Current Position:Home>>Kits>>ELISA Kits>> Human PⅢNP(N-Terminal Procollagen Ⅲ Propeptide) ELISA Kit
Cat.No.:E-EL-H0183
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 23.44-1500 pg/mL |
Sensitivity | 14.06 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human PⅢNP in samples. No significant cross-reactivity or interference between Human PⅢNP and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human PⅢNP concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PⅢNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PⅢNP were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 75.40 | 208.10 | 518.30 | 69.80 | 205.90 | 509.50 |
Standard deviation | 4.80 | 11.40 | 18.70 | 3.90 | 9.90 | 18.30 |
CV (%) | 6.37 | 5.48 | 3.61 | 5.59 | 4.81 | 3.59 |
The recovery of Human PⅢNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 95-107 | 101 |
EDTA plasma (n=8) | 96-107 | 102 |
Cell culture media (n=8) | 85-99 | 92 |
Samples were spiked with high concentrations of Human PⅢNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | P3NP, N-Propeptide Of Type III Procollagen, Procollagen III Amino Terminal Propeptide |
Research Area | Signal Transduction |
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1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
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Sample: Serum |
PMID: 32036784 |
Sample: Whole Blood |
PMID: 36040510 |
Sample: serum |
Sample: Serum |
PMID: 33730073 |
Sample: Cell culture supernatant |
Sample: Serum |
PMID: 31528100 |
Sample: Serum |
DOI: 10.1002/ehf2.14186 |
|
PMID: 36303443 |
Sample: Cardiac fibroblasts |
PMID: 35741245 |
Sample: serum |
PMID: 30535520 |
Sample: Serum |
DOI: 10.1002/dmrr.2998 |
|
Sample: Serum |
Sample: serum |
Sample: Serum |
PMID: 31377918 |
Sample: Serum |
Sample: Serum |
Sample: serum |
DOI: 10.1159/000499932 |
|
Sample: serum |
A*******aSubmitted [ Apr 19 2021 ]
Asked: Can we get coated plates separately for P3NP kit (cat.no. E-EL-H0183)
Reply
adminSubmitted [ Apr 19 2021 ]
Answered: Dear Aishwarya, Thanks for your inquiry. I just have replied to your email box, pls kindly check it out. Thank you and best regards.