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Human NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) ELISA Kit

  • Cat.No.:E-EL-H0753

  • Reactivity: Human

To Purchase E-EL-H0753

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 78.13-5000 pg/mL
Sensitivity 46.88 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human NOS2/iNOS in samples. No significant cross-reactivity or interference between Human NOS2/iNOS and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human NOS2/iNOS concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NOS2/iNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NOS2/iNOS. You can calculate the concentration of Human NOS2/iNOS in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human NOS2/iNOS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human NOS2/iNOS were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 276.20 441.40 2054.00 265.80 481.50 2256.90
Standard deviation 14.10 24.70 71.90 17.30 26.50 92.50
CV (%) 5.10 5.60 3.50 6.51 5.50 4.10

Recovery

The recovery of Human NOS2/iNOS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-108 101
EDTA plasma (n=8) 94-107 99
Cell culture media (n=8) 85-95 90

Linearity

Samples were spiked with high concentrations of Human NOS2/iNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P35228
SynonymsNOS2A, HEP-NOS, I-NOS

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. International Journal of Cardiology (2016) IF: 6.189
    Melatonin Effects On Myocardial Ischemia-Reperfusion Injury: Impact On The Outcome In Patients Undergoing Coronary Artery Bypass Grafting Surgery.

    DOI: 10.1016/j.ijcard.2016.07.108

    PMID: 27441478

    Sample: Plasma
  2. The Journal of Infectious Diseases (2017) IF: 6.273
    Influence Of Leishmania RNA Virus-1 On Pro-inflammatory Biomarker Expression In A Human Macrophage Model Of American Tegumentary Leishmaniasis.

    DOI: 10.1093/infdis/jix416

    PMID: 28968786

    Sample: Cell culture supernatant
  3. International Journal of Cardiology (2016) IF: 6.189
    Melatonin Effects On Myocardial Ischemia-Reperfusion Injury: Impact On The Outcome In Patients Undergoing Coronary Artery Bypass Grafting Surgery.

    DOI: 10.1016/j.ijcard.2016.07.108

    PMID: 27441478

    Sample: Plasma
  4. Neuropsychiatry (London) (2017) IF: 4.778
    Plasma Pregnancy-Associated Plasma Protein A And CD40 Are Potential Biomarkers Of Vulnerable Plaque Associated With Middle Cerebral Artery Stenosis.
    Sample: Plasma
  5. Journal of Immunology Research (2016) IF: 3.276
    Monocyte-Derived Macrophages Are Impaired In Myelodysplastic Syndrome.

    DOI: 10.1155/2016/5479013

    PMID: 28074192

    Sample: Cell culture supernatant
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