Click here to view cell apoptosis assay products

Human TIMP-1(Tissue Inhibitors of Metalloproteinase 1) ELISA Kit

  • Cat.No.:E-EL-H0184

  • Reactivity: Human

To Purchase E-EL-H0184

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human TIMP-1 in samples. No significant cross-reactivity or interference between Human TIMP-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human TIMP-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TIMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TIMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TIMP-1. You can calculate the concentration of Human TIMP-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TIMP-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TIMP-1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 1.40 3.60 0.50 1.40 3.50
Standard deviation 0.03 0.07 0.11 0.03 0.06 0.11
CV (%) 6.00 5.00 3.06 6.00 4.29 3.14


The recovery of Human TIMP-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-107 99
EDTA plasma (n=8) 89-102 95
Cell culture media (n=8) 93-107 99


Samples were spiked with high concentrations of Human TIMP-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P01033
Research AreaCancer, Cell Biology, Cardiovascular, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

    Tumor-Contacted Neutrophils Promote Metastasis by a CD90-TIMP-1 Juxtacrine–Paracrine Loop

    DOI: 10.1158/1078-0432.CCR-18-2544

    Sample: tumor-infiltrating neutrophils (TINs),neutrophils
    Stiff substrates inhibit collagen accumulation via integrin α2β1, FAK and Src kinases in human atrial fibroblast and myofibroblast cultures derived from patients with aortal stenosis

    DOI: 10.1016/j.biopha.2023.114289

    Sample: cardiac fibroblast
  3. Antioxidants (2022) IF: 7.675
    Anti-Aging Effect and Mechanism of Proanthocyanidins Extracted from Sea buckthorn on Hydrogen Peroxide-Induced Aging Human Skin Fibroblasts

    DOI: 10.3390/antiox11101900

    PMID: 36290623

    Sample: HSFs
  4. Cells (2021) IF: 6.6
    The Stiffness of Cardiac Fibroblast Substrates Exerts a Regulatory Influence on Collagen Metabolism via α2β1 Integrin, FAK and Src Kinases

    DOI: 10.3390/cells10123506

    Sample: cardiac fibroblast
  5. Frontiers in Immunology (2020) IF: 5.085
    Infection of Mycobacterium tuberculosis Promotes Both M1/M2 Polarization and MMP Production in Cigarette Smoke-Exposed Macrophages

    DOI: 10.3389/fimmu.2020.01902

    PMID: 32973788

    Sample: Serum
    A novel glyceroglycolipid from brown algae Ishige okamurae improve photoaging and counteract inflammation in UVB-induced HaCaT cells

    DOI: 10.1016/j.cbi.2021.109737

    PMID: 34740599

    Sample: HaCaT cells
    Preparation and evaluation of photoprotective kenaf seed oil nanocarriers-based cream of tocotrienol-rich fraction

    DOI: 10.1016/j.indcrop.2022.115164

    Sample: Normal human dermal fibroblasts (NHDF)
    Human arsenic exposure and lung function impairment in coal-burning areas in Guizhou, China

    DOI: 10.1016/j.ecoenv.2020.110174

    Sample: serum
  9. Journal of Functional Foods (2020) IF: 3.701
    Trehalose against UVB-induced skin photoaging by suppressing MMP expression and enhancing procollagen I synthesis in HaCaT cells

    DOI: 10.1016/j.jff.2020.104198

    Sample: HaCaT cell
  10. Biomed Research International (2020) IF: 2.276
    Effects of 12-Week Exercise Program on Enzyme Activity of Serum Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in Female Patients with Postmenopausal Osteoporosis: A Randomized Control Study

    DOI: 10.1155/2020/9758289

    Sample: serum
  11. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Mediators of extracellular matrix degradation and inflammation: A new team of possible biomarkers for oral squamous cell carcinoma stage

    DOI: 10.3892/etm.2021.10309

    PMID: 34194555

    Sample: Oral cancer tissue
    Efficacy of Proanthocyanidins in Nonsurgical Periodontal Therapy

    DOI: 10.1016/j.identj.2022.08.006

    PMID: 36167610

    Sample: saliva samples
  13. BMC Oral Health (2021) IF: 2.757
    The effect of conventional versus electronic cigarette use on treatment outcomes of peri-implant disease

    DOI: 10.1186/s12903-021-01784-w

    Sample: Saliva
  14. ANDROLOGIA (2022) IF: 2.775
    Relationship of interleukin-23 with matrix metalloproteinase-9, pentraxin-3, sleep quality and prostate size in benign prostatic hyperplasia

    DOI: 10.1111/and.14387

    PMID: 35092070

    Sample: serum
  15. MEDICINE (2020) IF: 1.552
    Dominative role of MMP-14 over MMP-15 in human urinary bladder carcinoma on the basis of its enhanced specific activity

    DOI: 10.1097/MD.0000000000019224

    PMID: 32049862

    Sample: Tissue homogenate
Show AllShorten


  • Show all
  • Reviews
  • Q&A

Verified Customer

h********nSubmitted [ Jun 14 2019 ]

  • Species:Human
  • Sample:serum and plasma
  • Description:The arrival of the Human TIMP-1 ELISA Kit is faster than I’ve expected . My dilution ratio of the serum and plasma is not accurate at first . luckily , I found the correct dilution ratio trying twice . I should have done more dilution ratio to save time at first . but the Kit of Human TIMP-1 is so sample to use . the datasheet of human TIMP-1 kit is useful in detail , and I am very satisfied with the results . I’ll consider the company in future research.
Read More
Read Less

S***zSubmitted [ Jun 16 2022 ]

Asked: Hello, I will use E-EL-H0184 kit with the cell culture media. Is there any dilution range that you recommend for cell culture media samples??



adminSubmitted [ Jun 16 2022 ]


... Show All Show Less

People Also Bought

Apply for Free Shipping 24T ELISA Kit
*Product Name:
*Catalog Number:
*When will you use it?