Click here to view cell apoptosis assay products

Human S100B(S100 Calcium Binding Protein B) ELISA Kit

  • Cat.No.:E-EL-H1297

  • Reactivity: Human

To Purchase E-EL-H1297

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human S100B in samples. No significant cross-reactivity or interference between Human S100B and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human S100B concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human S100B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human S100B. You can calculate the concentration of Human S100B in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human S100B were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human S100B were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 104.40 168.30 729.10 107.50 175.90 681.00
Standard deviation 7.10 7.70 25.50 5.80 8.30 32.00
CV (%) 6.80 4.58 3.50 5.40 4.72 4.70


The recovery of Human S100B spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-98 93
EDTA plasma (n=8) 93-108 100
Cell culture media (n=8) 95-112 102


Samples were spiked with high concentrations of Human S100B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P04271
SynonymsS100-B, NEF, S100Beta
Research AreaCancer, Cell Biology, Epigenetics and Nuclear Signaling, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Nutrients (2021) IF: 5.719
    Rapid and Effective Vitamin D Supplementation May Present Better Clinical Outcomes in COVID-19 (SARS-CoV-2) Patients by Altering Serum INOS1, IL1B, IFNg, Cathelicidin-LL37, and ICAM1

    DOI: 10.3390/nu13114047

    PMID: 34836309

    Sample: serum
  2. Frontiers in Neurology (2022) IF: 4.086
    Neurofilament light chain and S100B serum levels are associated with disease severity and outcome in patients with aneurysmal subarachnoid hemorrhage

    DOI: 10.3389/fneur.2022.956043

    PMID: 35989914

    Sample: serum
    Plasma biomarkers of brain injury in COVID-19 patients with neurological symptoms

    DOI: 10.1016/j.jns.2022.120324

    PMID: 35752131

    Sample: serum
    Short-Term Postoperative Cognitive Dysfunction and Inflammatory Response in Patients Undergoing Cytoreductive Surgery and Hyperthermic Intraperitoneal Chemotherapy: A Pilot Study

    DOI: 10.1155/2017/3605350

    Sample: Serum
    Can the Prognosis of COVID-19 Disease Be Determined by Fecal Markers and Cytokines?

    DOI: 10.1089/jir.2022.0098

    PMID: 36179036

    Sample: fecal
  6. BRAIN INJURY (2021) IF: 2.311
    Changes in amplitude-integrated electroencephalography, neuron-specific enolase, and S100B in neonates with brain injury induced by neonatal hyperbilirubinemia and their significance

    DOI: 10.1080/02699052.2021.1931449

    PMID: 34097553

    Sample: serum
    The elevation of S100B and downregulation of circulating miR-602 in the sera of ischemic stroke (IS) patients: the emergence of novel diagnostic and prognostic markers

    DOI: 10.1007/s10072-020-04323-7

    Sample: serum
    Association of BDNF levels with IQ: comparison of S100B and BDNF levels in typically developing children and subjects with neurologically normal nonsyndromic intellectual disability

    DOI: 10.1111/jir.12896

    PMID: 34750906

    Sample: Serum
  9. BMC Neurology (2022) IF: 2.474
    Changes of biochemical biomarkers in the serum of children with convulsion status epilepticus: a prospective study

    DOI: 10.1186/s12883-022-02686-2

    PMID: 35624413

    Sample: serum
    Enteroviral meningitis reduces CSF concentration of Aβ42, but does not affect markers of parenchymal damage

    DOI: 10.1007/s10096-019-03569-0

    Sample: cerebrospinal fluid(CSF)
  11. MEDICINE (2021) IF: 1.889
    Serum NSE and S100B protein levels for evaluating the impaired consciousness in patients with acute carbon monoxide poisoning

    DOI: 10.1097/MD.0000000000026458

    PMID: 34160445

    Sample: serum
  12. Advanced Pharmaceutical Bulletin (2021)
    Targeting Mitochondrial and Brain Injury Markers in Acquired Brain Injuries: A Randomized, Double-Blind, Placebo-Controlled Study with Melatonin

    DOI: 10.34172/apb.2022.013

    Sample: serum
Show AllShorten


  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for Free Shipping 24T ELISA Kit
*Product Name:
*Catalog Number:
*When will you use it?