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Human RETN(Resistin) ELISA Kit

  • Cat.No.:E-EL-H1213

  • Reactivity: Human

To Purchase E-EL-H1213

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human RETN in samples. No significant cross-reactivity or interference between Human RETN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human RETN concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RETN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RETN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RETN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RETN. You can calculate the concentration of Human RETN in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human RETN were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human RETN were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 104.60 212.00 977.10 112.00 217.50 915.30
Standard deviation 7.10 11.90 44.90 7.70 12.20 33.90
CV (%) 6.79 5.61 4.60 6.88 5.61 3.70


The recovery of Human RETN spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-102 96
EDTA plasma (n=8) 87-101 93
Cell culture media (n=8) 91-107 98


Samples were spiked with high concentrations of Human RETN and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q9HD89
Research AreaCardiovascular, Metabolism, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Frontiers in Endocrinology (2022) IF: 6.055
    Serum Resistin Levels and Related Genetic Variants Are Associated With Bone Mineral Density in Postmenopausal Women

    DOI: 10.3389/fendo.2022.868120

    PMID: 35992125

    Sample: serum
  2. Oncotarget (2016) IF: 5.008
    Adipocytes secreted leptin is a pro-tumor factor for survival of multiple myeloma under chemotherapy

    DOI: 10.18632/oncotarget.13342

    Sample: plasma
    Identification of key candidate biomarkers for severe influenza infection by integrated bioinformatical analysis and initial clinical validation

    DOI: 10.1111/jcmm.16275

    PMID: 33448094

    Sample: Plasma
  4. Frontiers in Endocrinology (2021) IF: 5.555
    Association Between Vitamin D and Resistin in Postmenopausal Females With Altered Bone Health

    DOI: 10.3389/fendo.2020.615440

    Sample: Serum
  5. Scientific Reports (2021) IF: 4.38
    Obesity-related proteins score as a potential marker of breast cancer risk

    DOI: 10.1038/s41598-021-87583-3

    PMID: 33859244

    Sample: Plasma
    Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals

    DOI: 10.1016/j.diabres.2019.05.023

    PMID: 31150725

    Sample: Serum
  7. JOURNAL OF NEPHROLOGY (2021) IF: 3.902
    Body composition, adipokines, FGF23-Klotho and bone in kidney transplantation: Is there a link?

    DOI: 10.1007/s40620-021-00972-9

    PMID: 33560479

    Sample: Serum
    Circadian rhythm abnormalities in patients with inflammatory bowel disease – association with adipokine profile

    DOI: 10.1080/00365521.2020.1737727

    Sample: Serum
  9. Obesity Research & Clinical Practice (2022) IF: 2.288
    The effect of omentectomy on the blood levels of adipokines in obese patients with endometrial cancer

    DOI: 10.1016/j.orcp.2022.06.002

    PMID: 35701296

    Sample: serum
    Resistin is not a useful insulin resistance marker for non-obese patients.

    DOI: 10.26402/jpp.2020.3.06

    Sample: blood
  11. Lipids in Health and Disease (2019) IF: 2.651
    Leptin, resistin and visfatin as useful predictors of gestational diabetes mellitus

    DOI: 10.1186/s12944-019-1169-2

    PMID: 31836012

    Sample: Serum
  12. Endokrynologia Polska (2021) IF: 1.582
    New insights into the metabolic-bone crosstalk in active acromegaly

    DOI: 10.5603/EP.a2021.0028

    Sample: serum
  13. Clinical Hypertension (2020)
    Serum adipocytokines and adiposity as predictive indices of preeclampsia

    DOI: 10.1186/s40885-020-00152-0

    PMID: 33014422

    Sample: Serum,Urine
  14. Health Care for Women International (2020) IF: 0.97
    Serum resistin levels as predictor of low bone mineral density in postmenopausal women

    DOI: 10.1080/07399332.2020.1798965

    Sample: serum
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