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Human OPG(Osteoprotegerin) ELISA Kit

  • Cat.No.:E-EL-H1341

  • Reactivity: Human

To Purchase E-EL-H1341

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human OPG in samples. No significant cross-reactivity or interference between Human OPG and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human OPG concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OPG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OPG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OPG. You can calculate the concentration of Human OPG in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human OPG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human OPG were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 1.20 5.00 0.50 1.10 4.80
Standard deviation 0.03 0.06 0.18 0.03 0.05 0.17
CV (%) 6.00 5.00 3.60 6.00 4.55 3.54


The recovery of Human OPG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-111 101
EDTA plasma (n=8) 96-110 102
Cell culture media (n=8) 86-101 93


Samples were spiked with high concentrations of Human OPG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   O00300
SynonymsTNFRSF11B , OCIF, TR1
Research AreaCancer, Cardiovascular, Metabolism, Immunology, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


  1. Oncotarget (2017) IF: 5.168
    Mesenchymal stem cells inhibit RANK-RANKL interactions between osteoclasts and Th17 cells via osteoprotegerin activity

    DOI: 10.18632/oncotarget.21379

    Sample: BM-derived Mesenchymal stem cells(BM-MSCs),adipose
  2. Frontiers in Pharmacology (2022) IF: 5.811
    Effect of Ibandronate Therapy on Serum Chemerin, Vaspin, Omentin-1 and Osteoprotegerin (OPG) in Postmenopausal Osteoporotic Females.

    DOI: 10.3389/fphar.2022.822671

    Sample: serum
  3. Scientific Reports (2017) IF: 4.259
    RNA sequencing reveals a transcriptomic portrait of human mesenchymal stem cells from bone marrow, adipose tissue, and palatine tonsils

    DOI: 10.1038/s41598-017-16788-2

    Sample: Cell culture supernatant
  4. World Journal of Stem Cells (2020) IF: 3.231
    CR6-interacting factor-1 contributes to osteoclastogenesis by inducing receptor activator of nuclear factor κB ligand after radiation

    DOI: 10.4252/wjsc.v12.i3.222

    Sample: H-BM-MSCs
  5. Journal of Immunology Research (2019) IF: 3.404
    Assessment of Salivary Levels of RANKL and OPG in Aggressive versus Chronic Periodontitis

    DOI: 10.1155/2019/6195258

    Sample: saliva
  6. CYTOKINE (2018) IF: 3.514
    Evaluation of gingival crevicular fluid and peri-implant crevicular fluid levels of sclerostin, TWEAK, RANKL and OPG

    DOI: 10.1016/j.cyto.2018.10.021

    PMID: 30377055

    Sample: Gingival Crevicular Fluid
  7. JOURNAL OF ENDODONTICS (2019) IF: 2.833
    Effect of Calcium Hydroxide Alone or in Combination with Ibuprofen and Ciprofloxacin on Nuclear Factor Kappa B Ligand and Osteoprotegerin Level in Periapical Lesions: A Randomized Controlled Clinical Study

    DOI: 10.1016/j.joen.2019.09.011

    Sample: interstitial fluid
    OPG/RANK/RANKL Axis in Egyptian Children With Acute Lymphoblastic Leukemia After Maintenance Therapy: Relationship to Bone Mineral and Vitamin D Status

    DOI: 10.1097/MPH.0000000000002603

    Sample: serum
    Association of serum FGF-23, klotho, fetuin-A, osteopontin, osteoprotegerin and hs-CRP levels with coronary artery disease

    DOI: 10.1080/00365513.2020.1728786

    Sample: serum
  10. Journal of Critical Care Medicine (2021)
    Prognostic Value of Bone Formation and Resorption Proteins in Heterotopic Ossification in Critically-Ill Patients. a Single-Centre Study.

    DOI: 10.2478/jccm-2020-0046

    Sample: plasma
  11. Journal of the Endocrine Society (2019)
    Increase in Osteocalcin Following Testosterone Therapy in Men With Type 2 Diabetes and Subnormal Free Testosterone

    DOI: 10.1210/js.2018-00426

    PMID: 31403089

    Sample: Plasma
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