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Human PON1(Paraoxonase 1) ELISA Kit

Citations(5)Uniprot : P27169

Cat.No.:E-EL-H2298
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human PON1 in samples. No significant cross-reactivity or interference between Human PON1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human PON1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PON1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PON1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PON1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PON1. You can calculate the concentration of Human PON1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PON1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PON1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.46	1.53	4.72	0.45	1.47	4.63
Standard deviation	0.03	0.06	0.26	0.03	0.06	0.19
CV (%)	6.52	3.92	5.51	6.67	4.08	4.10

Recovery

The recovery of Human PON1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-105	98
EDTA plasma (n=8)	86-99	91
Cell culture media (n=8)	95-108	100

Linearity

Samples were spiked with high concentrations of Human PON1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P27169
Synonyms	ESA, MVCD5, Aromatic Esterase 1, Serum Aryldialkylphosphatase 1
Research Area	Cancer, Cell Biology, Cardiovascular, Metabolism, Neuroscience, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Annals of vascular surgery (2020) IF: 1.125

Paraoxonase-1 and Symptomatic Status in Carotid Artery Disease

DOI: 10.1016/j.avsg.2019.07.020
PMID: 31626928	Sample: Serum

EurAsian Journal of BioSciences (2019)

A medical bioscience study of serum levels of paraoxonase 1 activity and total antioxidant capacity relationship with anti-mullerian hormone in polycystic ovary syndrome


	Sample: Serum

Eurasian Journal of Biosciences (2019)

Serum levels of Paraoxonase 1 activity and total antioxidant capacity relationship with anti-mullerian hormone in polycystic ovary syndrome


	Sample: Serum

Pakistan Journal of Medical Sciences (2019) IF: 0.754

Correlation of Paraoxonase-1 with glycated hemoglobin and lipid profile among Sudanese diabetic patients

DOI: 10.12669/pjms.35.4.26
PMID: 31372141	Sample: Serum

Meta Gene (2018) IF: /

Association of cholesteryl ester transferyl protein gene (G277A) polymorphism and risk prediction of type 2 diabetes mellitus: A case control study of Northern Indian population.

DOI: 10.1016/j.mgene.2018.06.005
	Sample: Serum

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