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Human TP53(Tumor Protein p53) ELISA Kit

  • Cat.No.:E-EL-H0910

  • Reactivity: Human

To Purchase E-EL-H0910

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Properties

Format 24T, 48T, 96T, 96T*5, 96T*10
Assay time 3.5h
Sample type serum, plasma and other biological fluids
Sample volume 100μL
Sample size Run up to 46 samples in duplicate and 30 samples in triplicate
Storage 2-8℃
Interpretation Detection range: 78.13-5000 pg/mL
Sensitivity: 46.88 pg/mL
Application Quantitative determination of Human S1RBD concentrations in human serum, plasma and other biological fluids
Reproducibility Both intra-CV and inter-CV are < 10%
Synonyms TP53, BCC7, LFS1, P53, TRP53, tumor protein p53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TP53. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TP53 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TP53, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TP53. You can calculate the concentration of Human TP53 in the samples by comparing the OD of the samples to the standard curve.

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Biochimie (2018) IF: 3.413
    Potential effects of metformin in DNA BER system based on oxidative status in type 2 diabetes

    DOI: 10.1016/j.biochi.2018.08.002

    PMID: 30098371

    Sample: Whole Blood
  2. BMC oral health (2020) IF: 1.911
    Suitability of MDA, 8-OHdG and wild-type p53 as genotoxic biomarkers in metal (Co, Ni and Cr) exposed dental technicians: a cross-sectional study

    DOI: 10.1186/s12903-020-1049-1

    PMID: 32138726

    Sample: Saliva
  3. Human & Experimental Toxicology (2017) IF: 1.802
    Role Of Metformin On Base Excision Repair Pathway In p53 Wild-Type H2009 And HepG2 Cancer Cells.

    DOI: 10.1177/0960327117737145

    PMID: 29185360

    Sample: Cell lysate
  4. Dental Journal (2017)
    The Correlation Between The Use Of Personal Protective Equipment And Level Wild-Type P53 Of Dental Technicians In Surabaya.

    DOI: 10.20473/j.djmkg.v50.i1.p19-22

    Sample: Saliva
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