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Human SOST(Sclerostin) ELISA Kit

  • Cat.No.:E-EL-H1544

  • Reactivity: Human

To Purchase E-EL-H1544

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 62.50-4000 pg/mL
Sensitivity 37.50 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human SOST in samples. No significant cross-reactivity or interference between Human SOST and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human SOST concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SOST. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SOST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SOST, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SOST. You can calculate the concentration of Human SOST in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human SOST were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human SOST were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 222.11 370.69 1524.64 203.00 389.31 1557.60
Standard deviation 11.82 15.05 67.24 11.27 21.30 67.60
CV (%) 5.32 4.06 4.41 5.55 5.47 4.34


The recovery of Human SOST spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-99 91
EDTA plasma (n=8) 92-107 98
Cell culture media (n=8) 88-103 94


Samples were spiked with high concentrations of Human SOST and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q9BQB4
SynonymsCDD, VBCH
Research AreaCancer, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

    Evaluation of GCF IL-17, IL-10, TWEAK and sclerostin levels after SRP and adjunctive use of diode laser application in periodontitis patients

    DOI: 10.1002/JPER.21-0494

    Sample: gingival crevicular fluid (GCF)
  2. CYTOKINE (2018) IF: 3.514
    Evaluation of gingival crevicular fluid and peri-implant crevicular fluid levels of sclerostin, TWEAK, RANKL and OPG

    DOI: 10.1016/j.cyto.2018.10.021

    PMID: 30377055

    Sample: Gingival Crevicular Fluid
    Evaluation of serum fibroblast growth factor-23 in patients with axial spondyloarthritis and its association with sclerostin, inflammation, and spinal damage

    DOI: 10.1007/s00296-019-04298-5

    PMID: 30968207

    Sample: Serum
  4. Medicina-Lithuania (2020) IF: 1.205
    Significance of acPWV for Survival of Hemodialysis Patients

    DOI: 10.3390/medicina56090435

    PMID: 32872092

    Sample: Serum
    Sclerostin and osteoprotegerin: new markers of chronic kidney disease mediated mineral and bone disease in children

    DOI: 10.1515/jpem-2020-0140

    Sample: serum
  6. Journal of Maternal-Fetal & Neonatal Medicine (2018) IF: 1.493
    Inhibitors of osteoblastogenesis in early human milk and maternal serum: evidence for protective properties of mother’s milk on bone

    DOI: 10.1080/14767058.2018.1514383

    Sample: Serum,Milk
  7. NEFROLOGIA (2020) IF: 1.544
    The correlation between sclerostin and bone mineral density in renal transplant recipients

    DOI: 10.1016/j.nefro.2020.04.009

    PMID: 32896441

    Sample: Serum
    Is serum sclerostin a marker of atherosclerosis in patients with chronic kidney disease–mineral and bone disorder?

    DOI: 10.1007/s11255-018-1935-5

    Sample: Serum
  9. Contemporary Clinical Dentistry (2021)
    Comparative Evaluation of Sclerostin Levels in Gingival Crevicular Fluid in the Treatment of Chronic Periodontitis Patients Using Diode Laser as an Adjunct to Scaling and Root Planing: A Clinico-biochemical Study

    DOI: 10.4103/ccd.ccd_19_20

    Sample: gingival crevicular fluid (GCF)
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