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Human VCAM-1/CD106(Vascular Cell Adhesion Molecule 1) ELISA Kit

Citations(11)Uniprot : P19320

Cat.No.:E-EL-H5587
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	1.56-100 ng/mL
Sensitivity	0.94 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human VCAM-1/CD106 in samples. No significant cross-reactivity or interference between Human VCAM-1/CD106 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human VCAM-1/CD106 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VCAM-1/CD106. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VCAM-1/CD106, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VCAM-1/CD106. You can calculate the concentration of Human VCAM-1/CD106 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human VCAM-1/CD106 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human VCAM-1/CD106 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	4.82	11.41	47.65	5.08	11.96	47.72
Standard deviation	0.27	0.50	2.48	0.28	0.50	2.07
CV (%)	5.60	4.38	5.20	5.51	4.18	4.34

Recovery

The recovery of Human VCAM-1/CD106 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	83-98	90
EDTA plasma (n=8)	94-105	100
Cell culture media (n=8)	94-110	101

Linearity

Samples were spiked with high concentrations of Human VCAM-1/CD106 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P19320
Research Area	Cancer, Cardiovascular, Neuroscience, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Antioxidants (2021) IF: 6.313
Urolithiasis Develops Endothelial Dysfunction as a Clinical Feature

DOI: 10.3390/antiox10050722

PMID: 34064366
Sample: Serum
Nutrients (2021) IF: 5.719
Rapid and Effective Vitamin D Supplementation May Present Better Clinical Outcomes in COVID-19 (SARS-CoV-2) Patients by Altering Serum INOS1, IL1B, IFNg, Cathelicidin-LL37, and ICAM1

DOI: 10.3390/nu13114047

PMID: 34836309
Sample: serum
JOURNAL OF PROTEOME RESEARCH (2021) IF: 4.466
Proteomic Profiling of Leukocytes Reveals Dysregulation of Adhesion and Integrin Proteins in Chronic Kidney Disease-Related Atherosclerosis

DOI: 10.1021/acs.jproteome.0c00883

PMID: 33939431
Sample: Cell culture supernatant,Plasma
Nutrients (2020) IF: 4.546
The Effects of Tocotrienol-Rich Vitamin E (Tocovid) on Diabetic Neuropathy: A Phase II Randomized Controlled Trial

DOI: 10.3390/nu12051522

PMID: 32456230
Sample: Serum
Oxidative Medicine and Cellular Longevity (2019) IF: 4.868
Antitumoral and Immunomodulatory Effect of Mahonia aquifolium Extracts

DOI: 10.1155/2019/6439021

Sample: A2780 cell,A375 cell,BJ cell,DLD-1 cell
DYES AND PIGMENTS (2022) IF: 4.889
New methylene blue analogues with N-piperidinyl-carbinol units: Synthesis, optical properties and in vitro internalization in human ovarian cancer cells

DOI: 10.1016/j.dyepig.2022.110460

Sample: A2780 cell,OVCAR-3 cell,BJ cell
Therapeutic Advances in Endocrinology and Metabolism (2019) IF: 3.543
Tocotrienol-rich vitamin E improves diabetic nephropathy and persists 6–9 months after washout: a phase IIa randomized controlled trial:

DOI: 10.1177/2042018819895462

Sample: Serum
BRITISH JOURNAL OF NUTRITION (2022) IF: 3.718
Brazil and cashew nuts intake improve body composition and endothelial health in women at cardiometabolic risk (Brazilian Nuts Study): a randomized controlled trial

DOI: 10.1017/S000711452100475X

PMID: 35193718
Sample: serum
Biomarkers in Medicine (2016) IF: 2.179
The association of plasma miR-155 and VCAM-1 levels with coronary collateral circulation

DOI: 10.2217/bmm-2016-0282

Sample: Plasma
Experimental and Therapeutic Medicine (2023) IF: 2.751
NEDD4 attenuates oxidized low‑density lipoprotein‑induced inflammation and dysfunction in vascular endothelial cells via regulating APEX1 expression

DOI: 10.3892/etm.2023.11787

Sample: U937 monocytes,human umbilical vein endothelial ce
LIFE SCIENCES (2017) IF: 2.936
The relationship of plasma decoy receptor 3 and coronary collateral circulation in patients with coronary artery disease

DOI: 10.1016/j.lfs.2017.09.025

Sample: Plasma

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