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Human PLAP/ALPP(Placental Alkaline Phosphatase) ELISA Kit

  • Cat.No.:E-EL-H1976

  • Reactivity: Human

To Purchase E-EL-H1976

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human PLAP/ALPP in samples. No significant cross-reactivity or interference between Human PLAP/ALPP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human PLAP/ALPP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PLAP/ALPP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PLAP/ALPP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLAP/ALPP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PLAP/ALPP. You can calculate the concentration of Human PLAP/ALPP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PLAP/ALPP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PLAP/ALPP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.52 1.03 3.53 0.56 1.04 3.68
Standard deviation 0.03 0.06 0.12 0.04 0.04 0.13
CV (%) 5.77 5.83 3.40 7.14 3.85 3.53

Recovery

The recovery of Human PLAP/ALPP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-103 97
EDTA plasma (n=8) 86-98 93
Cell culture media (n=8) 93-108 99

Linearity

Samples were spiked with high concentrations of Human PLAP/ALPP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P05187
SynonymsALPP, ALP, PALP, PLAP, PLAP-1, alkaline phosphatase, placental

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. International journal of nanomedicine (2019) IF: 5.115
    Exosomal microRNA profiling in early and late onset preeclamptic pregnant women reflects pathophysiology

    DOI: 10.2147/IJN.S208865

    PMID: 31413567

    Sample: Plasma
  2. Cytokine (2020) IF: 2.952
    Exosomal Th1/Th2 cytokines in preeclampsia and HIV-positive preeclamptic women on highly active anti-retroviral therapy

    DOI: 10.1016/j.cyto.2019.154795

    PMID: 31398625

    Sample: Serum
  3. Placenta (2016) IF: 2.759
    Placental Exosomes And Pre-Eclampsia: Maternal Circulating Levels In Normal Pregnancies And, Early And Late Onset Pre-Eclamptic Pregnancies.

    DOI: 10.1016/j.placenta.2016.08.078

    PMID: 27697217

    Sample: Plasma
  4. European Journal of Obstetrics & Gynecology and Reproductive Biology (2020) IF: 1.868
    The impact of circulating exosomes derived from early and late onset pre-eclamptic pregnancies on inflammatory cytokine secretion by BeWo cells

    DOI: 10.1016/j.ejogrb.2020.02.032

    PMID: 32114320

    Sample: Plasma
  5. European Journal of Obstetrics & Gynecology And Reproductive Biology (2018) IF: 1.666
    Placental Hypoxia Inducible Factor-1α & CHOP Immuno-Histochemical Expression Relative To Maternal Circulatory Syncytiotrophoblast Micro-vesicles In Preeclamptic And Normotensive Pregnancies.

    DOI: 10.1016/j.ejogrb.2017.11.004

    PMID: 29127866

    Sample: Microvesicle
  6. Journal of Maternal-Fetal & Neonatal Medicine (2017) IF: 1.826
    Serum Endothelin-1 And Placental Alkaline Phosphatase Levels In Placenta Percreta And Normal Pregnancies.

    DOI: 10.1080/14767058.2017.1297412

    PMID: 28274166

    Sample: Serum
  7. INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY (2016)
    IMPACT OF SERUM PLACENTAL ALKALINE PHOSPHATASE (PALP) LEVEL ON ABORTION

    PMID: 99999999

    Sample: Serum
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