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Human OC/BGP(Osteocalcin) ELISA Kit

  • Cat.No.:E-EL-H1343

  • Reactivity: Human

To Purchase E-EL-H1343

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 1.25-80 ng/mL
Sensitivity 0.75 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human OC/BGP in samples. No significant cross-reactivity or interference between Human OC/BGP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human OC/BGP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OC/BGP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OC/BGP. You can calculate the concentration of Human OC/BGP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human OC/BGP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human OC/BGP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 3.70 9.20 32.90 3.80 9.50 36.10
Standard deviation 0.20 0.40 1.60 0.30 0.50 1.30
CV (%) 5.41 4.35 4.86 7.89 5.26 3.60

Recovery

The recovery of Human OC/BGP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-109 99
EDTA plasma (n=8) 93-109 99
Cell culture media (n=8) 85-97 90

Linearity

Samples were spiked with high concentrations of Human OC/BGP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P02818
SynonymsBGLAP, BGP, OC, OCN, bone gamma-carboxyglutamate protein, Osteocalcin

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Materials Science and Engineering: C (2020) IF: 5.88
    Hyaluronic acid/corn silk extract based injectable nanocomposite: A biomimetic antibacterial scaffold for bone tissue regeneration

    DOI: 10.1016/j.msec.2019.110195

    PMID: 31761207

    Sample: Cell culture supernatant
  2. Bone (2019) IF: 4.455
    Daily oligofructose-enriched inulin intake impacts bone turnover markers T but not the cytokine profile in pediatric patients with celiac disease on a gluten-free diet: Results of a randomised, placebo-controlled pilot study

    DOI: 10.1016/j.bone.2019.03.001

    PMID: 30840918

    Sample: Plasma
  3. Bone (2018) IF: 4.455
    Bone and plasma citrate is reduced in osteoporosis.

    DOI: 10.1016/j.bone.2018.06.014

    PMID: 29929041

    Sample: serum
  4. Osteoporosis International (2018) IF: 3.864
    Vitamin D supplementation and bone turnover in advanced heart failure: the EVITA trial

    DOI: 10.1007/s00198-017-4312-9

    PMID: 29260292

    Sample: Plasma
  5. International journal of biological macromolecules (2019) IF: 3.909
    Bioactive fish scale incorporated chitosan biocomposite scaffolds for bone tissue engineering

    DOI: 10.1016/j.ijbiomac.2019.02.067

    PMID: 30797008

    Sample: Cell culture medium
  6. Journal of Cellular Biochemistry (2017) IF: 3.085
    In Vivo And In Vitro Effects Of PTH1-34 On Osteogenic And Adipogenic Differentiation Of Human Bone Marrow-Derived Mesenchymal Stem Cells Through Regulating MicroRNA-155.

    DOI: 10.1002/jcb.26478

    PMID: 29091308

    Sample: Cell lysate
  7. Journal of biomaterials applications (2019) IF: 2.082
    Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite

    DOI: 10.1177/0885328218811040

    PMID: 30451067

    Sample: Cell culture medium
  8. Hematology (2019) IF: 1.315
    Disordered bone metabolism in hereditary spherocytosis patients

    DOI: 10.1080/16078454.2019.1565150

    PMID: 31793406

    Sample: Serum
  9. Materials Science and Engineering: C (2018) IF: 1.793
    Biosilica incorporated 3D porous scaffolds for bone tissue engineering applications.

    DOI: 10.1016/j.msec.2018.05.040

    PMID: 30033256

    Sample: Serum
  10. Brazilian Journal of Medical and Biological Research (2016) IF: 1.578
    Effect Of Magnesium Ion On Human Osteoblast Activity.

    DOI: 10.1590/1414-431X20165257

    PMID: 27383121

    Sample: Cell culture supernatant
  11. Brazilian Journal of Medical and Biological Research (2015) IF: 1.578
    Neuropeptide Y, Substance P, And Human Bone Morphogenetic Protein 2 Stimulate Human Osteoblast Osteogenic Activity By Enhancing Gap Junction Intercellular Communication.

    DOI: 10.1590/1414-431X20144226

    PMID: 25714881

    Sample: Cell culture supernatant
  12. Journal of Advances in Medicine and Medical Research (2016)
    Evaluation of Osteopontin in Combination with Bone Turnover Markers for the Assessment of Osteoporosis in Postmenopausal Women

    DOI: 10.9734/BJMMR/2016/24738

    PMID: 99999999

    Sample: Serum
  13. Journal of Dental Sciences (2019) IF: 0.619
    Diagnostic accuracy of salivary biomarkers of bone turnover in identifying patients with periodontitis in a Saudi Arabian population

    DOI: 10.1016/j.jds.2019.03.002

    PMID: 31534637

    Sample: Saliva
  14. Materials Science and Engineering: C (2019)
    Bioactive diatomite and POSS silica cage reinforced chitosan/Na-carboxymethyl cellulose polyelectrolyte scaffolds for hard tissue regeneration

    DOI: 10.1016/j.msec.2019.02.104

    PMID: 30948053

    Sample: Cell culture supernatant
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