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Human VEGFR-2/KDR(Vascular Endothelial Growth Factor Receptor 2) ELISA Kit

  • Cat.No.:E-EL-H1603

  • Reactivity: Human

To Purchase E-EL-H1603

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 78.13-5000 pg/mL
Sensitivity 46.88 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human VEGFR-2/KDR in samples. No significant cross-reactivity or interference between Human VEGFR-2/KDR and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human VEGFR-2/KDR concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGFR-2/KDR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGFR-2/KDR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGFR-2/KDR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGFR-2/KDR. You can calculate the concentration of Human VEGFR-2/KDR in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human VEGFR-2/KDR were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human VEGFR-2/KDR were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 243.80 712.30 2220.00 232.10 733.70 2091.00
Standard deviation 14.90 30.60 119.90 12.30 40.40 102.50
CV (%) 6.11 4.30 5.40 5.30 5.51 4.90


The recovery of Human VEGFR-2/KDR spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-105 97
EDTA plasma (n=8) 93-109 101
Cell culture media (n=8) 88-103 94


Samples were spiked with high concentrations of Human VEGFR-2/KDR and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P35968
SynonymsVEGFR2, CD309, FLK1
Research AreaCancer, Cardiovascular, Metabolism, Microbiology, Signal transduction, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


  1. Arabian Journal of Chemistry (2021) IF: 5.165
    Inhibition effects of 7-phloro-eckol from Ecklonia cava on metastasis and angiogenesis induced by hypoxia through regulation of AKT/mTOR and ERK signaling pathways

    DOI: 10.1016/j.arabjc.2021.103187

    Sample: Cell Lysate
    Serum Levels of Angiogenic Factors Distinguish Between Women with Preeclampsia and Normotensive Pregnant Women But Not Severity of Preeclampsia in an Obstetric Center in Turkey

    DOI: 10.12659/MSM.915092

    PMID: 31522187

    Sample: Serum
    Synthesis, crystal structure, cytotoxicity and evaluation of the 6-oxo-6H-pyrrolo[3,2,1-ij]quinoline-5-carbaldehydes for inhibitory effect against protein kinases (VEGFR-2 and EGFR) and cyclooxygenase-2 (COX-2) activities

    DOI: 10.1016/j.molstruc.2020.128907

    Sample: Mda-Mb-231 Cell
  4. BIOSCIENCE REPORTS (2020) IF: 2.942
    Tim-3 promotes tube formation and decreases tight junction formation in vascular endothelial cells

    DOI: 10.1042/BSR20202130

    PMID: 33015716

    Sample: Hmvecs,Huvecs
    Anti-angiogenic effect of Shikonin in rheumatoid arthritis by downregulating PI3K/AKT and MAPKs signaling pathways

    DOI: 10.1016/j.jep.2020.113039

    PMID: 32497675

    Sample: Serum,Cell Culture Supernatant
  6. Chinese Journal of Cancer Research (2020) IF: 4.135
    Tim-3 promotes cell aggressiveness and paclitaxel resistance through NF-κB/STAT3 signalling pathway in breast cancer cells

    DOI: 10.21147/j.issn.1000-9604.2020.05.02

    PMID: 33223752

    Sample: Cell Culture Supernatant
    Mechanism of two alkaloids isolated from coral endophytic fungus for suppressing angiogenesis in atherosclerotic plaque in HUVEC

    DOI: 10.1016/j.intimp.2022.108931

    PMID: 35704971

    Sample: Huvec
  8. BIOORGANIC CHEMISTRY (2021) IF: 5.275
    Pharmacophore based drug design and synthesis of oxindole bearing hybrid as anticancer agents

    DOI: 10.1016/j.bioorg.2021.105358

    PMID: 34544029

    Sample: A431 Cell
    Mechanism Analysis of Antiangiogenic d-Isofloridoside from Marine Edible Red algae Laurencia undulata in HUVEC and HT1080 cell

    DOI: 10.1021/acs.jafc.1c05007

    PMID: 34767715

    Sample: Ht1080 Cells,Huvec Cells
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  • Q&A

M***************lSubmitted [ Jul 14 2022 ]

Asked: what is the normal range of Human VEGF R2/KDR in pg/mL ?



adminSubmitted [ Jul 14 2022 ]

Answered: For the kit E-EL-H1603, the reference value of our internal human serum/plasma sample is 16-27ng/mL, and it is recommended to dilute 50-100 times for detection.

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