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Human sST2(Soluble ST2) ELISA Kit

Citations(12)Uniprot : Q01638

Cat.No.:E-EL-H6082
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.31-20 ng/mL
Sensitivity	0.19 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human sST2 in samples. No significant cross-reactivity or interference between Human sST2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human sST2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sST2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sST2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sST2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sST2. You can calculate the concentration of Human sST2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human sST2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human sST2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.82	2.46	9.50	0.95	2.17	9.26
Standard deviation	0.05	0.12	0.50	0.05	0.10	0.50
CV (%)	6.10	4.88	5.26	5.26	4.61	5.40

Recovery

The recovery of Human sST2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	100-110	104
EDTA plasma (n=8)	88-97	93
Cell culture media (n=8)	100-110	103

Linearity

Samples were spiked with high concentrations of Human sST2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q01638
Synonyms	IL1RL1, DER4, FIT-1, IL33R, ST2L, ST2V, T1, IL-1 R4
Research Area	Cancer, Cardiovascular, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

ALLERGOLOGY INTERNATIONAL (2023) IF: 7.478
Low serum soluble ST2 level in early childhood is associated with the risk for asthma at 7 years of age

DOI: 10.1016/j.alit.2022.07.002

Sample: serum
WORLD JOURNAL OF GASTROENTEROLOGY (2021) IF: 5.742
Serum soluble suppression of tumorigenicity 2 as a novel inflammatory marker predicts the severity of acute pancreatitis

DOI: 10.3748/wjg.v27.i38.6489

PMID: 34720537
Sample: serum
ALLERGOLOGY INTERNATIONAL (2018) IF: 4.045
Soluble ST2 suppresses IL-5 production by human basophilic KU812 cells, induced by epithelial cell-derived IL-33

DOI: 10.1016/j.alit.2018.05.009

Sample: Cell culture supernatant
EUROPEAN JOURNAL OF NEUROLOGY (2020) IF: 4.516
Serum soluble ST2 is a potential long‐term prognostic biomarker for transient ischaemic attack and ischaemic stroke

DOI: 10.1111/ene.14419

Sample: Serum
Clinical Respiratory Journal (2017) IF: 2.356
Changes in inflammatory mediators as a result of intermittent hypoxia in obstructive sleep apnea syndrome

DOI: 10.1111/crj.12718

Sample: Plasma
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY (2018) IF: 2.437
IL-33 and Its Receptor ST2 after Inhaled Allergen Challenge in Allergic Asthmatics

DOI: 10.1159/000488015

Sample: Plasma
HUMAN IMMUNOLOGY (2022) IF: 2.85
The function of IL-33/ST2 signaling axis in treg cells activating fibrosis in IgG4-related disease

DOI: 10.1016/j.humimm.2022.01.009

PMID: 35067387
Sample: Serum
Medicina-Lithuania (2022) IF: 2.948
Changes in Left Heart Geometry, Function, and Blood Serum Biomarkers in Patients with Obstructive Sleep Apnea after Treatment with Continuous Positive Airway Pressure

DOI: 10.3390/medicina58111511

PMID: 36363468
Sample: serum
JOURNAL OF INVESTIGATIVE SURGERY (2016) IF: 1
The Role of Interleukin-33 as an Inflammatory Marker in Differential Diagnosis of Idiopathic Granulomatous Mastitis and Breast Cancer

DOI: 10.1080/08941939.2016.1240270

Sample: Plasma,Serum
Brazilian Journal of Cardiovascular Surgery (2021) IF: 1.312
Soluble ST2 as a Potential Biomarker in Pericardial Fluid of Coronary Artery Patients

DOI: 10.21470/1678-9741-2020-0317

Sample: PF,plasma
MEDICINE (2020) IF: 1.552
Soluble ST2 is associated with increased carotid intima-media thickness in patients with type 2 diabetes mellitus: A case-control study

DOI: 10.1097/MD.0000000000018940

Sample: Plasma
CARDIOLOGY (2022) IF: 1.869
Association of Soluble Suppression of Tumorigenicity 2 with New-Onset Atrial Fibrillation in Acute Myocardial Infarction

DOI: 10.1159/000524765

PMID: 35580569
Sample: blood

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