Human TP53(Tumor Protein p53) ELISA Kit

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    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
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      Catalog number:E-EL-H0910

      Synonyms:TP53, BCC7, LFS1, P53, TRP53, tumor protein p53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

      Size:
      • 96T
      • 48T
      • 24T
      • 96T*5
      • 96T*10
      Qty:
      - +
      Price: $609

      Reactivity: Human

      Detection Range: 78.13~5000 pg/mL

      Sensitivity: 46.88 pg/mL

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Intended use
      This ELISA kit applies to the in vitro quantitative determination of Human TP53 concentrations in serum, plasma, saliva and other biological fluids.

      Test principle
      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TP53. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TP53 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TP53, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TP53. You can calculate the concentration of Human TP53 in the samples by comparing the OD of the samples to the standard curve.


      Assay type

      Sandwich

      Format

      96T

      Assay time

      3.5h

      Reactivity

      Human

      Detection Method

      Colormetric

      Detection Range

      78.13— 5000pg/mL

      Sensitivity

      46.88 pg/mL

      Sample Volume

      100μL

      Sample Type

      Serum, plasma, saliva and other biological fluids

      Specificity

      This kit recognizes in samples Human TP53. No Significant cross-reactivity or interference between Human TP53 and analogues was observed.



      Kit components & Storage


      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received


      Item

      Specifications

      Storage

      Micro ELISA Plate (Dismountable)

      8 wells ×12 strips

      -20, 6 months

      Reference Standard

      2 vials

      Concentrated Biotinylated Detection Ab (100×)

      1 vial, 120 uL

      Concentrated HRP Conjugate (100×)

      1 vial, 120 μL

      -20(shading light), 6 months

      Reference Standard & Sample Diluent

      1 vial, 20 mL

      4, 6 months

      Biotinylated Detection Ab Diluent

      1 vial, 14 mL

      HRP Conjugate Diluent

      1 vial, 14 mL

      Concentrated Wash Buffer (25×)

      1 vial, 30 mL

      Substrate Reagent

      1 vial, 10 mL

      4(shading light)

      Stop Solution

      1 vial, 10 mL

      4

      Plate Sealer

      5 pieces

      Product Description

      1 copy

      Certificate of Analysis

      1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.

              The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring 

              equipment instead of directly pouring into the vial(s).


      Other supplies required


      Microplate reader with 450 nm wavelength 

      filter High-precision transfer pipette, EP tubes and disposable pipette tips 

      Incubator capable of maintaining 37℃ 

      Deionized or distilled water 

      Absorbent paper 

      Loading slot for Wash Buffer




      Citations

      1. Potential effects of metformin in DNA BER system based on oxidative status in type 2 diabetes
        IF: 3.413
        Journal: Biochimie(2018)
        DOI: 10.1016/j.biochi.2018.08.002
        PMID: 30098371
        Products: E-EL-H0910 
        Sample type: Whole Blood
        Reactivity: Human
      2. Suitability of MDA, 8-OHdG and wild-type p53 as genotoxic biomarkers in metal (Co, Ni and Cr) exposed dental technicians: a cross-sectional study
        IF: 1.911
        Journal: BMC oral health(2020)
        DOI: 10.1186/s12903-020-1049-1
        PMID: 32138726
        Products: E-EL-H0910 
        Sample type: Saliva
        Reactivity: Human
      3. Role Of Metformin On Base Excision Repair Pathway In p53 Wild-Type H2009 And HepG2 Cancer Cells.
        IF: 1.802
        Journal: Human & Experimental Toxicology(2017)
        DOI: 10.1177/0960327117737145
        PMID: 29185360
        Products: E-EL-H0910 
        Sample type: Cell lysate
        Reactivity: Human
      4. The Correlation Between The Use Of Personal Protective Equipment And Level Wild-Type P53 Of Dental Technicians In Surabaya.
        Journal: Dental Journal(2017)
        DOI: 10.20473/j.djmkg.v50.i1.p19-22
        Products: E-EL-H0910 
        Sample type: Saliva
        Reactivity: Human
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