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Human VCAM-1/CD106(Vascular Cell Adhesion Molecule 1) ELISA Kit

  • Cat.No.:E-EL-H5587

  • Reactivity: Human

To Purchase E-EL-H5587

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 1.56-100 ng/mL
Sensitivity 0.94 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human VCAM-1/CD106 in samples. No significant cross-reactivity or interference between Human VCAM-1/CD106 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human VCAM-1/CD106 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VCAM-1/CD106. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VCAM-1/CD106, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VCAM-1/CD106. You can calculate the concentration of Human VCAM-1/CD106 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human VCAM-1/CD106 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human VCAM-1/CD106 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 4.82 11.41 47.65 5.08 11.96 47.72
Standard deviation 0.27 0.50 2.48 0.28 0.50 2.07
CV (%) 5.60 4.38 5.20 5.51 4.18 4.34


The recovery of Human VCAM-1/CD106 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 83-98 90
EDTA plasma (n=8) 94-105 100
Cell culture media (n=8) 94-110 101


Samples were spiked with high concentrations of Human VCAM-1/CD106 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P19320
Research AreaCancer, Cardiovascular, Neuroscience, Signal transduction, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


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  1. Antioxidants (2021) IF: 6.313
    Urolithiasis Develops Endothelial Dysfunction as a Clinical Feature

    DOI: 10.3390/antiox10050722

    PMID: 34064366

    Sample: Serum
  2. Nutrients (2021) IF: 5.719
    Rapid and Effective Vitamin D Supplementation May Present Better Clinical Outcomes in COVID-19 (SARS-CoV-2) Patients by Altering Serum INOS1, IL1B, IFNg, Cathelicidin-LL37, and ICAM1

    DOI: 10.3390/nu13114047

    PMID: 34836309

    Sample: serum
    Proteomic Profiling of Leukocytes Reveals Dysregulation of Adhesion and Integrin Proteins in Chronic Kidney Disease-Related Atherosclerosis

    DOI: 10.1021/acs.jproteome.0c00883

    PMID: 33939431

    Sample: Cell culture supernatant,Plasma
  4. Nutrients (2020) IF: 4.546
    The Effects of Tocotrienol-Rich Vitamin E (Tocovid) on Diabetic Neuropathy: A Phase II Randomized Controlled Trial

    DOI: 10.3390/nu12051522

    PMID: 32456230

    Sample: Serum
  5. Oxidative Medicine and Cellular Longevity (2019) IF: 4.868
    Antitumoral and Immunomodulatory Effect of Mahonia aquifolium Extracts

    DOI: 10.1155/2019/6439021

    Sample: A2780 cell,A375 cell,BJ cell,DLD-1 cell
  6. DYES AND PIGMENTS (2022) IF: 4.889
    New methylene blue analogues with N-piperidinyl-carbinol units: Synthesis, optical properties and in vitro internalization in human ovarian cancer cells

    DOI: 10.1016/j.dyepig.2022.110460

    Sample: A2780 cell,OVCAR-3 cell,BJ cell
  7. Therapeutic Advances in Endocrinology and Metabolism (2019) IF: 3.543
    Tocotrienol-rich vitamin E improves diabetic nephropathy and persists 6–9 months after washout: a phase IIa randomized controlled trial:

    DOI: 10.1177/2042018819895462

    Sample: Serum
    Brazil and cashew nuts intake improve body composition and endothelial health in women at cardiometabolic risk (Brazilian Nuts Study): a randomized controlled trial

    DOI: 10.1017/S000711452100475X

    PMID: 35193718

    Sample: serum
  9. Biomarkers in Medicine (2016) IF: 2.179
    The association of plasma miR-155 and VCAM-1 levels with coronary collateral circulation

    DOI: 10.2217/bmm-2016-0282

    Sample: Plasma
  10. Experimental and Therapeutic Medicine (2023) IF: 2.751
    NEDD4 attenuates oxidized low‑density lipoprotein‑induced inflammation and dysfunction in vascular endothelial cells via regulating APEX1 expression

    DOI: 10.3892/etm.2023.11787

    Sample: U937 monocytes,human umbilical vein endothelial ce
  11. LIFE SCIENCES (2017) IF: 2.936
    The relationship of plasma decoy receptor 3 and coronary collateral circulation in patients with coronary artery disease

    DOI: 10.1016/j.lfs.2017.09.025

    Sample: Plasma
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