Current Position:Home>>Kits>>ELISA Kits>> Human PⅠNP(Procollagen Ⅰ N-Terminal Propeptide) ELISA Kit
Synonyms:P1NP, N-Propeptide Of Type I Procollagen, Procollagen I Amino Terminal Propeptide
Detection Range: 15.63~1000 pg/mL
Sensitivity: 9.38 pg/mL
This ELISA kit applies to the in vitro quantitative determination of Human PⅠNP concentrations in Serum, plasma and other biological fluids.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PⅠNP . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PⅠNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PⅠNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PⅠNP. You can calculate the concentration of Human PⅠNP in the samples by comparing the OD of the samples to the standard curve.
|Detection Range||15.63—1000 pg/mL|
|Sample Volume Required Per Well||100μL|
|Sample Type||Serum, plasma and other biological fluids|
This kit recognizes Human PⅠNP in samples. No significant cross-reactivity or interference between Human PⅠNP and analogues was observed.
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PⅠNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PⅠNP were tested on 3 different plates, 20 replicates in each plate.
|Intra-assay Precision||Inter-assay Precision|
|C V (%)||6.24||4.69||4.23||6.33||4.39||4.48|
The recovery of Human PⅠNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
|Sample Type||Range (%)||Average Recovery (%)|
|EDTA plasma (n=5)||88-101||94|
|Cell culture media (n=5)||89-103||95|
Samples were spiked with high concentrations of Human PⅠNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
|Serum (n=5)||EDTA plasma (n=5)||Cell culture media (n=5)|
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.
|Micro ELISA Plate(Dismountable)||8 wells ×12 strips||-20°C, 6 months|
|Reference Standard||2 vials|
|Concentrated Biotinylated Detection Ab (100×)||1 vial, 120 μL|
|Concentrated HRP Conjugate (100×)||1 vial, 120 μL||-20°C(shading light), 6 months|
|Reference Standard & Sample Diluent||1 vial, 20 mL||2-8°C, 6 months|
|Biotinylated Detection Ab Diluent||1 vial, 14 mL|
|HRP Conjugate Diluent||1 vial, 14 mL|
|Concentrated Wash Buffer (25×)||1 vial, 30 mL|
|Substrate Reagent||1 vial, 10 mL||2-8°C(shading light)|
|Stop Solution||1 vial, 10 mL||2-8°C|
|Plate Sealer||5 pieces|
|Product Description||1 copy|
|Certificate of Analysis||1 copy|
Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results
P***lSubmitted [ Aug 28 2018 ]
Asked: I did notice that the assay kit for P1NP (Procollagen type 1 N-terminal propeptide) reports a very low detection range: it only detects values between 15.63 - 1000 pg/mL, or otherwise could be stated as 0.015 - 1 ng/mL. However, Population normal ranges for serum P1NP are: 15 - 80 ng/mL (I\'ve attached a poster reporting those population norms; the associated reference is Jenkins et al. 2013, Bone 55:271-276). Has the company made a typing error on the assay kit insert and used incorrect units or is that the true very low limits of detection for that kit?
adminSubmitted [ Aug 28 2018 ]
Answered: Thanks for your question. Referred from our in-house data, the normal serum samples were diluted in 500 folds, and the serum PINP levels were 40-110ng/mL, which consistents with the reference values in publications. If the concentration of the target protein in the sample is out of the highest detection limit, it just needs to be diluted to fall in the detection range. We usually try highest sensitivity to make the kits more flexible, so the kit could meet different experiments requirements. Further inquiry, please tell us.