.10.015

Sample: Others
BIOPROCESS AND BIOSYSTEMS ENGINEERING (2023) IF: 3.434
Increased levels of nerve growth factor accompany oxidative load in recurrent pregnancy loss. Machine learning applied to FT-Raman spectra study

DOI: 10.1007/s00449-023-02847-8

Sample: serum
NEUROUROLOGY AND URODYNAMICS (2020) IF: 2.037
The profile of urinary biomarkers in overactive bladder

DOI: 10.1002/nau.24487

PMID: 32813897
Sample: Urine
Sport Sciences for Health (2022)
The effects of aerobic exercise and sertraline on pro-inflammatory indices and amelioration of neurotrophic factors in patients who have undergone CABG

DOI: 10.1007/s11332-022-00992-3

Sample: plasma,serum

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Reviews/Q&A

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Reviews
Q&A

F*******aSubmitted [ May 02 2023 ]

Asked: is there cross reactivity with MOUSE NGF?

adminSubmitted [ May 02 2023 ]

Answered: Dear Francesca, thanks for reaching out! I'll reply to you by email. Pls pay attention to it.

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