Human PDGF-BB(Platelet Derived Growth Factor BB) ELISA Kit

    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
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    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-H1577

      Synonyms:PDGFBB

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $609

      Reactivity: Human

      Detection Range: 31.25~2000 pg/mL

      Sensitivity: 18.75 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Human PDGF-BB concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDGF-BB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDGF-BB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDGF-BB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PDGF-BB. You can calculate the concentration of Human PDGF-BB in the samples by comparing the OD of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Human
      Detection Method Colormetric
      Detection Range 31.25—2000 pg/mL
      Sensitivity 18.75 pg/mL
      Sample Volume Required Per Well 100μL
      Sample Type Serum, plasma and other biological fluids

      Specificity

      This kit recognizes Human PDGF-BB in samples. No significant cross-reactivity or interference between Human PDGF-BB and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration
      (pg/mL)
      O.D Average Corrected
      2000 2.523
      2.559
      2.541 2.456
      1000 1.615
      1.659
      1.637 1.552
      500 0.958
      0.942
      0.95 0.865
      250 0.458
      0.468
      0.463 0.378
      125 0.254
      0.254
      0.254 0.169
      62.5 0.188
      0.186
      0.187 0.102
      31.25 0.128
      0.146
      0.137 0.052
      0 0.081
      0.089
      0.085 --

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PDGF-BB were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PDGF-BB were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (pg/mL) 108.61 262.35 849.02 105.51 269.64 922.05
      Standard deviation 7.16 12.80 34.56 6.90 13.00 39.37
      C V (%) 6.59 4.88 4.07 6.54 4.82 4.27

      Recovery

      The recovery of Human PDGF-BB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 95-108 102
      EDTA plasma (n=5) 85-101 92
      Cell culture media (n=5) 87-100 93

      Linearity

      Samples were spiked with high concentrations of Human PDGF-BB and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 93-106 85-97 89-103
      Average (%) 100 91 96
      1:4 Range (%) 87-99 80-91 89-101
      Average (%) 93 85 95
      1:8 Range (%) 86-99 83-97 89-101
      Average (%) 93 90 94
      1:16 Range (%) 89-105 86-102 83-97
      Average (%) 96 93 90

      Kit components & Storage

      An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20°C, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20°C(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 2-8°C(shading light)
      Stop Solution 1 vial, 10 mL 2-8°C
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37°C
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

      elisa assay procedure 2

      2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

      elisa assay procedure 3

      3. Aspirate and wash the plate for 3 times

      elisa assay procedure 4

      4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

      elisa assay procedure 5

      5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

      elisa assay procedure 6

      6. Add 50μL Stop Solution

      elisa assay procedure 7

      7. Read the plate at 450nm immediately. Calculation of the results

      Citations

      1. Leukocyte Depletion Does Not Affect The In Vitro Healing Ability Of Platelet Rich Plasma.
        Journal: Experimental and Therapeutic Medicine(2018)
        DOI: 10.3892/etm.2018.5887
        PMID: 29556269
        Products: E-EL-H0108 E-EL-H1577 E-EL-H1575 E-EL-H1670 E-EL-0162 E-EL-H0109 E-EL-H0092 
        Reactivity: Human
      2. Growth factor release and enhanced encapsulated periodontal stem cells viability by freeze-dried platelet concentrate loaded thermo-sensitive hydrogel for periodontal regeneration.
        IF: /
        Journal: The Saudi Dental Journal(2018)
        DOI: 10.1016/j.sdentj.2018.06.002
        PMID: 30202174
        Products: E-EL-H1577 
        Sample type: FDPC
        Reactivity: Human
      3. A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility
        IF: 6.638
        Journal: Acta Biomaterialia(2019)
        DOI: 10.1016/j.actbio.2019.05.012
        PMID: 31075515
        Products: E-EL-H0111 E-EL-0162 E-EL-H1577 
        Sample type: Cell culture supernatant
        Reactivity: Human
      4. A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility
        IF: 6.638
        Products: E-EL-H0111 E-EL-H0110 E-EL-H1577 
        Sample type: Cell supernatant
        Reactivity: Human
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