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Human PD-L1(Programmed Cell Death Protein 1 Ligand 1) ELISA Kit

  • Cat.No.:E-EL-H1547

  • Reactivity: Human

To Purchase E-EL-H1547

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human PD-L1 in samples. No significant cross-reactivity or interference between Human PD-L1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human PD-L1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PD-L1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PD-L1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PD-L1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PD-L1. You can calculate the concentration of Human PD-L1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PD-L1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PD-L1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.54 1.35 4.76 0.49 1.29 5.23
Standard deviation 0.03 0.08 0.17 0.03 0.05 0.18
CV (%) 5.56 5.93 3.57 6.12 3.88 3.44


The recovery of Human PD-L1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-111 102
EDTA plasma (n=8) 92-104 98
Cell culture media (n=8) 90-102 95


Samples were spiked with high concentrations of Human PD-L1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q9NZQ7
SynonymsCD274, B7-H1, PD-L1, PDCD1L1, PDL1, B7 homolog 1
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


  1. Acta Pharmaceutica Sinica B (2021) IF: 11.614
    Abrogation of HnRNP L enhances anti-PD-1 therapy efficacy via diminishing PD-L1 and promoting CD8+ T cell-mediated ferroptosis in castration-resistant prostate cancer

    DOI: 10.1016/j.apsb.2021.07.016

    Sample: PC3 cells,DU145 cells
    Microfluidic-based exosome isolation and highly sensitive aptamer exosome membrane protein detection for lung cancer diagnosis

    DOI: 10.1016/j.bios.2022.114487

    PMID: 35780540

    Sample: exosomes
  3. Frontiers in Immunology (2021) IF: 7.561
    PD-L1 Dysregulation in COVID-19 Patients

    DOI: 10.3389/fimmu.2021.695242

    Sample: serum
  4. Biomed Research International (2021) IF: 3.411
    Relationship of PD-1 (PDCD1) and PD-L1 (CD274) Single Nucleotide Polymorphisms with Polycystic Ovary Syndrome

    DOI: 10.1155/2021/9596358

    PMID: 33521133

    Sample: Serum
    Serum sPD‐L1, Upregulated in Sepsis, May Reflect Disease Severity and Clinical Outcomes in Septic Patients

    DOI: 10.1111/sji.12509

    Sample: Serum
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