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Human sST2(Soluble ST2) ELISA Kit

  • Cat.No.:E-EL-H6082

  • Reactivity: Human

To Purchase E-EL-H6082

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T/24
Assay time 3.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human sST2 in samples. No significant cross-reactivity or interference between Human sST2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human sST2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sST2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sST2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sST2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sST2. You can calculate the concentration of Human sST2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 12 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 12 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human sST2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human sST2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.82 2.46 9.50 0.95 2.17 9.26
Standard deviation 0.05 0.12 0.50 0.05 0.10 0.50
CV (%) 6.10 4.88 5.26 5.26 4.61 5.40

Recovery

The recovery of Human sST2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 100-110 104
EDTA plasma (n=8) 88-97 93
Cell culture media (n=8) 100-110 103

Linearity

Samples were spiked with high concentrations of Human sST2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q01638
SynonymsIL1RL1, DER4, FIT-1, IL33R, ST2L, ST2V, T1, IL-1 R4
Research AreaCancer, Cardiovascular, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. JOURNAL OF INVESTIGATIVE SURGERY (2016) IF: 1
    The Role of Interleukin-33 as an Inflammatory Marker in Differential Diagnosis of Idiopathic Granulomatous Mastitis and Breast Cancer

    DOI: 10.1080/08941939.2016.1240270

    Sample: Plasma,Serum
  2. Brazilian Journal of Cardiovascular Surgery (2021) IF: 1.312
    Soluble ST2 as a Potential Biomarker in Pericardial Fluid of Coronary Artery Patients

    DOI: 10.21470/1678-9741-2020-0317

    Sample: Pf,Plasma
  3. MEDICINE (2020) IF: 1.552
    Soluble ST2 is associated with increased carotid intima-media thickness in patients with type 2 diabetes mellitus: A case-control study

    DOI: 10.1097/MD.0000000000018940

    Sample: Plasma
  4. CARDIOLOGY (2022) IF: 1.869
    Association of Soluble Suppression of Tumorigenicity 2 with New-Onset Atrial Fibrillation in Acute Myocardial Infarction

    DOI: 10.1159/000524765

    PMID: 35580569

    Sample: Blood
  5. Clinical Respiratory Journal (2017) IF: 2.356
    Changes in inflammatory mediators as a result of intermittent hypoxia in obstructive sleep apnea syndrome

    DOI: 10.1111/crj.12718

    Sample: Plasma
  6. INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY (2018) IF: 2.437
    IL-33 and Its Receptor ST2 after Inhaled Allergen Challenge in Allergic Asthmatics

    DOI: 10.1159/000488015

    Sample: Plasma
  7. HUMAN IMMUNOLOGY (2022) IF: 2.85
    The function of IL-33/ST2 signaling axis in treg cells activating fibrosis in IgG4-related disease

    DOI: 10.1016/j.humimm.2022.01.009

    PMID: 35067387

    Sample: Serum
  8. Medicina-Lithuania (2022) IF: 2.948
    Changes in Left Heart Geometry, Function, and Blood Serum Biomarkers in Patients with Obstructive Sleep Apnea after Treatment with Continuous Positive Airway Pressure

    DOI: 10.3390/medicina58111511

    PMID: 36363468

    Sample: Serum
  9. ALLERGOLOGY INTERNATIONAL (2018) IF: 4.045
    Soluble ST2 suppresses IL-5 production by human basophilic KU812 cells, induced by epithelial cell-derived IL-33

    DOI: 10.1016/j.alit.2018.05.009

    Sample: Cell Culture Supernatant
  10. EUROPEAN JOURNAL OF NEUROLOGY (2020) IF: 4.516
    Serum soluble ST2 is a potential long‐term prognostic biomarker for transient ischaemic attack and ischaemic stroke

    DOI: 10.1111/ene.14419

    Sample: Serum
  11. WORLD JOURNAL OF GASTROENTEROLOGY (2021) IF: 5.742
    Serum soluble suppression of tumorigenicity 2 as a novel inflammatory marker predicts the severity of acute pancreatitis

    DOI: 10.3748/wjg.v27.i38.6489

    PMID: 34720537

    Sample: Serum
  12. ALLERGOLOGY INTERNATIONAL (2023) IF: 7.478
    Low serum soluble ST2 level in early childhood is associated with the risk for asthma at 7 years of age

    DOI: 10.1016/j.alit.2022.07.002

    Sample: Serum
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