Current Position:Home>>Kits>>ELISA Kits>> Human TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
Cat.No.:E-EL-H0109
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Add to cart Bulk requestAssay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 7.81-500 pg/mL |
Sensitivity | 4.69 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human TNF-α in samples. No significant cross-reactivity or interference between Human TNF-α and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human TNF-α concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TNF-α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TNF-α were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 24.10 | 79.50 | 183.00 | 22.70 | 86.50 | 177.40 |
Standard deviation | 1.50 | 3.80 | 9.70 | 1.20 | 4.00 | 7.60 |
CV (%) | 6.22 | 4.78 | 5.30 | 5.29 | 4.62 | 4.28 |
The recovery of Human TNF-α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 86-100 | 92 |
EDTA plasma (n=8) | 94-106 | 99 |
Cell culture media (n=8) | 93-107 | 99 |
Samples were spiked with high concentrations of Human TNF-α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt: P01375 |
Synonyms | DIF, TNF-alpha, TNFA, TNFSF2 |
Research Area | Cancer,Immunology,Signaling transduction,Stem cells |
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1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
Cat.No. | Product Name | Specifications |
E-ELIR-001 | ELISA Plate Coating Buffer(1×) | 100mL, 1000mL |
E-ELIR-002 | ELISA Plate Coating Buffer(5×) | 100mL, 1000mL |
E-ELIR-003 | ELISA Plate Blocking Buffer | 100mL, 1000mL |
E-ELIR-004 | Wash Buffer for Sandwich-ELISA(25×) | 100mL, 1000mL |
E-ELIR-005 | Wash Buffer for Competitive-ELISA(25×) | 100mL, 1000mL |
E-ELIR-006 | Stop Solution | 100mL, 1000mL |
E-ELIR-007 | HRP-conjugate Stabilizer | 10mL, 100mL, 500mL |
E-ELIR-008 | HRP-conjugate Diluent | 100mL, 1000mL |
E-ELIR-009 | Biotinylated Antibody Stabilizer | 10mL, 100mL, 500mL |
E-ELIR-0010 | Biotinylated Antibody Diluent | 10mL, 100mL, 500mL |
E-ELIR-0011 | Sample Diluent | 10mL, 100mL, 500mL |
From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!
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