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Human SDF-1/CXCL12(Stromal Cell Derived Factor 1) ELISA Kit

  • Cat.No.:E-EL-H0052

  • Reactivity: Human

To Purchase E-EL-H0052

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human SDF-1/CXCL12 in samples. No significant cross-reactivity or interference between Human SDF-1/CXCL12 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human SDF-1/CXCL12 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SDF-1/CXCL12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SDF-1/CXCL12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SDF-1/CXCL12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SDF-1/CXCL12. You can calculate the concentration of Human SDF-1/CXCL12 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human SDF-1/CXCL12 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human SDF-1/CXCL12 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 1.40 3.60 0.50 1.50 3.60
Standard deviation 0.03 0.07 0.19 0.03 0.09 0.14
CV (%) 6.00 5.00 5.28 6.00 6.00 3.89

Recovery

The recovery of Human SDF-1/CXCL12 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-102 94
EDTA plasma (n=8) 88-101 93
Cell culture media (n=8) 91-102 96

Linearity

Samples were spiked with high concentrations of Human SDF-1/CXCL12 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P48061
SynonymsSDF1, CXCL12, IRH, PBSF, SCYB12, TLSF, TPAR1
Research AreaCancer, Cardiovascular, Immunology, Microbiology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Theranostics (2021) IF: 11.556
    A SCID mouse-human lung xenograft model of SARS-CoV-2 infection

    DOI: 10.7150/thno.58321

    PMID: 33995679

    Sample: Tissue homogenate
  2. Cell Death & Disease (2020) IF: 8.4689
    Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion

    DOI: 10.1038/s41419-020-2665-z

    PMID: 32532953

    Sample: Cell culture supernatant
  3. Acta Biomaterialia (2021) IF: 8.9469
    Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury

    DOI: 10.1016/j.actbio.2021.06.038

    PMID: 34217903

    Sample: BMSCs clls
  4. Acta Biomaterialia (2018) IF: 8.9469
    Multilayered coating of titanium implants promotes coupled osteogenesis and angiogenesis in vitro and in vivo

    DOI: 10.1016/j.actbio.2018.04.043

    Sample: human umbilical vein endothelial cells (HUVECs)
  5. Pharmaceutics (2022) IF: 6.525
    Anti-Fibrotic Effect of SDF-1β Overexpression in Bleomycin-Injured Rat Lung

    DOI: 10.3390/pharmaceutics14091803

    Sample: serum
  6. BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 6.53
    Hypoxia-induced microRNA-141 regulates trophoblast apoptosis, invasion, and vascularization by blocking CXCL12β/CXCR2/4 signal transduction

    DOI: 10.1016/j.biopha.2019.108836

    Sample: HTR-8/SVneo cells
  7. Journal of Inflammation Research (2022) IF: 6.922
    Targeting CXCL12/CXCR4 Signaling with AMD3100 Might Selectively Suppress CXCR4+ T-Cell Chemotaxis Leading to the Alleviation of Chronic Prostatitis

    DOI: 10.2147/JIR.S352336

    PMID: 35479835

    Sample: serum
  8. NEPHROLOGY DIALYSIS TRANSPLANTATION (2021) IF: 5.992
    Effect of fructooligosaccharide on endothelial function in CKD patients: a randomized controlled trial

    DOI: 10.1093/ndt/gfaa335

    Sample: serum
  9. Endocrine Connections (2022) IF: 3.335
    Association of serum stromal cell-derived factor-1 levels with EZSCAN score and its derived indicators in patients with type 2 diabetes

    DOI: 10.1530/EC-21-0629

    Sample: serum
  10. ENDOCRINE JOURNAL (2021) IF: 2.349
    Serum stromal cell-derived factor-1 levels are associated with diabetic kidney disease in type 2 diabetic patients

    DOI: 10.1507/endocrj.EJ21-0039

    PMID: 33896872

    Sample: serum
  11. PLoS One (2017) IF: 2.806
    Depot-specific inflammation with decreased expression of ATM2 in white adipose tissues induced by high-margarine/lard intake

    DOI: 10.1371/journal.pone.0188007

    Sample: Cell culture supernatant
  12. SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION (2020) IF: 1.475
    Extracellular ubiquitin levels are increased in coronary heart disease and associated with the severity of the disease

    DOI: 10.1080/00365513.2020.1728783

    PMID: 32077763

    Sample: Plasma
  13. THERAPEUTIC APHERESIS AND DIALYSIS (2021) IF: 1.762
    Angiogenic markers predict kidney injury and obstetric complications in women with preeclampsia and pregnancy-related acute kidney injury

    DOI: 10.1111/1744-9987.13633

    PMID: 33533567

    Sample: Serum
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