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Human SDF-1/CXCL12(Stromal Cell Derived Factor 1) ELISA Kit

Citations(13)Uniprot : P48061

Cat.No.:E-EL-H0052
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human SDF-1/CXCL12 in samples. No significant cross-reactivity or interference between Human SDF-1/CXCL12 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human SDF-1/CXCL12 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SDF-1/CXCL12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SDF-1/CXCL12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SDF-1/CXCL12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SDF-1/CXCL12. You can calculate the concentration of Human SDF-1/CXCL12 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human SDF-1/CXCL12 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human SDF-1/CXCL12 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.50	1.40	3.60	0.50	1.50	3.60
Standard deviation	0.03	0.07	0.19	0.03	0.09	0.14
CV (%)	6.00	5.00	5.28	6.00	6.00	3.89

Recovery

The recovery of Human SDF-1/CXCL12 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-102	94
EDTA plasma (n=8)	88-101	93
Cell culture media (n=8)	91-102	96

Linearity

Samples were spiked with high concentrations of Human SDF-1/CXCL12 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P48061
Synonyms	SDF1, CXCL12, IRH, PBSF, SCYB12, TLSF, TPAR1
Research Area	Cancer, Cardiovascular, Immunology, Microbiology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Theranostics (2021) IF: 11.556
A SCID mouse-human lung xenograft model of SARS-CoV-2 infection

DOI: 10.7150/thno.58321

PMID: 33995679
Sample: Tissue homogenate
Cell Death & Disease (2020) IF: 8.4689
Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion

DOI: 10.1038/s41419-020-2665-z

PMID: 32532953
Sample: Cell culture supernatant
Acta Biomaterialia (2021) IF: 8.9469
Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury

DOI: 10.1016/j.actbio.2021.06.038

PMID: 34217903
Sample: BMSCs clls
Acta Biomaterialia (2018) IF: 8.9469
Multilayered coating of titanium implants promotes coupled osteogenesis and angiogenesis in vitro and in vivo

DOI: 10.1016/j.actbio.2018.04.043

Sample: human umbilical vein endothelial cells (HUVECs)
Pharmaceutics (2022) IF: 6.525
Anti-Fibrotic Effect of SDF-1β Overexpression in Bleomycin-Injured Rat Lung

DOI: 10.3390/pharmaceutics14091803

Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 6.53
Hypoxia-induced microRNA-141 regulates trophoblast apoptosis, invasion, and vascularization by blocking CXCL12β/CXCR2/4 signal transduction

DOI: 10.1016/j.biopha.2019.108836

Sample: HTR-8/SVneo cells
Journal of Inflammation Research (2022) IF: 6.922
Targeting CXCL12/CXCR4 Signaling with AMD3100 Might Selectively Suppress CXCR4+ T-Cell Chemotaxis Leading to the Alleviation of Chronic Prostatitis

DOI: 10.2147/JIR.S352336

PMID: 35479835
Sample: serum
NEPHROLOGY DIALYSIS TRANSPLANTATION (2021) IF: 5.992
Effect of fructooligosaccharide on endothelial function in CKD patients: a randomized controlled trial

DOI: 10.1093/ndt/gfaa335

Sample: serum
Endocrine Connections (2022) IF: 3.335
Association of serum stromal cell-derived factor-1 levels with EZSCAN score and its derived indicators in patients with type 2 diabetes

DOI: 10.1530/EC-21-0629

Sample: serum
ENDOCRINE JOURNAL (2021) IF: 2.349
Serum stromal cell-derived factor-1 levels are associated with diabetic kidney disease in type 2 diabetic patients

DOI: 10.1507/endocrj.EJ21-0039

PMID: 33896872
Sample: serum
PLoS One (2017) IF: 2.806
Depot-specific inflammation with decreased expression of ATM2 in white adipose tissues induced by high-margarine/lard intake

DOI: 10.1371/journal.pone.0188007

Sample: Cell culture supernatant
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION (2020) IF: 1.475
Extracellular ubiquitin levels are increased in coronary heart disease and associated with the severity of the disease

DOI: 10.1080/00365513.2020.1728783

PMID: 32077763
Sample: Plasma
THERAPEUTIC APHERESIS AND DIALYSIS (2021) IF: 1.762
Angiogenic markers predict kidney injury and obstetric complications in women with preeclampsia and pregnancy-related acute kidney injury

DOI: 10.1111/1744-9987.13633

PMID: 33533567
Sample: Serum

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