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Human NGF(Nerve Growth Factor) ELISA Kit

  • Cat.No.:E-EL-H1205

  • Reactivity: Human

To Purchase E-EL-H1205

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human NGF in samples. No significant cross-reactivity or interference between Human NGF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human NGF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NGF. You can calculate the concentration of Human NGF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human NGF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human NGF were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 48.95 103.15 378.79 50.87 111.32 379.86
Standard deviation 2.77 4.32 14.39 2.93 6.37 15.92
CV (%) 5.66 4.19 3.80 5.76 5.72 4.19


The recovery of Human NGF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-99 93
EDTA plasma (n=8) 92-104 99
Cell culture media (n=8) 87-103 94


Samples were spiked with high concentrations of Human NGF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P01138
SynonymsBeta-NGF, HSAN5, NGFB, NGF-B
Research AreaCancer, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


  1. Sport Sciences for Health (2022)
    The effects of aerobic exercise and sertraline on pro-inflammatory indices and amelioration of neurotrophic factors in patients who have undergone CABG

    DOI: 10.1007/s11332-022-00992-3

    Sample: Plasma,Serum
    The profile of urinary biomarkers in overactive bladder

    DOI: 10.1002/nau.24487

    PMID: 32813897

    Sample: Urine
    Increased levels of nerve growth factor accompany oxidative load in recurrent pregnancy loss. Machine learning applied to FT-Raman spectra study

    DOI: 10.1007/s00449-023-02847-8

    Sample: Serum
  4. American Journal of Translational Research (2021) IF: 4.06
    Effect of levetiracetam in combination with topiramate on immune function, cognitive function, and neuronal nutritional status of children with intractable epilepsy

    PMID: 34650715

    Sample: Serum
  5. Scientific Reports (2018) IF: 4.122
    Human Wharton’s jelly mesenchymal stem cells protect axotomized rat retinal ganglion cells via secretion of anti-inflammatory and neurotrophic factors

    DOI: 10.1038/s41598-018-34527-z

    Sample: Tissue Homogenate,Cell Lysate
  6. Stem Cell Research & Therapy (2017) IF: 4.211
    Adult human periodontal ligament-derived stem cells delay retinal degeneration and maintain retinal function in RCS rats

    DOI: 10.1186/s13287-017-0731-y

    Sample: Human Periodontal Ligament-Derived Stem Cells (Hpd
  7. Nutrients (2020) IF: 4.546
    The Effects of Tocotrienol-Rich Vitamin E (Tocovid) on Diabetic Neuropathy: A Phase II Randomized Controlled Trial

    DOI: 10.3390/nu12051522

    PMID: 32456230

    Sample: Serum
    The role of BDNF and NGF plasma levels in first-episode schizophrenia: A longitudinal study

    DOI: 10.1016/j.euroneuro.2022.02.003

    PMID: 35219096

    Sample: Plasma
    Single injection of a novel nerve growth factor coacervate improves structural and functional regeneration after sciatic nerve injury in adult rats

    DOI: 10.1016/j.expneurol.2016.10.015

    Sample: Others
  10. Journal of Functional Foods (2022) IF: 5.223
    Effects of erinacine A-enriched Hericium erinaceus on elderly hearing-impaired patients: A double-blind, randomized, placebo-controlled clinical trial

    DOI: 10.1016/j.jff.2022.105220

    Sample: Serum
    Dual Delivery of bFGF- and NGF-Binding Coacervate Confers Neuroprotection by Promoting Neuronal Proliferation

    DOI: 10.1159/000490139

    Sample: Serum
  12. Cell Metabolism (2022) IF: 31.373
    Cancer cells co-opt nociceptive nerves to thrive in nutrient-poor environments and upon nutrient-starvation therapies

    DOI: 10.1016/j.cmet.2022.10.012

    PMID: 36395769

    Sample: Plasma
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  • Q&A

F*******aSubmitted [ May 02 2023 ]

Asked: is there cross reactivity with MOUSE NGF?



adminSubmitted [ May 02 2023 ]

Answered: Dear Francesca, thanks for reaching out! I'll reply to you by email. Pls pay attention to it.

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