Human PⅢNP(N-Terminal Procollagen Ⅲ Propeptide) ELISA Kit

  • Cat.No.:E-EL-H0183

  • Reactivity: Human

To Purchase E-EL-H0183

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 23.44-1500 pg/mL
Sensitivity 14.06 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human PⅢNP in samples. No significant cross-reactivity or interference between Human PⅢNP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human PⅢNP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PⅢNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PⅢNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PⅢNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PⅢNP. You can calculate the concentration of Human PⅢNP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PⅢNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PⅢNP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 75.40 208.10 518.30 69.80 205.90 509.50
Standard deviation 4.80 11.40 18.70 3.90 9.90 18.30
CV (%) 6.37 5.48 3.61 5.59 4.81 3.59


The recovery of Human PⅢNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-107 101
EDTA plasma (n=8) 96-107 102
Cell culture media (n=8) 85-99 92


Samples were spiked with high concentrations of Human PⅢNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsP3NP, N-Propeptide Of Type Ⅲ Procollagen, Procollagen Ⅲ Amino Terminal Propeptide

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Diabetes/Metabolism Research And Reviews (2018) IF: 8.6839
    Circulating PIIINP Levels Identify Adipose Tissue‐Associated Inflammation In Individuals With Type 2 Diabetes Mellitus With And Without Non‐Alcoholic Fatty Liver Disease.

    DOI: 10.1002/dmrr.2998

    PMID: 29471595

    Sample: Serum
  2. BMC Medicine (2016) IF: 8.097
    No Effects Of Oral Vitamin D Supplementation On Non-Alcoholic Fatty Liver Disease In Patients With Type 2 Diabetes: A Randomized, Double-Blind, Placebo-Controlled Trial.

    DOI: 10.1186/s12916-016-0638-y

    PMID: 27353492

    Sample: Serum
  3. Journal of Cardiovascular Magnetic Resonance (2020) IF: 5.361
    Imaging, biomarker and invasive assessment of diffuse left ventricular myocardial fibrosis in atrial fibrillation

    DOI: 10.1186/s12968-020-0603-y

    PMID: 32036784

    Sample: Whole Blood
  4. Osteoarthritis and Cartilage (2017) IF: 4.742
    Associations Between Serum Ghrelin And Knee Symptoms, Joint Structures And Cartilage Or Bone Biomarkers In Patients With Knee Osteoarthritis.

    DOI: 10.1016/j.joca.2017.05.015

    PMID: 28602782

    Sample: Serum
  5. World journal of gastroenterology (2019) IF: 3.665
    On-treatment Monitoring of Liver Fibrosis With Serum Hepatitis B Core-Related Antigen in Chronic Hepatitis B

    DOI: 10.3748/wjg.v25.i32.4764

    PMID: 31528100

    Sample: Serum
  6. Frontiers in Physiology (2020) IF: 3.201
    Elevated b1-Adrenergic Receptor Autoantibody Levels Increase Atrial Fibrillation Susceptibility by Promoting Atrial Fibrosis

    DOI: 10.3389/fphys.2020.00076

    PMID: 32116783

    Sample: Serum
  7. Annals of intensive care (2018) IF: 3.771
    Driving pressure and long-term outcomes in moderate/severe acute respiratory distress syndrome

    DOI: 10.1186/s13613-018-0469-4

    PMID: 30535520

    Sample: Serum
  8. Heart and Vessels (2015) IF: 3.434
    Extracellular Matrix Turnover In Coronary Artery Ectasia Patients.

    DOI: 10.1007/s00380-014-0622-4

    PMID: 25576491

    Sample: Serum
  9. Clinical Rheumatology (2019) IF: 2.394
    Associations between serum IL-8 and knee symptoms, joint structures, and cartilage or bone biomarkers in patients with knee osteoarthritis

    DOI: 10.1007/s10067-019-04718-8

    PMID: 31377918

    Sample: Serum
  10. Clinical Biochemistry (2016) IF: 2.434
    Circulating Biomarkers Of Fibrosis And Cardioversion Of Atrial Fibrillation: A Prospective, Controlled Cohort Study.

    DOI: 10.1016/j.clinbiochem.2016.09.008

    PMID: 27622867

    Sample: Serum
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  • Q&A

A*******aSubmitted [ Apr 19 2021 ]

Asked: Can we get coated plates separately for P3NP kit (cat.no. E-EL-H0183)



adminSubmitted [ Apr 19 2021 ]

Answered: Dear Aishwarya, Thanks for your inquiry. I just have replied to your email box, pls kindly check it out. Thank you and best regards.

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